Skip to main content
SpringerLink
Log in

Mapping QTLs associated to germination stability following dry-heat treatment in rice seed

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

Using 164 recombinant inbred lines (RILs) derived from a cross between Milyang 23 (indica/japonica) and Gihobyeo (japonica) in rice, dry-heat tolerance was evaluated for the seeds of parents and RILs, whose dormancy was naturally broken in six months after harvesting. Mapping QTLs associated to dry-heat tolerance was carried out through interval mapping using Qgene 3.0. Seed germination after dry-heat treatments (90 °C for 24 h) showed a significant difference between the two parents, when evaluated for percentage germination and mean germination time. Milyang 23 was highly tolerant to the dry-heat treatment, while Gihobyeo was sensitive. Three QTLs (qDHT 1, qDHT 5, and qDHT 7) conferring the dry-heat tolerance were mapped to chromosomes 1, 5 and 7, respectively. qDHT 1 on chromosome 1 was tightly linked at 4 cM from ME1-1. The phenotypic variation explained by the three QTLs was 27.18% of the total variance in the 164 RIL populations, and the parental additive effects of three QTLs affected the Milyang 23 allele increased dry-heat tolerance. The detection of new QTLs associated with dry-heat tolerance will provide important information for disease and insect control, using dry-heat treatment in organic or low input sustainable agriculture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Cho YG, McCouch SR, Kuiper M, Kang MR, Pot J, Groenen JTM, Eun MY (1998) Integrated map of AFLP, SSLP and RFLP markers using a recombinant inbred population of rice (Oryza sativa L.). Theor Appl Genet 97:370–380

    Article  CAS  Google Scholar 

  • Coolbear P, Francis A, Grierson D (1984) The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds. Exp Bot 35:1609–1617

    Article  CAS  Google Scholar 

  • Dadlani M, Seshu DV (1990) Effect of wet and dry heat treatment on rice seed germination and seedling vigor. Int Rice Res Newsl 15(2):21–22

    Google Scholar 

  • Detry JF (1993) Seed dry-heat treatment against transmission of Pseudomonas fuscovagivae, causal agent of bacterial sheath brown rot of rice (BSR). Int Rice Res Notes 18(2):27–28

    Google Scholar 

  • Fourest E, Rehms LD, Sands DC, Bjarko M, Lund RE (1990) Eradication of Xanthomonas campestris pv. translucens from barley seed with dry heat treatments. Plant Dis 74:816–818

    Article  Google Scholar 

  • Guo L, Zhu L, Xu Y, Zeng D, Wu P, Qian Q (2004) QTL analysis of seed dormancy in rice (Oryza sativa L.). Euphytica 140:155–162

    Article  CAS  Google Scholar 

  • Hagiwara M, Imura M, Mitsuishi S (1990) Local soil reduction in the vicinity of the paddy rice seed coated with oxygen generating chemicals in relation to germination and emergence. Japan J Crop Sci 59:56–62

    Article  CAS  Google Scholar 

  • Juliano BO, Perez CM, Chang TT (1990) Varietal differences in longevity of tropical rough rice stored under ambient conditions. Seed Sci Technol 18:361–369

    Google Scholar 

  • Kim KM, Kwon YS, Lee JJ, Eun MY, Sohn JK (2004) QTL mapping and molecular marker analysis for the resistance of rice to ozone. Mol Cells 17(1):151–155

    Google Scholar 

  • Kwon YS, Kim KM, Eun MY, Sohn JK (2001) Quantitative trait loci mapping associated with plant regeneration ability from seed derived calli in rice (Oryza sativa L.). Mol Cells 11(1):64–67

    CAS  Google Scholar 

  • Lee SY, Lee JH, Kwon TO (2002) Varietal differences in seed germination and seedling vigor of Korean rice varieties following dry heat treatment. Seed Sci Technol 30:311–321

    Google Scholar 

  • Lee SY, Ahn JH, Kim HJ, Eun MY (2006a) Relationships among viviporous germination, dry-heat tolerance and seed longevity in Milyang 23/Gihobyeo RILs. Korean J Bioenviron Control 15:421–427

    Google Scholar 

  • Lee SY, Ahn JH, Cha YS, Yun DW, Lee MC, Eun MY (2006b) Mapping of quantitative trait loci associated with viviparous germination in rice. Korean J Crop Sci 51:565–570

    Google Scholar 

  • McCouch SR, Cho YG, Yano M, Paul E, Blinstrub M (1997) Report on QTL nomenclature. Rice Genet Newsl 14:11–13

    Google Scholar 

  • Morishima H, Oka HI (1981) Phylogenetic differentiation of cultivated rice, XXII. Numerical evaluation of the indica–japonica differentiation. Japan J Breed 31:402–413

    Article  Google Scholar 

  • Nakagawa A, Yamaguchi T (1989) Seed treatment for control of seed-borne Fusarium roseum on wheat. JARQ 23:94–99

    Google Scholar 

  • Nelson JC (1997) QGENE: software for marker-based genomic analysis and breeding. Mol Breeding 3:239–245

    Article  CAS  Google Scholar 

  • Roberts EH (1963) An investigation of inter-varietal differences in dormancy and viability of rice seed. Ann Bot 27:365–369

    Article  Google Scholar 

  • Sacheti U, Sacheti U (1996) Effect of extremely high temperature on the germination of seeds of some leguminous species and semi-arid areas of Oman. Indian J Ecol 23:29–33

    Google Scholar 

  • Scott SJ, Jones RA, Williams WA (1984) Review of data analysis methods for seed germination. Crop Sci 24:1160–1162

    Google Scholar 

  • Seshu DV, Dadlani M (1991) Mechanism of seed dormancy in rice. Seed Sci Res 1:187–194

    Article  CAS  Google Scholar 

  • Seshu DV, Sorells ME (1986) Genetic studies on seed dormancy in rice. Rice genetics, 1st edn. IRRI, Manila, pp 369–382

    Google Scholar 

  • Siddique SB (1988) Rice cultivar variability in tolerance for accelerated aging of seed. IRRI Res Pap Ser 131:2–7

    Google Scholar 

  • Tanksley SD (1993) Mapping polygenes. Annu Rev Genet 27:205–233

    Article  CAS  Google Scholar 

  • Zeigler RS, Ribiano M, Alvarez E (1987) Heat and chemical therapy to eradicated Pseudomonas fuscovaginae from rice seed. Int Rice Res Newsl 12(5):18–19

    Google Scholar 

  • Zhang XG (1990) Physiochemical treatments to break dormancy in rice. Int Rice Res Newsl 15(1):22

    Google Scholar 

Download references

Acknowledgements

This work was supported by Wonkwang University in 2017.

Author information

Authors and Affiliations

  1. Institute of Life Science and Natural Resources, Wonkwang University, Iksan, 54538, Korea

    Seung-Yeob Lee

  2. Division of Crop Science and Biotechnology, Dankook University, Cheonan, 31116, Korea

    Yong-Hwan Kim

  3. Biosafety Division, National Agricultural Science, 370 Nongsaengmyeong-ro, Jeonju, 54874, Korea

    Gang-Seob Lee

Authors
  1. Seung-Yeob Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yong-Hwan Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gang-Seob Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gang-Seob Lee.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lee, SY., Kim, YH. & Lee, GS. Mapping QTLs associated to germination stability following dry-heat treatment in rice seed. 3 Biotech 7, 220 (2017). https://doi.org/10.1007/s13205-017-0807-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-017-0807-5

Keywords

Search

Navigation