Abstract
Antibiotic resistance is one of the most important public health concerns worldwide. Essential oils especially as micro- and nanoemulsion formulations are emerging as potential agents to target resistant pathogens and as drug delivery vehicles. Cinnamon and clove oils were formulated into nanoemulsions using Brij 35 or Tween 20. Based on the physicochemical characteristics and stability, CiB-3, ClB-3, CiT20-5 and ClT20-5 were identified as optimised emulsions and their anti-Staphylococcal properties were determined. Hydrodynamic diameter of the oil droplets was < 15 nm. The emulsions have only 5% of oil, and there was an 83–166-fold improvement in static MIC over the bulk oil. Inocula ranging from 105 to 107 CFU mL−1 of both MSSA and VISA strains did not significantly affect the activity of all four nanoemulsions. Tween 20 containing nanoemulsions had higher anti-biofilm activity (66–76%) and the activity was comparable between MSSA and VISA. ClT20-5 decreased the viable counts of both strains below detectable limits by 24 h. There was a statistically significant difference in leakage of 260-nm-absorbing cellular components between MSSA and VISA at 30 min (CiB-3: P = 4.67e−013, ClB-3: P = 2.49e−010, CiT20-5: P = 0.00028, ClT20-5: P = 0.00056) and at 60 min (CiB-3: P = 1.37e−014, ClB-3: P = 4.14e−014, CiT20-5: P = 5.30e−012, ClT20-5: P = 2.82e−009). The SEM images of the treated and untreated cells show significant damage and alteration in the morphology of the emulsion treated cells. This study demonstrates the significant anti-Staphylococcal activity of cinnamon and clove oil against MSSA and VISA strains.
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References
Al-Adham I, Al-Nawajeh A, Khalil E, Collier P (2012) The antimicrobial activity of oil-in-water microemulsions is predicted by their position within the microemulsion stability zone. Int Arab J Antimicrob Agents 2:1–8. https://doi.org/10.3823/711
Azeem A, Rizwan M, Ahmad FJ, Iqbal Z, Khar RK, Aqil M, Talegaonkar S (2009) Nanoemulsion components screening and selection: a technical note. AAPS Pharmscitech 10:69–76. https://doi.org/10.1208/s12249-008-9178-x
Bauer K, Garbe D, Surburg H (2006) Common fragrance and flavour materials. Preparation, properties and uses. IV Compl. Revised edition. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Carson CF, Mee BJ, Riley TV (2002) Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assays and electron microscopy. Antimicrob Agents Chemother 46:1914–1920. https://doi.org/10.1128/aac.46.6.1914-1920.2002
Carvalho IT, Estevinho BN, Santos L (2016) Application of microencapsulated essential oils in cosmetic and personal healthcare products—a review. Int J Cosmetic Sci 38:109–119. https://doi.org/10.1111/ics.12232
Costerton JW, Stewart PS, Greenberg EP (1999) Bacterial biofilms: a common cause of persistent infections. Science 284:1318–1322
Crossley KB, Jefferson KK, Archer GL, Fowler VG (2009) Staphylococci in human disease. Wiley, New York
David B, Wolfender J-L, Dias DA (2015) The pharmaceutical industry and natural products: historical status and new trends. Phytochem Rev 14:299–315. https://doi.org/10.1007/s11101-014-9367-z
Devi Y, Punithavathy P, Thomas S, Veeraraghavan B (2015) Challenges in the laboratory diagnosis and clinical management of heteroresistant vancomycin Staphylococcus aureus (hVISA). Clin Microbiol Open Access. https://doi.org/10.4172/2327-5073.1000214
Franklyne JS, Mukherjee A, Chandrasekaran N (2016) Essential oil micro and nano emulsions: promising roles in antimicrobial therapy targeting human pathogens. Lett Appl Microbiol. https://doi.org/10.1111/lam.12631
Gelbrand H et al (2015) The state of the World’s antibiotics 2015. Wound Heal South Afr 8:30–34
Ghosh V, Saranya S, Mukherjee A, Chandrasekaran N (2013) Cinnamon oil nanoemulsion formulation by ultrasonic emulsification: investigation of its bactericidal activity. J Nanosci Nanotechnol 13:114–122. https://doi.org/10.1166/jnn.2013.6701
Goud R, Gupta S, Neogi U, Agarwal D, Naidu K, Chalannavar R, Subhaschandra G (2011) Community prevalence of methicillin and vancomycin resistant Staphylococcus aureus in and around Bangalore, southern India. Revista da Sociedade Brasileira de Medicina Tropical 44:309–312. https://doi.org/10.1590/S0037-86822011005000035
Guenther E (1948) The essential oils, vol 6. D. van Nostrand Company Inc., New York
Howden BP (2005) Recognition and management of infections caused by vancomycin-intermediate Staphylococcus aureus (VISA) and heterogenous VISA (hVISA). Intern Med J. https://doi.org/10.1111/j.1444-0903.2005.00986.x
Hubálek Z (2003) Protectants used in the cryopreservation of microorganisms. Cryobiology 46:205–229. https://doi.org/10.1016/S0011-2240(03)00046-4
Hugo W, Longworth A (1964) Some aspects of the mode of action of chlorhexidine. J Pharm Pharmacol 16:655–662. https://doi.org/10.1111/j.2042-7158.1964.tb07384.x
Hyldgaard M, Mygind T, Meyer RL (2012) Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Front Microbiol. https://doi.org/10.3389/fmicb.2012.00012
Jevons MP (1961) “Celbenin”-resistant staphylococci. Br Med J 1:124
Lee D-G, Murakami Y, Andes DR, Craig WA (2013) Inoculum effects of ceftobiprole, daptomycin, linezolid, and vancomycin with Staphylococcus aureus and Streptococcus pneumoniae at inocula of 105 and 107 CFU injected into opposite thighs of neutropenic mice. Antimicrob Agents Chemother 57:1434–1441. https://doi.org/10.1128/aac.00362-12
Limbago BM, Kallen AJ, Zhu W, Eggers P, McDougal LK, Albrecht VS (2014) Report of the 13th vancomycin-resistant Staphylococcus aureus isolate from the United States. J Clin Microbiol 52:998–1002. https://doi.org/10.1128/jcm.02187-13
Meng Y et al (2016) Multi-functional liposomes enhancing target and antibacterial immunity for antimicrobial and anti-biofilm against methicillin-resistant Staphylococcus aureus. Pharm Res 33:763–775. https://doi.org/10.1007/s11095-015-1825-9
Moghimi R, Ghaderi L, Rafati H, Aliahmadi A, McClements D (2016) Superior antibacterial activity of nanoemulsion of Thymus daenensis essential oil against E. coli. Food Chem. https://doi.org/10.1016/j.foodchem.2015.07.139
Moreau-Marquis S, Stanton BA, O’Toole GA (2008) Pseudomonas aeruginosa biofilm formation in the cystic fibrosis airway. Pulm Pharmacol Ther 21:595–599. https://doi.org/10.1016/j.pupt.2007.12.001
Nannini EC, Singh KV, Arias CA, Murray BE (2013) In vivo effects of cefazolin, daptomycin, and nafcillin in experimental endocarditis with a methicillin-susceptible Staphylococcus aureus strain showing an inoculum effect against cefazolin. Antimicrob Agents Chemother 57:4276–4281. https://doi.org/10.1128/aac.00856-13
Periasamy S et al (2012) How Staphylococcus aureus biofilms develop their characteristic structure. Proc Natl Acad Sci 109:1281–1286. https://doi.org/10.1073/pnas.1115006109
Rammelkamp CH, Maxon T (1942) Resistance of Staphylococcus aureus to the action of penicillin. Proc Soc Exp Biol Med 51:386–389. https://doi.org/10.3181/00379727-51-13986
Shen S, Zhang T, Yuan Y, Lin S, Xu J, Ye H (2015) Effects of cinnamaldehyde on Escherichia coli and Staphylococcus aureus membrane. Food Control 47:196–202. https://doi.org/10.1016/j.foodcont.2014.07.003
Sikkema J, Poolman B, Konings WN, de Bont JA (1992) Effects of the membrane action of tetralin on the functional and structural properties of artificial and bacterial membranes. J Bacteriol 174:2986–2992. https://doi.org/10.1128/jb.174.9.2986-2992.1992
Standards NCfCL (1999) Methods for determining bactericidal activity of antimicrobial agents: approved guideline. NCCLS, Wayne
Sugumar S, Nirmala J, Ghosh V, Anjali H, Mukherjee A, Chandrasekaran N (2013) Bio-based nanoemulsion formulation, characterization and antibacterial activity against food-borne pathogens. J Basic Microbiol 53:677–685
Syed HK, Peh K (2014) Identification of phases of various oil, surfactant/co-surfactants and water system by ternary phase diagram. Acta Pol Pharm 71:301
Teixeira PC, Leite GM, Domingues RJ, Silva J, Gibbs PA, Ferreira JP (2007) Antimicrobial effects of a microemulsion and a nanoemulsion on enteric and other pathogens and biofilms. Int J Food Microbiol 118:15–19. https://doi.org/10.1016/j.ijfoodmicro.2007.05.008
Tong SYC, Davis JS, Eichenberger E, Holland TL, Fowler VG (2015) Staphylococcus aureus Infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev 28:603–661. https://doi.org/10.1128/CMR.00134-14
Tongnuanchan P, Benjakul S (2014) Essential oils: extraction, bioactivities, and their uses for food preservation. J Food Sci 79:R1231–R1249. https://doi.org/10.1111/1750-3841.12492
Vetas D, Dimitropoulou E, Mitropoulou G, Kourkoutas Y, Giaouris E (2017) Disinfection efficiencies of sage and spearmint essential oils against planktonic and biofilm Staphylococcus aureus cells in comparison with sodium hypochlorite. Int J Food Microbiol 257:19–25. https://doi.org/10.1016/j.ijfoodmicro.2017.06.003
Wilson LG (1987) The early recognition of streptococci as causes of disease. Med Hist 31:403
Zhang H, Shen Y, Weng P, Zhao G, Feng F, Zheng X (2009) Antimicrobial activity of a food-grade fully dilutable microemulsion against Escherichia coli and Staphylococcus aureus. Int J Food Microbiol 135:211–215. https://doi.org/10.1016/j.ijfoodmicro.2009.08.015
Zhang H, Cui Y, Zhu S, Feng F, Zheng X (2010) Characterization and antimicrobial activity of a pharmaceutical microemulsion. Int J Pharm 395:154–160. https://doi.org/10.1016/j.ijpharm.2010.05.022
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Franklyne, J.S., Andrew Ebenazer, L., Mukherjee, A. et al. Cinnamon and clove oil nanoemulsions: novel therapeutic options against vancomycin intermediate susceptible Staphylococcus aureus. Appl Nanosci 9, 1405–1415 (2019). https://doi.org/10.1007/s13204-019-01111-4
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DOI: https://doi.org/10.1007/s13204-019-01111-4