Abstract
The present study aims to explore the outcome of processing methods such as fermentation; treatments using lemon juice (T1), sodium chloride (T2), lemon juice followed by sodium chloride (T3) and fermentation followed by treatment-T3 on the quality characteristics of processed finger millet flour to develop a specialized low protein food supplement for a protein-related inborn error of metabolic disorders. The clean dirt-free finger millet grains were made into slurry subjected to treatment T1 (FMFT1), T2 (FMFT2), T3 (FMFT3), fermentation for 8–36 h with 4 h intervals using yoghurt as starter culture (FFMF) and fermentation (8–36 h) followed by treatment T3 (FFMFT3). The acidity of the finger millet slurry significantly increased with the increase in fermentation time when compared to control. The IVPD was found to be 89% in FFMF (20 h) sample which was significantly higher than the IVPD of control sample flour (27%). However, the crude protein content (%), the protein fractions and the IVPD of FFMFT3 (8–36 h) samples were found to be lesser than the FFMF (8–36 h) samples. The findings were further ensured by the results of scanning electron microscopic images and FT-IR spectra which showed the morphological and chemical modifications caused by the processing methods. The setback and breakdown viscosity of control (945 cP and 664 cP respectively) approximately reduced to two-third in FFMF (20 h) samples and one-third for FFMFT3 samples (303 cP and 286 cP respectively). From the study, it is evident that the processing method of fermentation followed by treatment-T3 could be utilized in the development of low protein food supplements.
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References
Adebiyi JA, Obadina AO, Adebo OA, Kayitesi E (2018) Fermented and malted millet products in Africa: expedition from traditional/ethnic foods to industrial value-added products. Crit Rev Food Sci Nutr 58:463–474
Adgidzi EA, Ani JC, Karim R, Ghazali HM (2018) Physical and chemical characteristics of depigmented oven dried dehulled millet flours. Turk J Agricu Food Sci Technol 6:1022–1029
Afify AEMM, El-Beltagi HS, El-Salam SMA, Omran AA (2012) Protein solubility, digestibility and fractionation after germination of sorghum varieties. PLoS ONE. https://doi.org/10.1371/journal.pone.0031154.t001
Ali NM, El Tinay AH, Elkhalifa AEO, Salih OA, Yousif NE (2009) Effect of alkaline pretreatment and cooking on protein fractions of a high-tannin sorghum cultivar. Food Chem 114:649–651
Antony U, Chandra TS (1998) Antinutrient reduction and enhancement in protein, starch, and mineral availability in fermented flour of finger millet (Eleusine coracana). J Agric Food Chem 46:2578–2582
Antony U, Sripriya G, Chandra TS (1996) Effect of fermentation on the primary nutrients in finger millet (Eleusine coracana). J Agric Food Chem 44:2616–2618
AOAC (2000) Association of official agricultural chemists, 17edn. Official Methods of Analysis of AOAC International, Gaithersburg, MD
Bandekar J (1992) Amide modes and protein conformation. Biochem Biophys Acta 1120:123–143
Chandra D, Chandra S, Pallavi SAK (2016) Review of Finger millet (Eleusine coracana (L.) Gaertn): a power house of health benefiting nutrients. Food Sci Hum Wellness 5:149–155
Christopher R, Natarajan A (2016) Screening for inborn errors of metabolism by tandem mass spectrometry: an eight-year experience. Techagappe 2:21–23
Coates J (2006) Interpretation of infrared spectra, a practical approach. In: Meyers RA, McKelvy MS (eds) Encyclopedia of Analytical Chemistry. John Wiley & Sons Ltd, Chicester, England, pp 1–23. https://doi.org/10.1002/9780470027318.a5606
Duodu KG, Tang H, Grant T, Wellner N, Belton PS, Taylor JRN (2001) FTIR and solid state 13C NMR spectroscopy of proteins of wet cooked and popped sorghum and maize. J Cereal Sci 33:261–269
El hag ME, El tinay AH, Yousif NE (2002) Effect of fermentation and dehulling on starch, total polyphenol, phytic acid content and in-vitro protein digestibility of pearl millet. Food Chem 77:193–196
Gull A, Prasad K, Kumar P (2016) Evaluation of functional, antinutritional, pasting and microstructural properties of Millet flours. Food Meas Charact 10:96–102
Holliday MA, Anderson AS, Barness LA, Justice P (1976) Special diets for infants with inborn errors of amino acid metabolism. Pediatrics 57:783–792
Kapoor S, Thelma BK (2018) Status of newborn screening and inborn errors of metabolism in India. Indian J Pediatrics 85:1110–1117
Landry J, Moureaux T (1970) Heterogeneity of corn seed glutelin: selective extraction and amino acid composition of the 3 isolated fractions. Bulletin de la Societe de Chimie Biologique 52:1021–1037
Lansakara LHMPR, Liyanage R, Perera KA, Wijewardana I, Jayawardena BC, Vidanarachchi JK (2016) Nutritional composition and health-related functional properties of Eleusine coracana (Finger millet). Proc Food Scie 6:344–347
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin Phenol reagent. J Biol Chem 193:265–275
Lu ZH, Li LT, Min WH, Wang F, Tatsumi E (2005) The effects of natural fermentation on the physical properties of rice flour and the rheological characteristics of rice noodles. Int J Food Sci Technol 40:985–992
Mohiedeen IE, Tinay AHE, Elkhalya AEO, Babiker EE, Mallasiy LO (2010) Effect of fermentation on in-vitro protein digestibility, protein fractions and amino acids composition of maize (Zea mays Linnaeus) cultivars. Electron J Environ J Agric Food Chem 9:838–847
Nagaraja D, Mamatha SN, De T, Christopher R (2010) Screening for inborn errors of metabolism using automated electrospray tandem mass spectrometry: study in high-risk Indian population. Clin Biochem 43:581–588
Ndamitso MM, Abulude FO (2014) Effect of different salt concentrations on protein solubility of mushroom varieties obtained in Akure, Nigeria. Am J Food Nutr 2:7–10
Ogodo AC, Ugbogu OC, Onyeagba RA, Okereke HC (2018) Proximate composition and in-vitro starch/protein digestibility of Bambara Groundnut Flour fermented with lactic acid bacteria (LAB)-consortium isolate from cereals. Fermentation Technol 7:1–9
Palma-Rodriguez HM, Agama-Acevedo E, Mendez-Montealvo G, Gonzalez-Soto RA, Vernon-Carter EJ, Bello-Pérez LA (2012) Effect of acid treatment on the physicochemical and structural characteristics of starches from different botanical sources. Starch 64:115–125
Patel I, Patel K, Pinto S, Patel S (2016) Ragi: a powerhouse of nutrients. Res Rev J Dairy Sci Technol 5:36–47
Pena MJ, Almeida MF, Van Dam E, Ahring K, Belanger-Quintana A, Dokoupil K, Rocha JC (2015) Special low protein foods for phenylketonuria: availability in Europe and an examination of their nutritional profile. Orphanet J Rare Dis 10:162
Reddy CK, Suriya M, Haripriya S (2013) Physico-chemical and functional properties of Resistant starch prepared from red kidney beans (Phaseolus vulgaris. L) starch by enzymatic method. Carbohyd Polym 95:220–226
Sadasivam A, Manickam A (2018) Biochemical Methods, 3rd edn. New age International (P) Ltd. Publishers, New Delhi
Sade FO (2009) Proximate, antinutritional factors and functional properties of processed peral millet (Pennisetum glaucum). J Food Technol 7:92–97
Singh N, David J, Thompkinson DK, Seelam BS, Rajput H, Morya S (2018) Effect of roasting on functional and phytochemical constituents of finger millet (Eleusine coracana L.). Pharma Innov J 7:414–418
Sripriya G, Antony U, Chandra TS (1997) Changes in carbohydrate, free amino acids, organic acids, phytate and HCl extractability of minerals during germination and fermentation of finger millet (Eleusine coracana). Food Chem 58:345–350
Sun Q, Gong M, Li Y, Xiong L (2014) Effect of dry heat treatment on the physicochemical properties and structure of Proso millet flour and starch. Carbohyd Polym 110:128–134
Sze-Tao KWC, Sathe SK (2000) Functional properties and in vitro digestibility of almond (Prunus dulics L.) protein isolate. Food Chem 69:153–160
Utta DP, Pradesh VE, Singh TK (2015) Chemical composition of finger millet of food and nutritional security. Int J Food Sci Microbiol 3:92–98
Yang Y, Tao WY (2008) Effect of lactic fermentation on FT-IR and Pasting properties of rice flour. Food Res Int 41:937–940
Yousif NE, El Tinay AH (2003) Effect of natural fermentation on protein fractions and in vitro protein digestibility of rice. Plant Foods Hum Nutr 58:1–8
Zhang F, Liu M, Mo F, Zhang M, Zheng J (2017) Effects of acid and salt solutions on the pasting rheology and texture of lotus root starch-Konjac glucomannan mixture. Polymer 9:1–14
Acknowledgement
The authors would like to acknowledge, the department of food science and technology, the CIF, Pondicherry University, Pondicherry, and GRI-SEM for providing the necessary instrumentation facilities for the study. The first author would like to acknowledge Dr K.V. Sunooj, Assistant professor, PU and Dr S.S. Vijayanchali, Assistant professor, GRI for their guidance and the University Grants Commission for providing financial support as UGC-NET (JRF).
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G, G., M, R. & Narayanasamy, S. Efficacy of fermentation parameters on protein quality and microstructural properties of processed finger millet flour. J Food Sci Technol 58, 3223–3234 (2021). https://doi.org/10.1007/s13197-020-04826-3
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DOI: https://doi.org/10.1007/s13197-020-04826-3