Abstract
A novel strain with antifungal activity against Sclerotinia sclerotiorum was isolated from soil, and identified as Streptomyces sp. ZJUT-IFE-354 using morphological and 16S rDNA sequence analysis. The bioactive metabolite produced by strain ZJUT-IFE-354 was identified and characterized as valinomycin by spectroscopic and chemical methods. The yield of valinomycin was 191.26 mg/L from the culture of Streptomyces sp. ZJUT-IFE-354, which was the highest yield to our knowledge. The in vitro antifungal activity of valinomycin against S. sclerotiorum was investigated as 0.056 ± 0.012 (EC50) and 0.121 ± 0.023 μg/mL (EC95), respectively, which was approximately 10.696- and 30.960-fold more active than that of carbendazim. The results from scanning electron microscopy, cell membrane permeability, and D-sorbitol and ergosterol assay indicated that valinomycin exerted the antifungal activity probably by increasing permeability of fungal cell membrane, leading to mycelial electrolyte leakage, and eventually resulting in the death of S. sclerotiorum. Thus, valinomycin may be a promising antifungal agent to control S. sclerotiorum.
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Acknowledgements
The work was financially supported by the National Natural Science Foundation of China (Grant No. 21572206) and National Key Research and Development Program of Zhejiang Province (Grant No. 2019C02088).
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DZ, ZM, and XLC designed and conducted the experiments. DZ, HCC, WLM, JWZ, QW, and CM performed the experiments and analyzed the data. DZ drafted the manuscript. ZM, HCC, and YLL checked the language. The final manuscript was read and approved by all authors.
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Zhang, D., Ma, Z., Chen, H. et al. Efficient production of valinomycin by the soil bacterium, Streptomyces sp. ZJUT-IFE-354. 3 Biotech 12, 2 (2022). https://doi.org/10.1007/s13205-021-03055-5
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DOI: https://doi.org/10.1007/s13205-021-03055-5