Skip to main content
SpringerLink
Log in

Influence of salt stress on inoculated Casuarina glauca seedlings

  • Published:
Symbiosis Aims and scope Submit manuscript

Abstract

Salinity is a serious threat to agriculture in arid and semi-arid regions including Egypt. There is currently a need to select highly salt-tolerant plants to improve recycling of agricultural drainage waters. Due to the importance of Casuarina in soil reclamation and rehabilitation, our study was carried out to evaluate the survival and growth of inoculated Casuarina glauca in hydroponic N-free medium supplemented with different NaCl concentrations (50, 100, 200, 300, 400 and 500 mM). Salt tolerant Frankia strains CcI156 and CgIM4, isolated from root nodules of Casuarina trees grown in loamy sand soils in Egypt, were used for seedling inoculation. Our results showed that inoculated Casuarina seedlings were able to withstand up to 200 mM NaCl. At higher NaCl concentrations (300 to 500 mM), the seedling died gradually three days after exposure to salt stress. However, at lower NaCl (50 mM) concentration, inoculated C. glauca seedlings showed a higher growth rate and higher percentages of nodulation. Nitrogenase activity and the total nitrogen content of nodulated seedlings were also influenced by elevated NaCl concentrations and recorded a reduction at high concentration (200 mM). Salt stress had a strong effect on biosynthesis of osmoprotectants molecules like L-Proline and carbohydrates, which strongly correlated (r = 0.98 and 0.87, respectively) with increasing salt concentrations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Abdul-Qados AMS (2011) Effect of salt stress on plant growth and metabolism of bean plant Vicia faba (L.). J Saudi Soc Agric Sci 10(1):7–15

    CAS  Google Scholar 

  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Physiol 39:205–207

    CAS  Google Scholar 

  • Batista-Santos P, Duro N, Rodrigues AP, Semedo JN, Alves P, da Costa M, Graça I, Pais IP, Scotti-Campos P, Lidon FC, Leitão AE, Pawlowski K, Ribeiro-Barros AI, Ramalho JC (2015) Is salt stress tolerance in Casuarina glauca Sieb. Ex Spreng. Associated with its nitrogen-fixing root-nodule symbiosis: an analysis at the photosynthetic level. Plant Physiol Biochem 96:97–109

    Article  CAS  PubMed  Google Scholar 

  • Bogusz D, Bonneau J, Franche C (2015) Transgenic approaches to study valuable genes in Casuarinaceae trees: a review. NFT News 12(1):4–7

    Google Scholar 

  • Bolanos LL, Martı M, El-Hamdaoui A, Rivilla R, Bonilla I (2006) Nitrogenase inhibition in nodules from pea plants grown under salt stress occurs at the physiological level and can be alleviated by B and Ca. Plant Soil 280:135–142

    Article  CAS  Google Scholar 

  • Chaves MM, Flexas J, Pinheiro C (2008) Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Ann Bot 103:551–560

    Article  PubMed  PubMed Central  Google Scholar 

  • Dawson JO, Gibson AH (1987) Sensitivity of selected Frankia isolates from Casuarina, Allocasuarina and north American host plants to sodium chloride. Physiol Plant 70(2):272–278

    Article  CAS  Google Scholar 

  • de Oliveira AB, Lídia N, Alencar M, Gomes-Filho E (2013) Comparison between the water and salt stress effects on plant growth and development. In: Sener A, (eds) Responses of Organisms to Water Stress, InTech; pp 67–94. doi:10.5772/54223

  • Delgado MJ, Ligero F, Lluch C (1994) Effects of salt stress on growth and nitrogen fixation by pea, faba-bean, common bean and soybean plants. Soil Biol Biochem 26(3):371–376

    Article  CAS  Google Scholar 

  • Devkota M, Martius C, Gupta RK, Devkota KP, McDonald AJ, Lamers JPA (2015) Managing soil salinity with permanent bed planting in irrigated production systems in Central Asia agriculture. Ecosyst Environ 202:90–97

    Article  CAS  Google Scholar 

  • Diagne N, Arumugam K, Ngom M, Nambiar-Veetil M, Franche C, Narayanan KK, Laplaze L (2013) Use of Frankia and actinorhizal plants for degraded lands reclamation. Bio Med Research International 948258, 9 pages. doi:10.1155/2013/948258

  • Diem HG, Dommergues YR (1990) Current and potential uses and management of Casuarinaceae in the tropics and subtropics. In: Schwintzer R, Tjepkema JD (eds) The biology of Frankia and actinorhizal plants. Academic Press, London, pp. 317–342

    Chapter  Google Scholar 

  • Dupont L, Alloing G, Pierre O, El Msehli S, Hopkins J, Hérouart D, Frendo P (2012) The legume root nodule: from symbiotic nitrogen fixation to senescence. In: Nagata T. (ed) Senescence, InTech Croatia, pp 137–168. doi:10.5772/34438

  • El-Lakany MH (1983) A review of breeding drought resistant Casuarina for shelterbelt establishment in arid regions with special reference to Egypt. For Ecol Manag 6:129–137. doi:10.1016/0378-1127(83)90017-8

    Article  Google Scholar 

  • El-Lakany MH (1986) Contribution of Casuarina to soil fertility in Egypt, in nitrogen fixing trees workshop: biological improvement of soil, Dakar

  • El-Lakany MH, Luard EJ (1982) Comparative salt tolerance of selected Casuarina species. Austalian Forestry Research 13:11–20

    Google Scholar 

  • Eswaran H, Lal R, Reich PF (2001) Land degradation: an overview. In: Bridges EM, Hannam ID, Oldeman LR, de Vries Pening FWT, Scherr SJ, Sompatpanit S (eds) Responses to land degradation, pro c.2nd.international conference on land degradation and desertification, Khon Kaen, Thailand. Oxford Press, New Delhi

    Google Scholar 

  • FAO (1995) Proceedings of the Regional workshop on management of salt-affected soils in the Arab Gulf States Abu-Dhabi, United Arab Emirates 29 October - 2 November

  • Franche C, Lindström K, Elmerich C (2009) Nitrogen-fixing bacteria associated with leguminous and non-leguminous plants. Plant Soil 321:35–59. doi:10.1007/s11104-008-9833-8

    Article  CAS  Google Scholar 

  • Geetanjali M, Neera G (2008) Salinity and its effects on the functional biology of legumes. Acta Physiol Plant 30(5):595–618

    Article  Google Scholar 

  • Hedge JE, Hofreiter BT (1962) Carbohydrate chemistry 17. In: Whistler RL, Miller JN (eds) Academic Press, New York.

  • Hoagland R, Arnon DI (1950) The water-culture method for growing plants without soil. California Agr Exp Sta Circ 347:36–39

    Google Scholar 

  • ICARDA (2011) Technical paper based on the Egypt-Australia-ICARDA Workshop on On-farm Water-use Efficiency, July 2011, Cairo-Egypt

  • Mansour SR, Megahed MM (2002) Interaction of soil and different Frankia strains on nodulation and mass production of three Casuarina species. Egypt J Microbiol 37(4):332

    Google Scholar 

  • Mansour SR, Torrey JG (1991) Frankia spores of strain HFPCgI4 as inoculum for seedlings of Casuarina glauca. Can J Bot 69:1251–1256

    Article  Google Scholar 

  • Mansour SR, Dewedar A, Torrey JG (1990) Isolation, culture and behavior of Frankia strain HFPCgI4 from root nodules of Casuarina glauca. Bot Gaz 151:490

    Article  Google Scholar 

  • Mansour SR, Zayed A, Safwat M (2002) Field trial for measuring N2 fixation efficiency of different inoculum treatments of Casuarina species using 15N-labelling method. Egypt J Microbiol 37(4):409

    Google Scholar 

  • Masaa EV, Nieman RH (1978) Physiology of plant tolerance in salinity. In: Crop tolerance to suboptimal land conditions. American Society of Agronomy Special Publication, USA, pp. 277–299

  • Murry A, Fontaine MS, Torrey JG (1984) Growth kinetics and nitrogenase induction in Frankia sp. HFPArI3 grown in batch culture. Plant Soil 78:61–78

    Article  CAS  Google Scholar 

  • Reddell P, Foster RC, Bowen GD (1986) The effects of sodium chloride on growth and nitrogen fixation in Casuarina obesa Miq. New Phytol 102:397–408

    Article  CAS  Google Scholar 

  • Serraj R, Roy G, Drevon JJ (1994) Salt stress induces a decrease in the oxygen uptake of soybean nodules and in their permeability to oxygen diffusion. Physiol Plant 91:161–168

    Article  CAS  Google Scholar 

  • Tani C, Sasakawa H (2000) Salt tolerance of Elaeagnus macrophylla and Frankia Ema1 strain isolated from the root nodules of E. macrophylla. Soil Sci Plant Nutr 46(4):927–937. doi:10.1080/00380768.2000.10409158

    Article  Google Scholar 

  • Tani C, Sasakawa H (2003) Salt tolerance of Casuarina equisetifolia and Frankia Ceq1 strain isolated from the root nodules of C. equisetifolia. Soil Sci Plant Nutr 49(2):215–222. doi:10.1080/00380768.2003.10410000

    Article  Google Scholar 

  • Tani C, Sasakawa H (2006) Proline accumulates in Casuarina equisetifolia seedlings under salt stress. Soil Sci Plant Nutr 52:21–25

    Article  CAS  Google Scholar 

  • Tu JC (1981) Effect of salinity on Rhizobium-root hair interaction nodulation and growth of soybean. Can J Plant Sci 61:231–239

    Article  Google Scholar 

  • Vijayvargiya S, Kumar A (2011) Influence of salinity stress on plant growth and productivity: salinity stress influences on plant growth, Germany. Lap Lambert Academic Publishers, pp170

  • Yancey PH, Clark ME, Hand SC, Bowlus RD, Somero GN (1982) Living with water stress: evolution of osmolyte systems. Science 217:1214–1222

    Article  CAS  PubMed  Google Scholar 

  • Zharan HH, Sprent JI (1986) Effects of sodium chloride and polyethylene glycol on root-hair infection and nodulation of Vicia faba L. Plants by Rhizobium leguminosarum. Planta 167:303–309

    Article  Google Scholar 

  • Zhong C, Mansour S, Mathish N, Bougusz D, Franche C (2013) Casuarina glauca: a model tree for basic research in actinorhizal symbiosis. J Biosci 38(4):815–823

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the Science and Technology Development Fund (STDF), grant No. 900, as International Cooperation Project. The authors express their appreciation to Prof. Louis Tisa for revising the manuscript. Authors also express their thanks to Prof. Philippe Normand and the anonymous referees for their valuable and constructive notes.

Author information

Authors and Affiliations

  1. Botany Department, Faculty of Science, Suez Canal University, Ismailia, Egypt

    Samira R. Mansour

  2. Genetics Department, Faculty of Agriculture, Menoufia University, Shebeen El-Kom, Menoufia Governorate, Egypt

    Khalid Abdel-lateif

  3. Equipe Rhizogenèse, UMR DIADE, IRD (Institute de Recherche pour le Développement), BP 64501, 34394, Montpellier Cedex 5, France

    Didier Bogusz & Claudine Franche

Authors
  1. Samira R. Mansour
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Khalid Abdel-lateif
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Didier Bogusz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Claudine Franche
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Samira R. Mansour.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mansour, S.R., Abdel-lateif, K., Bogusz, D. et al. Influence of salt stress on inoculated Casuarina glauca seedlings. Symbiosis 70, 129–138 (2016). https://doi.org/10.1007/s13199-016-0425-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13199-016-0425-8

Keywords

Search

Navigation