Abstract
The strain Bifidobacterium animalis 01, isolated from centenarians, showed promising antioxidant potential in our previous studies. In this study, the genome information on strain 01 and the important antioxidant components are presented. The complete genome comprises a single circular chromosome (1,931,632 bp; 60.49% G + C content) with 1569 coding DNA sequences, 52 tRNA, and 9 rRNA operons. Based on phylogenomic analyses, strain 01 was designated as B. animalis subsp. lactis 01. The genomic analysis reveals that at least eight protein-coding genes are antioxidant-related genes. The conditions for simulating the oxidative stress have been determined. The results of quantitative reverse transcription PCR further demonstrated that the genes encoding the thioredoxin system (ahpC, ahpF, bcp, trxB, trxA, nrdH, and msrAB) and non-enzyme factors of the divalent cation transporter gene (mntH) were upregulated under the H2O2 challenge, indicating that the eight genes were effective components of the antioxidant system. The results of this study could benefit for understanding the antioxidant mechanism of B. animalis 01 and future utilization of it as a potential antioxidant agent.
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Abbreviations
- ROS:
-
Reactive oxygen species
- PCR:
-
Polymerase chain reaction
- RT-qPCR:
-
Real-time quantitative PCR
- COG:
-
Clusters of orthologous groups
- CFU:
-
Colony-forming units
- CDS:
-
Coding DNA sequences
- RAST:
-
Rapid annotation using subsystem technology
- GIT:
-
Gastrointestinal tract
- CICC:
-
China Center of Industrial Culture Collection
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This project was funded by the National Natural Science Foundation of China (31671831 and 31471707).
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JLZ and PLL designed the experiments. JLZ and SBW performed the experiments. JLZ, ZZ, SBW, and YXQ analyzed the results and wrote the manuscript. All authors read and approved the final manuscript.
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Zhang, J., Wang, S., Zeng, Z. et al. The complete genome sequence of Bifidobacterium animalis subsp. lactis 01 and its integral components of antioxidant defense system. 3 Biotech 9, 352 (2019). https://doi.org/10.1007/s13205-019-1890-6
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DOI: https://doi.org/10.1007/s13205-019-1890-6