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Transcriptomic profiles of human HaCaT cells in response to angelica polysaccharide

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Abstract

The aim of this study was to explore the anti-psoriatic effect and potential mechanism of Angelica polysaccharide (APS) on an in vitro HaCaT cell model. MTS assay was performed to determine whether APS has the ability to inhibit the proliferation of HaCaT cells. RNA-sequencing (RNA-seq) was performed to investigate the underlying mechanism of APS. Quantitative real-time PCR (qRT-PCR) was used to verify the accuracy of RNA-seq data. Our MTS assay results demonstrated that APS time- and concentration-dependently inhibits the proliferation of HaCaT cells. The anti-proliferation property of APS suggests that APS may have anti-psoriatic effect. In the RNA-seq part, comparison between the CK group (i.e., Control group) and ASP groups revealed dramatic differences [468 differentially expressed genes (DEGs) for CK group vs. ASP50 group; 563 DEGs for CK group vs. ASP100 group; 532 DEGs for CK group vs. ASP200 group]. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrich analysis performed on all DEGs failed to find any significant enriched GO terms or KEGG pathways to explain the anti-proliferative effect of APS. All DEGs were then classified into 20 expression profiles by trend analysis. Interestingly, cell proliferation-related GO terms were mostly dispersed in the profile 2 and 17. DEGs enriched in these terms were then analyzed. After literature retrieval, DEGs such as SERPINE1, SMAD6, CTGF, and TGF-β were suspected to closely relevant to the anti-proliferation effect of APS. qRT-PCR results showed similar expression trend with RNA-seq data for 8 out of 10 genes, indicating our sequence data are reliable.

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References

  • Chunshui Y, Yunzhu M, Jiangwei S, Fen X, Xiaojie D, Yan C, Xing C (2010) Experimental study of angelica polysaccharide induced apoptosis on HaCaT Cells. Chin J Dermato Venerol Integ Trad W Med 9(3):143–145

    Google Scholar 

  • Di Fusco D, Laudisi F, Dinallo V, Monteleone I, Di Grazia A, Marafini I, Troncone E, Colantoni A, Ortenzi A, Stolfi C (2017) Smad7 positively regulates keratinocyte proliferation in psoriasis. Brit J Dermatol 177(6):1633–1643

    Article  Google Scholar 

  • Endo H, Momota Y, Oikawa A, Shinkai H (2006) Psoriatic skin expresses the transcription factor Gli1: possible contribution of decreased neurofibromin expression. Brit J Dermatol 154(4):619–623

    Article  CAS  Google Scholar 

  • Farahnik B, Sharma D, Alban J, Sivamani R (2017) Oral (systemic) botanical agents for the treatment of psoriasis: a review. J Altern Complement Med 23(6):418–425

    Article  Google Scholar 

  • Hayakawa K, Ikeda K, Fujishiro M, Yoshida Y, Hirai T, Tsushima H, Miyashita T, Morimoto S, Suga Y, Takamori K (2018) Connective tissue growth factor neutralization aggravates the psoriasis skin lesion: the analysis of psoriasis model mice and patients. Ann Dermatol 30(1):47–53

    Article  CAS  Google Scholar 

  • Huang X, Lei Y, Guan H, Hao Y, Liu H, Sun G, Chen R, Song S (2017) Transcriptomic analysis of the regulation of stalk development in flowering Chinese cabbage (Brassica campestris) by RNA sequencing. Sci Rep UK 7(1):15517

    Article  Google Scholar 

  • Huang T-H, Lin C-F, Alalaiwe A, Yang S-C, Fang J-Y (2019) Apoptotic or antiproliferative activity of natural products against keratinocytes for the treatment of psoriasis. Int J Mol Sci 20(10):2558

    Article  Google Scholar 

  • Jiang M, Sun Z, Dang E, Li B, Fang H, Li J, Gao L, Zhang K, Wang G (2017) TGFβ/SMAD/microRNA-486-3p signaling axis mediates keratin 17 expression and keratinocyte hyperproliferation in psoriasis. J Invest Dermatol 137(10):2177–2186

    Article  CAS  Google Scholar 

  • Jing H, Sheng W (2006) Influence of angelica polysaccharide on apoptosis in keratinocytes of psoriasis-like lesions of guinea pigs. Med J Wuhan Univ 3(27):377–379

    Google Scholar 

  • Jing H, Sheng W, Duan D (2008) The influence of angelica polysaccharide on the expression levels of PCNA in psoriasis-like lesions of guinea pigs. Chin J Dermatovenereol 22(1):11–13

    CAS  Google Scholar 

  • Keseroglu HO, Gönül M (2014) Traditional topical herbal therapies in psoriasis. TANG 4(4):13–20

    Google Scholar 

  • Kushwaha A (2017) A review on alternative treatment of Psoriasis. J Pharm Res 11(7):864–877

    Google Scholar 

  • Li X-L, Wang Z-H, Zhao Y-X, Luo S-J, Zhang D-W, Xiao S-X, Peng Z-H (2012) Purification of a polysaccharide from Gynostemma pentaphyllum Makino and its therapeutic advantages for psoriasis. Carbohyd Polym 89(4):1232–1237

    Article  CAS  Google Scholar 

  • Li N, Zhao W, Xing J, Liu J, Zhang G, Zhang Y, Li Y, Liu W, Shi F, Bai Y (2017) Chinese herbal Pulian ointment in treating psoriasis vulgaris of blood-heat syndrome: a multi-center, double-blind, randomized, placebo-controlled trial. Bmc Complem Altern M 17(1):264

    Article  Google Scholar 

  • Liang W, Lin Z, Zhang L, Qin X, Zhang Y, Sun L (2017) Calcipotriol inhibits proliferation of human keratinocytes by downregulating STAT1 and STAT3 signaling. J Invest Med 65(2):376–381

    Article  Google Scholar 

  • Man X, Chen X, Li W, Landeck L, Dou T, Chen J, Zhou J, Cai S, Zheng M (2015) Analysis of epithelial–mesenchymal transition markers in psoriatic epidermal keratinocytes. Open Biol 5(8):1–8

    Article  Google Scholar 

  • Nielsen HJ, Christensen IJ, Svendsen M, Hansen U, Werther K, Brünner N, Petersen L, Kristensen J (2002) Elevated plasma levels of vascular endothelial growth factor and plasminogen activator inhibitor-1 decrease during improvement of psoriasis. Inflamm Res 51(11):563–567

    Article  CAS  Google Scholar 

  • Ota T, Takekoshi S, Takagi T, Kitatani K, Toriumi K, Kojima T, Kato M, Ikoma N, Mabuchi T, Ozawa A (2014) Notch signaling may be involved in the abnormal differentiation of epidermal keratinocytes in psoriasis. Acta Histochem Cytoc 47(4):175–183

    Article  CAS  Google Scholar 

  • Qu XA, Freudenberg JM, Sanseau P, Rajpal DK (2014) Integrative clinical transcriptomics analyses for new therapeutic intervention strategies: a psoriasis case study. Drug Discov Today 19(9):1364–1371

    Article  CAS  Google Scholar 

  • Saito A, Suzuki HI, Horie M, Ohshima M, Morishita Y, Abiko Y, Nagase T (2013) An integrated expression profiling reveals target genes of TGF-β and TNF-α possibly mediated by microRNAs in lung cancer cells. PLoS One 8(2):e56587

    Article  CAS  Google Scholar 

  • Sobolev V, Zolotorenko A, Soboleva A, Elkin A, Il’ina S, Serov D, Potekaev N, Tkachenko S, Minnibaev M, Piruzyan A (2011) Effects of expression of transcriptional factor AP-1 FOSL1 gene on psoriatic process. B Exp Biol Med 150(5):632–634

    Article  CAS  Google Scholar 

  • Tse W-P, Che C-T, Liu K, Lin Z-X (2006) Evaluation of the anti-proliferative properties of selected psoriasis-treating Chinese medicines on cultured HaCaT cells. J Ethnopharmacol 108(1):133–141

    Article  Google Scholar 

  • Wang J, Chen G, Shi T, Wang Y, Guan C (2019) Possible treatment for cutaneous lichen planus: an in vitro anti-inflammatory role of Angelica polysaccharide in human keratinocytes HaCaT. Int J Immunopathol Pharmacol 33:2058738418821837

    PubMed  PubMed Central  Google Scholar 

  • Weng S, Chen B, Wang Y, Liu C, Sun M, Chang C, Lin J, Yen H (2016) Traditional Chinese medicine use among patients with psoriasis in Taiwan: a nationwide population-based study. Evid Based Compl Alt 2:1–13

    Article  Google Scholar 

  • Wu P, Ma G, Zhu X, Gu T, Zhang J, Sun Y, Xu H, Huo R, Wang B, Shen B (2017) Cyr61/CCN1 is involved in the pathogenesis of psoriasis vulgaris via promoting IL-8 production by keratinocytes in a JNK/NF-κB pathway. Clin Immunol 174:53–62

    Article  CAS  Google Scholar 

  • Yu H, Mrowietz U, Seifert O (2009) Downregulation of SMAD2, 4 and 6 mRNA and TGFβ receptor I mRNA in lesional and non-lesional psoriatic skin. Acta Derm Venereol 89(4):351–356

    Article  CAS  Google Scholar 

  • Zhang CS, Yu JJ, Parker S, Zhang AL, May B, Lu C, Xue CC (2014) Oral Chinese herbal medicine combined with pharmacotherapy for psoriasis vulgaris: a systematic review. Int J Dermatol 53(11):1305–1318

    Article  CAS  Google Scholar 

  • Zhang C, Xu Q, Tan X, Meng L, Wei G, Liu Y, Zhang C (2017) Astilbin decreases proliferation and improves differentiation in hacat keratinocytes. Biomed Pharmacother 93:713–720

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from the Project of Sichuan Science and Technology Bureau (2016JY0214), the Scientific Research Project of Sichuan Health and Family Planning Commission (150243) and the Scientific Research Project of Suining Municipality (2015S23).

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Authors and Affiliations

  1. Department of Dermatology, Suining Central Hospital, No. 127, Western Desheng Road, Suining, 629000, China

    Chunshui Yu, Linli Liu, Qian Liao, Hanwen Wang, Fei Ouyang, Hui Fang, Yan Wan, Qinlian Lu & Tingting Liu

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  1. Chunshui Yu
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  2. Linli Liu
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  3. Qian Liao
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  4. Hanwen Wang
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Correspondence to Chunshui Yu.

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13205_2019_1828_MOESM1_ESM.xlsx

Supplement table S1. All DEGs after APS treatment as compared with the CK group. Genes with log2(FC)>1 and FDR<0.05 were up-regulated; Genes with log2(FC) <-1 and FDR<0.05 were down-regulated. (XLSX 182 kb)

Supplement table S2. Significantly enriched GO terms for all DEGs (XLSX 13 kb)

Supplement table S3.Significantly enriched GO terms for DEGs in all profiles (XLSX 21 kb)

Supplement table S4. Genes relevant to cell proliferation (XLSX 13 kb)

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Yu, C., Liu, L., Liao, Q. et al. Transcriptomic profiles of human HaCaT cells in response to angelica polysaccharide. 3 Biotech 9, 301 (2019). https://doi.org/10.1007/s13205-019-1828-z

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