Abstract
The genome of a wild-type solventogenic Clostridium sp. strain LJ4 that could directly convert undetoxified lignocellulosic hydrolysate to butanol and tolerate high concentration of furan and phenolic derivates occurring in the lignocellulosic hydrolysate is described. 16S rDNA gene sequencing and analysis indicated that it is closely related to Clostridium acetobutylicum. The genome size of strain LJ4 is 3.90 Mp, which has a G + C content of 30.72% and encodes 2711 proteins. It also has one 0.19 Mp plasmid with 181 predicted encoding proteins. Alcohol dehydrogenases (ADs) and a nicotinamide adenine dinucleotide phosphate (NADPH)-dependent flavin mononucleotide (FMN) reductase were identified, which may play key roles in inhibitors’ resistance in lignocellulosic hydrolysate.
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Acknowledgements
This work was supported by the Jiangsu Province Natural Science Foundation for Youths (BK20170993, BK20170997), the Project of State Key Laboratory of Materials-Oriented Chemical Engineering (KL16-08), the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (XTE1840), the Key Science and Technology Project of Jiangsu Province (BE2016389), and the National Natural Science Foundation of China (no. 21706125, no. 21727818, no. 21706124, no. 31700092).
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Nucleotide sequence accession numbers
This Whole Genome project has been deposited into GenBank. The chromosome is under the accession of CP030018 and the plasmid is under the accession of CP030019.
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Liu, J., Jiang, Y., Chen, T. et al. The draft genome sequence of Clostridium sp. strain LJ4 with high furan and phenolic derivates’ tolerances occurring from lignocellulosic hydrolysates. 3 Biotech 8, 406 (2018). https://doi.org/10.1007/s13205-018-1430-9
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DOI: https://doi.org/10.1007/s13205-018-1430-9