Skip to main content
SpringerLink
Log in

Endophytic fungi of Tinospora cordifolia with anti-gout properties

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

Targeting xanthine oxidase inhibition has been a prime strategy for exploration and development of non-purine based drugs for anti-hyperuricemic therapy. Recent studies from our laboratory have suggested that fungal endophytes from medicinal plants could play a pivotal role in exploration of novel therapeutic moieties. In the present study, 19 fungal endophytes were isolated from the medicinal plant Tinospora cordifolia and evaluated for their XO inhibitory activity. In the qualitative assay, Out of 19 fungal endophytes, 7 endophytes exhibited > 30% XO inhibition, of which isolates #1 TCSTITPLM, #53 TCSTITPLM, #105 TCSTITPLM and #83 TCSTITPLM were found to exhibit XO inhibition in the range of 38–45%. Further, ethyl acetate extract of #1TCSTITPLM exhibited 69% XO inhibitory activity followed by chloroform extract of #53 TCSTITPLM with 63% XOI activity in the in vitro uric acid estimation assay. In the quantitative NBT titer plate assay, both extracts exhibited 85% of XO inhibition. The present study is the first report of fungal endophytes isolated from T. cordifolia exhibiting XO inhibitory activity. Ethyl acetate and chloroform extract of #1 TCSTITPLM and #53 TCSTITPLM demonstrated potent XO inhibitory action. Further studies are warranted for purification and characterization of the XO inhibiting moieties.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Aggarwal A, Banerjee UC (2009) Screening of xanthine oxidase producing microorganisms using nitroblue tetrazolium based colorimetric assay method. Open Biotechnol J 3:46–49

    Article  CAS  Google Scholar 

  • Alam N, Yoon KN, Lee KR, Kim HY, Shin PG, Cheong JC, Yoo YB, Shim MJ, Lee MW, Lee TS (2011a) Assessment of antioxidant and phenolic compound concentrations as well as xanthine oxidase and tyrosinase inhibitory properties of different extracts of Pleurotus citrinopileatus fruiting bodies. Mycobiology 39(1):12–19

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Alam N, Yoon KN, Cha YJ, Kim JH, Lee RK, Lee TS (2011b) Appraisal of the antioxidant, phenolic compounds concentration, xanthine oxidase and tyrosinase inhibitory activities of Pleurotus salmoneostramineus. Afr J Agric Res 6(6):1555–1563

    Google Scholar 

  • Alam N, Yoon KN, Shin PG, Cheong JC, Yoo YB, Lee TS (2011c) Antioxidant, phenolic compounds concentration, xanthine oxidase and tyrosinase inhibitory activities of Pleurotus cornucopiae. Austr J Basic App Sci 5(3):229–239

    CAS  Google Scholar 

  • Alam N, Yoon KN, Lee TS (2011d) Evaluation of the antioxidant and antityrosinase activities of three extracts from Pleurotus nebrodensis fruiting bodies. Afr J Biotech 10(11):2978–2986

    CAS  Google Scholar 

  • Alsultanee IR, Ewadh MJ, Mohammed MF (2014) Novel natural anti gout medication extract from Momdica Charantia. J Nat Sci Res 4(17):16–23

    Google Scholar 

  • Aly AH, Debbab A, Kjer J, Proksch P (2010) Fungal endophytes from higher plants: a prolific source of phytochemicals and other bioactive natural products. Fungal Divers 41:1–16

    Article  Google Scholar 

  • Azmi SMN, Jamal P, Amid A (2012) Xanthine oxidase inhibitory activity from potential Malaysian medicinal plant as remedies for gout. Int Food Res J 19(1):159–165

    CAS  Google Scholar 

  • Berry CE, Hare JM (2004) Xanthine oxidoreductase and cardiovascular disease: molecular mechanisms and pathophysiological implications. J Physiol (Lond) 555:589–606

    Article  CAS  Google Scholar 

  • Billet L, Doaty S, Katz JD, Velasquez MT (2014). Review of hyperuricemia as new marker for metabolic syndrome. ISRN Rheumatol 2014:852–954

    Google Scholar 

  • Chang WS, Lee YJ, Lu FJ, Chiang HC (1993) Inhibitory effects of flavonoids on xanthine oxidase. Anticancer Res 13:2165–2170

    CAS  PubMed  Google Scholar 

  • Chowdhary K, Kaushik N (2015) Fungal endophyte diversity and bioactivity in the Indian medicinal plant Ocimum sanctum Linn. PLoS One 10(11):e0141444

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Desco MC, Asensi M, Marquez R, Martinez-Valls J, Vento M, Pallardo FV, Sastre J, Vina J (2002) Xanthine oxidase is involved in free radical production in type 1 diabetes: protection by allopurinol. Diabetes 51:1118–1124

    Article  CAS  PubMed  Google Scholar 

  • Dong LH, Fan SW, Ling QZ, Huang BB, Wei ZJ (2014) Indentification of huperzine A-producing endophytic fungi isolated from Huperzia serrata. World J Microbiol Biotechnol 30(3):1011–1017

    Article  CAS  PubMed  Google Scholar 

  • Gouda S, Das G, Sen SK, Shin HS, Patra JK (2016) Endophytes: a treasure house of bioactive compounds of medicinal importance. Front Microbiol 7:1538

    Article  PubMed  PubMed Central  Google Scholar 

  • Huang WY, Cai YZ, Hyde KD, Corke H, Sun M (2008) Biodiversity of endophytic fungi associated with 29 traditional Chinese medicinal plants. Fungal Divers 33:61–75

    Google Scholar 

  • Kapoor N, Saxena S (2014) Potential xanthine oxidase inhibitory activity of endophytic Lasiodiplodia pseudotheobromae. Appl Biochem Biotechnol 173:1360–1374

    Article  CAS  PubMed  Google Scholar 

  • Kapoor N, Saxena S (2016) Xanthine oxidase inhibitory and antioxidant potential of Indian Muscodor species. 3 Biotech 6:248

    Article  PubMed  PubMed Central  Google Scholar 

  • Kaul S, Gupta S, Ahmed M, Dhar K (2012) Endophytic fungi from medicinal plants: a treasure hunt for bioactive metabolites. Phytochem Rev 11(4):487–505

    Article  CAS  Google Scholar 

  • Lin SY, Wang CC, Lu YL, Wu WC, Hou WC (2008) Antioxidant, anti-semicarbazide-sensitive amine oxidase, and anti-hypertensive activities of geraniin isolated from Phyllanthus urinaria. Food Chem Toxicol 46:2485–2492

    Article  CAS  PubMed  Google Scholar 

  • Maheshwari R, Varahalarao V, Ramars A, Suryanarayana Murty U (2017) Endophytic fungi as novel resources of natural therapeutics. Braz Arch Biol Technol 60:e17160542

    Google Scholar 

  • Mitchell A, Strobel G, Hess W, Vargas P, Ezra D (2008) Muscodor crispans, a novel endophyte from Ananas ananassoides in the Bolivian Amazon. Fungal Divers 31:37–43

    Google Scholar 

  • Nair PK, Rodriguez S, Ramachandran R et al (2004) Immune stimulating properties of a novel polysaccharide from the medicinal plant Tinospora cordifolia. Int Immunopharmacol 4:1645–1659

    Article  CAS  PubMed  Google Scholar 

  • Nisa H, Kamili AN, Nawchoo IA, Shafi S, Shameem N, Bandh SA (2015) Fungal endophytes as prolific source of phytochemicals and other bioactive natural products: a review. Microb Pathog 82:50–59

    Article  CAS  PubMed  Google Scholar 

  • Raviraja NS, Maria GL, Sridhar KR (2006) Antimicrobial evaluation of endophytic fungi inhabiting medicinal plants of the Western ghats of India. Life Sci 6:515–520

    Article  CAS  Google Scholar 

  • Shu RG, Wang FW, Yang YM, Liu YX, Tan RX (2004) Antibacterial and xanthine oxidase inhibitory Cerebrosides from Fusarium sp. IFB-121, an endophytic fungus in Quercus variabilis. Lipids 39:667–673

    Article  CAS  PubMed  Google Scholar 

  • Sinha K, Mishra NP, Singh J, Khanuja SPS (2004) Tinospora cordifolia (Guduchi), a reservoir plant for therapeutic applications: a review. Indian J Trad Knowl 3(3):257–270

    Google Scholar 

  • Tripathi YB, Sharma M, Manickam M (1997) Rubiadin, a new antioxidant from Rubia cordifolia. Indian J Biochem Biophys 34:302–306

    CAS  PubMed  Google Scholar 

  • Warrier PK, Nambiar VPK, Ramankutty C, Nair RV (1996) Indian medicinal plants: a compendium of 500 species, vol 5, p 283

  • Whittaker RH (1977) Evolution of species diversity in land communities. Evol Biol 10:1–67

    Google Scholar 

  • Zanabaatar B, Song JH, Seo GS, Noh HJ, Yoo YB, Lee JS (2010) Screening of anti-gout xanthine oxidase inhibitor from mushrooms. Kor J Mycol 38(1):85–87

    Article  Google Scholar 

Download references

Acknowledgements

The authors are thankful to Department of Biotechnology (National Biodiversity Development Board) for financial assistance through project no. BT/PR/10083/NDB/52/95/2007. Further Prof. Sanjai Saxena is also thankful to DBT (National Biodiversity Board) for sponsored project specifically on XOI from endophytic fungi vide Project No. BT/PR9094/NDB/39/378/2013.

Author information

Author notes

  1. Neha Kapoor

    Present address: Department of Microbiology, Uttaranchal (PG) College of Biomedical Sciences and Hospital, Dehradun, Uttarakhand, 248002, India

Authors and Affiliations

  1. #BC-213, BC Link Block (First Floor) Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India

    Neha Kapoor & Sanjai Saxena

Authors
  1. Neha Kapoor
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sanjai Saxena
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sanjai Saxena.

Ethics declarations

Conflict of interest

The authors declare that there are no financial or commercial conflicts of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kapoor, N., Saxena, S. Endophytic fungi of Tinospora cordifolia with anti-gout properties. 3 Biotech 8, 264 (2018). https://doi.org/10.1007/s13205-018-1290-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-018-1290-3

Keywords

Search

Navigation