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Development of biopolymer-mediated nanocomposites using hot-melt extrusion to enhance the bio-accessibility and antioxidant capacity of kenaf seed flour

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Abstract

The current study was aimed to enhance the bio-accessibility and functionality of bioactive compounds from kenaf seed flour. Hence, eight different formulations (F1–F8) of kenaf seed nanocomposites (KNC) were prepared using lecithin, whey protein concentrated, and ascorbic acid by hot-melt extrusion (HME) at 80 °C, 100 °C, and 120 °C. Results revealed that F3 and F6 formulations at 100 °C were very promising; exhibited nano-size particle and dispersion, better water absorption-related parameters, and higher content of total polyphenol as well as total flavonoid. Besides, F3 and F6 also exhibited significant antioxidant potentials, evaluated by 1,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radical assays. The superior performances of F3 and F6 were evaluated by Fourier transform-infrared spectroscopy (FTIR) and scanning electron microscope (SEM). FTIR confirmed physical crosslinking generation between kenaf seed powder and biopolymers. Multiple water channels were identified through SEM analysis. Therefore, F3 and F6 formulations may be considered as noble formulations to improve bio-accessibility and antioxidant capacity of kenaf seed flour.

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References

  • Adnan M, Chy MNU, Rudra S, Tahamina A, Das R, Tanim MAH, Siddique TI, Hoque A, Tasnim SM, Paul A (2018) Evaluation of Bonamia semidigyna (Roxb.) for antioxidant, antibacterial, anthelmintic and cytotoxic properties with the involvement of polyphenols. Orient Pharm Exp Med 19:1–13

    Google Scholar 

  • Adnan M, Chy NU, Mostafa Kamal ATM, Azad MOK, Paul A, Uddin SB, Barlow JW, Faruque MO, Park CH, Cho DH (2019) Investigation of the biological activities and characterization of bioactive constituents of Ophiorrhiza rugosa var. prostrata (D. Don) & Mondal leaves through in vivo, in vitro, and in silico approaches. Molecules 24:1367

    Google Scholar 

  • Altan A, McCarthy KL, Maskan M (2008) Evaluation of snack foods from barley–tomato pomace blends by extrusion processing. J Food Eng 84:231–242

    Google Scholar 

  • Ashour EA, Majumdar S, Alsheteli A, Alshehri S, Alsulays B, Feng X, Gryczke A, Kolter K, Langley N, Repka MA (2016) Hot melt extrusion as an approach to improve solubility, permeability and oral absorption of a psychoactive natural product, piperine. J Pharm Pharmacol 68:989–998

    CAS  Google Scholar 

  • Asif M, Khodadadi E (2013) Medicinal uses and chemistry of flavonoid contents of some common edible tropical plants. J Paramed Sci 4:119–138

    Google Scholar 

  • Azad MOK, Piao JP, Park CH, Cho DH (2018) Far infrared irradiation enhances nutraceutical compounds and antioxidant properties in Angelica gigas Nakai powder. Antioxidants 7:189

    Google Scholar 

  • Azad MOK, Kim WW, Jin CW, Kang WS, Park CH, Cho DH (2019a) Development of a polymer-mediated soybean nanocomposite by hot melt extrusion to improve its functionality and antioxidant properties. Foods 8:41

    CAS  Google Scholar 

  • Azad MOK, Jeong DI, Adnan M, Salitxay T, Heo JW, Naznin MT, Lim JD, Cho DH, Park BJ, Park CH (2019b) Effect of different processing methods on the accumulation of the phenolic compounds and antioxidant profile of broomcorn millet (Panicum miliaceum L.). Flour Foods 8:230. https://doi.org/10.3390/foods8070230

    Article  CAS  Google Scholar 

  • Braca A, Fico G, Morelli I, De Simone F, Tomè F, De Tommasi N (2003) Antioxidant and free radical scavenging activity of flavonol glycosides from different Aconitum species. J Ethnopharmacol 86:63–67

    CAS  Google Scholar 

  • Chan KW, Khong NMH, Iqbal S, Mansor SM, Ismail M (2013) Defatted kenaf seed meal (DKSM): prospective edible flour from agricultural waste with high antioxidant activity. LWT Food Sci Technol 53:308–313

    CAS  Google Scholar 

  • Cinelli P, Schmid M, Bugnicourt E, Wildner J, Bazzichi A, Anguillesi I, Lazzeri A (2014) Whey protein layer applied on biodegradable packaging film to improve barrier properties while maintaining biodegradability. Polym Degrad Stab 108:151–157

    CAS  Google Scholar 

  • Crowley MM, Zhang F, Repka MA, Thumma S, Upadhye SB, Kumar Battu S, Martin C (2007) Pharmaceutical applications of hot-melt extrusion: part I. Drug Dev Ind Pharm 33(9):909–926

    CAS  Google Scholar 

  • Cui J, Zhang Z, Jiang H, Liu D, Zou L, Guo X, Lu Y, Parkin IP, Guo D (2019a) Ultrahigh recovery of fracture strength on mismatched fractured amorphous surfaces of silicon carbide. ACS Nano 13:7483–7492

    CAS  Google Scholar 

  • Cui J, Zhang Z, Liu D, Zhang D, Hu W, Zou L, Lu Y, Zhang C, Lu H, Tang C (2019b) Unprecedented piezoresistance coefficient in strained silicon carbide. Nano Lett 19:6569–6576

    CAS  Google Scholar 

  • de Castro RJS, Domingues MAF, Ohara A, Okuro PK, dos Santos JG, Brexo RP, Sato HH (2017) Whey protein as a key component in food systems: physicochemical properties, production technologies and applications. Food Struct 14:17–29

    Google Scholar 

  • Galanakis CM (2012) Recovery of high added-value components from food wastes: conventional, emerging technologies and commercialized applications. Trends Food Sci Technol 26:68–87

    CAS  Google Scholar 

  • Ghebremeskel AN, Vemavarapu C, Lodaya M (2007) Use of surfactants as plasticizers in preparing solid dispersions of poorly soluble API: selection of polymer–surfactant combinations using solubility parameters and testing the processability. Int J Pharm 328:119–129

    CAS  Google Scholar 

  • Ghimeray AK, Sharma P, Hu W, Cheng W, Park CH, Rho HS, Cho DH (2013) Far infrared assisted conversion of isoflavones and its effect on total phenolics and antioxidant activity in black soybean seed. J Med Plants Res 7:1129–1137

    CAS  Google Scholar 

  • Giwa Ibrahim S, Karim R, Saari N, Wan Abdullah WZ, Zawawi N, Ab Razak AF, Hamim NA, Umar RA (2019) Kenaf (Hibiscus cannabinus L.) Seed and its potential food applications: a review. J Food Sci 84(8):2015–2023

    CAS  Google Scholar 

  • Gui Y, Ryu G-H (2014) Effects of extrusion cooking on physicochemical properties of white and red ginseng (powder). J Ginseng Res 38:146–153

    CAS  Google Scholar 

  • Gülçin İ, Huyut Z, Elmastaş M, Aboul-Enein HY (2010) Radical scavenging and antioxidant activity of tannic acid. Arab J Chem 3:43–53

    Google Scholar 

  • Guo Z, Lu M, Li Y, Pang H, Lin L, Liu X, Wu C (2014) The utilization of drug–polymer interactions for improving the chemical stability of hot-melt extruded solid dispersions. J Pharm Pharmacol 66:285–296

    CAS  Google Scholar 

  • Gutiérrez TJ, Alvarez VA (2018) Bionanocomposite films developed from corn starch and natural and modified nano-clays with or without added blueberry extract. Food Hydrocoll 77:407–420

    Google Scholar 

  • Harito C, Bavykin DV, Yuliarto B, Dipojono HK, Walsh FC (2019) Polymer nanocomposites having a high filler content: synthesis, structures, properties, and applications. Nanoscale 11:4653–4682

    CAS  Google Scholar 

  • Hu Z, Tang X, Zhang M, Hu X, Yu C, Zhu Z, Shao Y (2018) Effects of different extrusion temperatures on extrusion behavior, phenolic acids, antioxidant activity, anthocyanins and phytosterols of black rice. RSC Adv 8:7123–7132

    CAS  Google Scholar 

  • Jeon S-M, Kim HK, Kim H-J, Do G-M, Jeong T-S, Park YB, Choi M-S (2007) Hypocholesterolemic and antioxidative effects of naringenin and its two metabolites in high-cholesterol fed rats. Transl Res 149:15–21

    CAS  Google Scholar 

  • Jurišić V, Julson JL, Krička T, Ćurić D, Voća N, Karunanithy C (2015) Effect of extrusion pretreatment on enzymatic hydrolysis of Miscanthus for the purpose of ethanol production. J Agric Sci 7:132

    Google Scholar 

  • Li Y, Pang H, Guo Z, Lin L, Dong Y, Li G, Lu M, Wu C (2014) Interactions between drugs and polymers influencing hot melt extrusion. J Pharm Pharmacol 66:148–166

    CAS  Google Scholar 

  • List GR (2015) Soybean lecithin: food, industrial uses, and other applications. In: Ahmad MU, Xu X (eds) Polar Lipids, Biology, Chemistry, and Technology, 1st edn. AOCS Press, Urbana, IL, pp 1–34

    Google Scholar 

  • Liu X, Lu M, Guo Z, Huang L, Feng X, Wu C (2012) Improving the chemical stability of amorphous solid dispersion with cocrystal technique by hot melt extrusion. Pharm Res 29:806–817

    CAS  Google Scholar 

  • Machado AR, de Assis LM, Machado MIR, de Souza-Soares LA (2014) Importance of lecithin for encapsulation processes. African J Food Sci 8:176–183

    Google Scholar 

  • Martin C (2008) Continuous mixing of solid dosage forms via hot-melt extrusion. Pharm Technol 32:76–86

    CAS  Google Scholar 

  • Martínez-Ballesta Mc, Gil-Izquierdo Á, García-Viguera C, Domínguez-Perles R (2018) Nanoparticles and controlled delivery for bioactive compounds: outlining challenges for new “Smart-foods” for health. Foods 7:72

    Google Scholar 

  • McClements DJ, Xiao H (2017) Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles. npj Sci Food 1:6

    Google Scholar 

  • Michotte D, Rogez H, Chirinos R, Mignolet E, Campos D, Larondelle Y (2011) Linseed oil stabilisation with pure natural phenolic compounds. Food Chem 129:1228–1231

    CAS  Google Scholar 

  • Miller DA, McConville JT, Yang W, Williams RO III, McGinity JW (2007) Hot-melt extrusion for enhanced delivery of drug particles. J Pharm Sci 96:361–376

    CAS  Google Scholar 

  • Mishra B, Arya N, Tiwari S (2010) Investigation of formulation variables affecting the properties of lamotrigine nanosuspension using fractional factorial design. DARU J Fac Pharmacy Tehran Univ Med Sci 18:1

    CAS  Google Scholar 

  • Moakes RJA, Sullo A, Norton IT (2015) Preparation and characterisation of whey protein fluid gels: the effects of shear and thermal history. Food Hydrocoll 45:227–235

    CAS  Google Scholar 

  • Nyam KL, Wong MM, Long K, Tan CP (2013) Oxidative stability of sunflower oils supplemented with kenaf seeds extract, roselle seeds extract and roselle extract, respectively under accelerated storage. Int Food Res J 20:695

    CAS  Google Scholar 

  • Patel A, Sahu D, Dashora A, Garg R, Agraval P, Patel Pratik, Patel Parth, Patel G (2013) A review of hot melt extrusion technique. Int J Innov Res Sci Eng Technol 2:2194–2198

    Google Scholar 

  • Piao J, Lee J-Y, Weon JB, Ma CJ, Ko H-J, Kim D-D, Kang W-S, Cho H-J (2015) Angelica gigas Nakai and Soluplus-based solid formulations prepared by hot-melting extrusion: oral absorption enhancing and memory ameliorating effects. PLoS One 10:e0124447

    Google Scholar 

  • Pielichowski K, Świerz-Motysia B (2005) Influence of polyesterurethane plasticizer on the kinetics of poly (vinyl chloride) decomposition process. J Therm Anal Calorim 83:207–212

    Google Scholar 

  • Repka MA, Bandari S, Kallakunta VR, Vo AQ, McFall H, Pimparade MB, Bhagurkar AM (2018) Melt extrusion with poorly soluble drugs–an integrated review. Int J Pharm 535:68–85

    CAS  Google Scholar 

  • Riedl KM, Hagerman AE (2001) Tannin–protein complexes as radical scavengers and radical sinks. J Agric Food Chem 49:4917–4923

    CAS  Google Scholar 

  • Rodriguez F, Cohen C, Ober CK, Archer L (2014) Principles of polymer systems. CRC Press, Boca Raton, McGraw-Hill, New York

    Google Scholar 

  • Ryu J, Ha B-K, Kim DS, Kim J-B, Kim SH, Kang S-Y (2013) Assessment of growth and seed oil composition of kenaf (Hibiscus cannabinus L.) germplasm. J Crop Sci Biotechnol 16:297–302

    Google Scholar 

  • Ryu J, Kwon S-J, Ahn J-W, Jo YD, Kim SH, Jeong SW, Lee MK, Kim J-B, Kang S-Y (2017) Phytochemicals and antioxidant activity in the kenaf plant (Hibiscus cannabinus L.). J Plant Biotechnol 44:191–202

    Google Scholar 

  • Saura-Calixto F, Serrano J, Goni I (2007) Intake and bioaccessibility of total polyphenols in a whole diet. Food Chem 101:492–501

    CAS  Google Scholar 

  • Sejidov FT, Mansoori Y, Goodarzi N (2005) Esterification reaction using solid heterogeneous acid catalysts under solvent-less condition. J Mol Catal A 240:186–190

    CAS  Google Scholar 

  • Serajuddin ATM (1999) Solid dispersion of poorly water-soluble drugs: early promises, subsequent problems, and recent breakthroughs. J Pharm Sci 88:1058–1066

    CAS  Google Scholar 

  • Shahidi F, Yeo J (2016) Insoluble-bound phenolics in food. Molecules 21:1216

    Google Scholar 

  • Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144–158

    CAS  Google Scholar 

  • Tanamatayarat P (2016) Antityrosinase, antioxidative activities, and brine shrimp lethality of ethanolic extracts from Protium serratum (Wall. ex Colebr.) Engl. Asian Pac J Trop Biomed 6:1050–1055

    Google Scholar 

  • van Hoogevest P, Wendel A (2014) The use of natural and synthetic phospholipids as pharmaceutical excipients. Eur J Lipid Sci Technol 116:1088–1107

    CAS  Google Scholar 

  • Varvara M, Bozzo G, Celano G, Disanto C, Pagliarone CN, Celano GV (2016) The use of ascorbic acid as a food additive: technical-legal issues. Ital J Food Saf 5(1):4313

    Google Scholar 

  • Verreck G (2012) The influence of plasticizers in hot-melt extrusion. In: Hot-Melt Extrusion: Pharmaceutical Applications, pp 93–112. https://doi.org/10.1002/9780470711415.ch5

  • Wang B, Zhang Z, Chang K, Cui J, Rosenkranz A, Yu J, Lin C-T, Chen G, Zang K, Luo J (2018) New deformation-induced nanostructure in silicon. Nano Lett 18:4611–4617

    CAS  Google Scholar 

  • White BL, Howard LR, Prior RL (2009) Polyphenolic composition and antioxidant capacity of extruded cranberry pomace. J Agric Food Chem 58:4037–4042

    Google Scholar 

  • Wong YH, Lau HW, Tan CP, Long K, Nyam KL (2014) Binary solvent extraction system and extraction time effects on phenolic antioxidants from kenaf seeds (Hibiscus cannabinus L.) extracted by a pulsed ultrasonic-assisted extraction. Sci World J 2014:1–8

    Google Scholar 

  • Yamul DK, Lupano CE (2005) Whey protein concentrate gels with honey and wheat flour. Food Res Int 38:511–522

    CAS  Google Scholar 

  • Yoon S-D (2013) Cross-linked potato starch-based blend films using ascorbic acid as a plasticizer. J Agric Food Chem 62:1755–1764

    Google Scholar 

  • Yu L, Haley S, Perret J, Harris M (2002) Antioxidant properties of hard winter wheat extracts. Food Chem 78:457–461

    CAS  Google Scholar 

  • Zawawi N, Gangadharan P, Zaini RA, Samsudin MG, Karim R, Maznah I (2014) Nutritional values and cooking quality of defatted Kenaf seeds yellow (DKSY) noodles. Int Food Res J 21(2):603–608

    Google Scholar 

  • Zhang Z, Huo F, Zhang X, Guo D (2012a) Fabrication and size prediction of crystalline nanoparticles of silicon induced by nanogrinding with ultrafine diamond grits. Scr Mater 67:657–660

    CAS  Google Scholar 

  • Zhang Z, Song Y, Xu C, Guo D (2012b) A novel model for undeformed nanometer chips of soft-brittle HgCdTe films induced by ultrafine diamond grits. Scr Mater 67:197–200

    CAS  Google Scholar 

  • Zhang Z, Huo Y, Guo D (2013) A model for nanogrinding based on direct evidence of ground chips of silicon wafers. Sci China Technol Sci 56:2099–2108

    CAS  Google Scholar 

  • Zhang Z, Guo D, Wang B, Kang R, Zhang B (2015a) A novel approach of high speed scratching on silicon wafers at nanoscale depths of cut. Sci Rep 5:16395

    CAS  Google Scholar 

  • Zhang Z, Wang B, Kang R, Zhang B, Guo D (2015b) Changes in surface layer of silicon wafers from diamond scratching. CIRP Ann 64:349–352

    Google Scholar 

  • Zhang Z, Wang B, Zhou P, Guo D, Kang R, Zhang B (2016a) A novel approach of chemical mechanical polishing using environment-friendly slurry for mercury cadmium telluride semiconductors. Sci Rep 6:22466

    CAS  Google Scholar 

  • Zhang Z, Wang B, Zhou P, Kang R, Zhang B, Guo D (2016b) A novel approach of chemical mechanical polishing for cadmium zinc telluride wafers. Sci Rep 6:26891

    CAS  Google Scholar 

  • Zhang Z, Cui J, Wang B, Wang Z, Kang R, Guo D (2017) A novel approach of mechanical chemical grinding. J Alloys Compd 726:514–524

    CAS  Google Scholar 

  • Zhang Z, Shi Z, Du Y, Yu Z, Guo L, Guo D (2018) A novel approach of chemical mechanical polishing for a titanium alloy using an environment-friendly slurry. Appl Surf Sci 427:409–415

    CAS  Google Scholar 

  • Zhang Z, Cui J, Zhang J, Liu D, Yu Z, Guo D (2019) Environment friendly chemical mechanical polishing of copper. Appl Surf Sci 467:5–11

    Google Scholar 

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Acknowledgements

This study was supported by the Kangwon National University research Grant 2017 (520170420). We also highly acknowledge the Institute of Kenaf Co., Ltd., Chuncheon 24341, Republic of Korea.

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Authors and Affiliations

  1. Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University, 1 Kangwon daehakgil, Chuncheon-si, Kangwon-do, 24341, Republic of Korea

    Md Adnan, Md Obyedul Kalam Azad, Hyeon Seok Ju, Ji Min Son, Cheol Ho Park, Myung Hwan Shin & Dong Ha Cho

  2. Institute of Kenaf Co., Ltd., Chuncheon, 24341, Republic of Korea

    Md Adnan, Md Obyedul Kalam Azad, Hyeon Seok Ju, Ji Min Son & Dong Ha Cho

  3. Department of Medical Biomaterials Engineering, College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2 dong, Chuncheon, Kangwon-do, 200-701, Republic of Korea

    Madhusudhan Alle

  4. SJ Global LTD, Gyeongsangnam do, Busan, 635-890, Republic of Korea

    Myung Hwan Shin

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  1. Md Adnan
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Contributions

MA designed and conducted the experiment, analyzed the data, and drafted the final manuscript, MOKA, JMS, HSJ, AM, CHP, and MHS revised and improved the manuscript, DHC supervised this research.

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Correspondence to Madhusudhan Alle or Dong Ha Cho.

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Adnan, M., Azad, M.O.K., Ju, H.S. et al. Development of biopolymer-mediated nanocomposites using hot-melt extrusion to enhance the bio-accessibility and antioxidant capacity of kenaf seed flour. Appl Nanosci 10, 1305–1317 (2020). https://doi.org/10.1007/s13204-019-01205-z

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