Skip to main content
Log in

Antagonistic activity of Thymus vulgaris extracts against Vibrio species isolated from seafoods

Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

This study examined the antibacterial activity of Thymus vulgaris on multiple antibiotic resistant (MAR) Vibrio parahaemolyticus and Vibrio fluvialis isolated from shrimps. The ethanol extract of T. vulgaris antibacterial properties was assessed using the agar diffusion method. Survival of test organisms in shrimp meat using different concentrations of T. vulgaris was done using standard method. The quantitative and qualitative phytochemical tests of T. vulgaris extract were determined. The ethanol extract had antimicrobial activities on the test organisms showing 20.00 ± 0.0 and 23.00 ± 0.0 mm zone of inhibition on V. parahaemolyticus and V. fluvialis respectively. T. vulgaris completely decreased microbial load of V. parahaemolyticus and V. fluvialis in 150 and 60 min, respectively. The phytochemical screening for the ethanol extract reported phenol, alkaloids, tannin, saponin, anthraquinone flavonoid and cardiac glycoside as 51.76, 26.60, 6.76, 54.33, 30.35 89.65 and 18.23 mg/100 g, respectively. This study reveals the antibacterial property of T. vulgaris on the MAR Vibrio species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  • Aboaba OO, Eze AR, Anabuike CL (2011) Antimicrobial activities of some Nigeria spices on some pathogens. Agric Biol J N Am 2(8):1187–1193

    Article  Google Scholar 

  • Akintobi OA, Onoh CC, Ogele JO, Idowu AA, Ojo OV, Okonko IO (2013) Antimicrobial activity of Zingiber officinale extract against some selected pathogenic bacteria. Nat Sci 11(1):7–15

    Google Scholar 

  • Benbelaïd F, Khadir A, Abdoune MA, Bendahou M (2013) Phytochemical screening and in vitro antimicrobial activity of Thymus lanceolatus Desf. from Algeria. Asian Pac J Trop Dis 3(6):454–459

    Article  Google Scholar 

  • Dorman HGD, Deans SG (2000) Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 88:308–316

    Article  CAS  Google Scholar 

  • Dunstan RA, Heinz E, Wijeyewickrema LC, Pike RN, Purcell AW, Evans TJ, Praszkier J, Robins-Browne RM, Strugnell RA, Korotkov KV, Lithgow T (2013) Assembly of the type II secretion system such as found in Vibrio cholera depends on the Novel. PLoS Pathog 9(1):303–309

    Article  Google Scholar 

  • Figueiredo AC, Barroso JG, Pedro LG, Salgueiro L, Miguel MG, Faleiro ML (2008) Portuguese Thymbra and Thymus species volatiles: chemical composition and biological activities. Curr Pharm Des 14(29):3120–3140

    Article  CAS  Google Scholar 

  • Girova T, Gochev V, Jirovetz L, Buchbauer G, Schmidt E, Stoyanova A (2010) Antimicrobial activity of essential oils from spices against psychrotrophic food spoilage microorganism. Biotechnol Biotechnol Equip 24:547–552

    Article  Google Scholar 

  • Gull I, Saeed M, Shaukat H, Aslam SM, Samra ZQ, Athar AM (2012) Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria. Ann Clin Microbiol Antimicrob 11:8. doi:10.1186/1476-0711-11-8

    Article  Google Scholar 

  • Harbone DB (2008) Phytochemical methods, a guide to modern techniques of plant analysis, 3rd edn. Springer, New Delhi

    Google Scholar 

  • Ismail MM, Essam TM, Mohamed AF, Mourad FE (2012) Screening for the antimicrobial activities of alcoholic and aqueous extracts of some common spices in Egypt. Int J Microbiol Res 3(3):200–207

    Google Scholar 

  • Issabeagloo E, Kermanizadeh P, Taghizadieh M, Forughi R (2012) Antimicrobial effects of rosemary (Rosmarinus officinalis L.) essential oils against Staphylococcus spp. Afr J Microbiol Res 6(23):5039–5042

    Google Scholar 

  • Kareem KT, Kareem SO, Adeyemo OJ, Egberongbe RK (2010) In vitro antimicrobial properties of Bridelia ferruginea on some clinical isolates. Agric Biol J N Am 1(3):416–420

    Article  Google Scholar 

  • Mailgaad M, Civille GV, Carr BT (1999) Sensory evaluation techniques. CRS, Boca Raton

    Google Scholar 

  • Masniyom P, Benjakul S, Visessanguan W (2005) Combination effect of phosphate and modified atmosphere on quality and shelf-life extension of refrigerated seabass slices. LWT Food Sci Technol 38:745–756

    Article  CAS  Google Scholar 

  • Melvin MJ, Jayachitra J, Vijayapriya M (2009) Antimicrobial activity of some common spices against certain human pathogens. J Med Plant Res 3(11):1134–1136

    Google Scholar 

  • Nowak A, Kalemba D, Piotrowska M, Czyżowska A (2013) Effects of thyme (Thymus vulgaris L.) and rosemary (Rosmarinus officinalis L.) essential oils on growth of Brochothrix thermosphacta. Afr J Microbiol Res 7(26):3396–3404

    Article  Google Scholar 

  • Nzeako BC, Al-Kharousi ZSN, Al-Mahrooqui Z (2006) Antimicrobial activities of clove and thyme extracts. Sult Qaboos Univ Med J 6(1):34–39

    Google Scholar 

  • Olayemi AB, Opaleye FI (1999) Antibiotic resistance among coliform bacteria isolated from hospital and urban waste waters. World J Microbiol Biotechnol 6:285–288

    Article  Google Scholar 

  • Oramadike C, Ogunbanwo ST (2014) Incidence of Vibrio species in seafood samples collected from Lagos Lagoon, Nigeria. Am J Food Sci Nutr 1:76–82

    Google Scholar 

  • Oramadike C, Ogunbanwo ST (2015) Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from seafoods in Lagos Lagoon Nigeria. Cogent Food Agric 1:1041349

    Google Scholar 

  • Poole K (2001) Overcoming antimicrobial resistance by targeting resistance mechanisms. J Pharm Pharmacol 53:283–284

    Article  CAS  Google Scholar 

  • Rabiey S, Hosseini H, Rezaei M (2014) Use Carum copticum essential oil for controlling the Listeria monocytogenes growth in fish model system. Braz J Microbiol 45(1):89–96

    Article  Google Scholar 

  • Radulovic NS, Blagojevic PD, Stojanovic-Radic ZZ, Stojanovic NM (2013) Antimicrobial plant metabolites: structural diversity and mechanism of action. Curr Med Chem 20(7):932–952

    CAS  Google Scholar 

  • Reis FBD, Souza VMD, Thomaz MRS, Fernandes LP, Oliveira WPD, Martinis ECP (2011) Use of carnobacterium maltaromaticum cultures and hydroalcoholic extract of Lippia sidoides Cham. against Listeria monocytogenes in fish model systems. Int J Food Microbiol 146:228–234

    Article  Google Scholar 

  • Rivas L, McDonnel MJ, Burgess CM, O’Brien M, Navarro-Villa A, Fanning S, Duffy G (2010) Inhibition of verocytotoxigenic Escherichia coli in model broth and rumen systems by carvacrol and thymol. Int J Food Microbiol 139:70–78

    Article  CAS  Google Scholar 

  • Rojas R, Bustamante B, Bauer J, Fernández I, Albán J, Lock O (2003) Antimicrobial activity of selected peruvian medicinal plants. J Ethnopharmacol 88(2–3):199–204

    Article  Google Scholar 

  • Schirmer BC, Langsrud S (2010) Evaluation of natural antimicrobials on typical meat spoilage bacteria and in vacuum-packed pork meat. J Food Sci 75:98–102

    Article  Google Scholar 

  • Snoussi M, Hajlaoui H, Noumi E, Usai D, Sechi L, Zanetti S, Bakhrouf A (2008) In vitro anti Vibrio spp. activity and chemical composition of some Tunisian aromatic plants. World J Microbiol Biotechnol 24:3071–3076

    Article  CAS  Google Scholar 

  • Szczepaniak S, Polanska M, Van Assche A, Moloney R, Willems KA (2011) The synergism of natural compounds in the pursuit of safe and healthier ford. J Ind Microbiol Biotechnol 38:215–220

    Article  CAS  Google Scholar 

  • Usman UZ, Usman HM, Mainasara AS (2015) Role of African spices against Escherichia coli isolated from potable water sample in Sokoto, Nigeria. Adv Med Plant Res 3(2):62–68

    CAS  Google Scholar 

Download references

Acknowledgements

We acknowledge the Nigerian Institute for Oceanography and Marine Research (NIOMR) for sponsoring this research. Special thanks to Dr. G.R. Akande, Executive Director NIOMR, for his strong support.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Chigozie Eunice Oramadike.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Oramadike, C.E., Ogunbanwo, S.T. Antagonistic activity of Thymus vulgaris extracts against Vibrio species isolated from seafoods. J Food Sci Technol 54, 1199–1205 (2017). https://doi.org/10.1007/s13197-017-2543-6

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-017-2543-6

Keywords

Navigation