Abstract
In this study, Ocimum basilicum (a proven broad spectrum medicinal plant for broad-spectrum pharmacological activities) leaf extract was used as conjugates for the fabrication of silver nanoparticles (AgNP). Color change of the reaction mixture and UV–Visible spectrophotometry indicated the fabrication of silver nanoparticles, further X-ray diffraction (XRD) crystallography, scanning electron microscopy (SEM), transmission electron microscopic images (TEM), and Selected area electron diffraction (SAED) confirms the purity, monodispersity, and morphology including size (22.4 nm) and conjugated functional group of Ocimum basilicum. The conjugation of functional OH, N–O, and C=O groups was confirmed by Fourier-transform infrared spectroscopy (FT-IR). The engineered AgNP have shown significantly efficient antibacterial and antibiofilm activities (92.7% biofilm inhibition) on diverse clinical strains and thus showed its potential for use in clinical applications.
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Acknowledgements
Lakshmanan Muthulakshmi acknowledges the Department of Science and Technology–Science and Engineering Research Board (PDF/2017/001574), Government of India, for the award of a National Postdoctoral Fellowship. The authors acknowledge the financial assistance of DST-PURSE –MKU for the SEM facility. SR and ND acknowledge the University of Johannesburg, South Africa to provide the research facilities.
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Muthulakshmi, L., Vijayakumar, T., Selvam, P. et al. Strong and nonspecific synergistic antibacterial/antibiofilm impact of nano-silver biosynthesized and decorated with active ingredients of Oscimum basilicum L.. 3 Biotech 11, 153 (2021). https://doi.org/10.1007/s13205-021-02687-x
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DOI: https://doi.org/10.1007/s13205-021-02687-x