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Physicochemical analysis of Psophocarpus tetragonolobus (L.) DC seeds with fatty acids and total lipids compositions

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Abstract

Psophocarpus tetragonolobus (L.) DC. is a tropical legume with potential nutritional properties. In present study, the physical properties and proximate composition of the seeds were evaluated. Besides, the physico-chemical properties of fatty oil from fully mature seeds were also studied. The fatty oil compositions of immature, mature and fully mature seeds were evaluated by GC-FID, GC/MS and 1H-NMR. The study revealed that, fatty oil from fully mature seeds contained high proportion of unsaturated fatty acids (75.5 %), whereas immature seeds contained higher percentage of saturated fatty acid (61.3 %). In addition, unsaponification matter (0.25 %) of fatty oil was identified as stigmasterol (66.4 %) and β-sitosterol (25.1 %). Total lipids of fully mature seeds were extracted and isolated as neutral, glyco- and phospholipids. Overall, the fatty oil of fully mature seeds was enriched with mono-unsaturated fatty acids (38.6 %) and poly-unsaturated fatty acids (36.9 %) without trans-fatty acids, thus meeting the edible oil standard.

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References

  • Anonymous (1975) The winged bean: A high protein crop for the tropics. National academy of sciences, Washington, DC

  • AOAC 962.09 (1995) Fiber (crude) in animal fat. In: Cunniff P (ed). In: AOAC official method of analysis, 16th edn. Virginia, USA

    Google Scholar 

  • AOAC 974.19 (1995) Halphen Test. In: Cunniff P (ed). In: AOAC official method of analysis, 16th edn. Virginia, USA

    Google Scholar 

  • ASTM D 3174–04 (2004) Standard method for ash in the analysis sample of coal and coke. American Society for Testing Materials, International, Philadelphia, PA.

  • Bodger D, Davis JB, Farmery D, Hammonds TW, Harper AJ, Harris RV, Hebb L, MacFarlane N, Shanks P, Southwell K (1982) An investigation of the extraction, refining and composition of oil from Winged bean (Psophocarpus tetragonolobus D.C.). J Am Oil Chem Soc 59:523–530

    Article  CAS  Google Scholar 

  • Cerny K, Kordylas M, Pospisil F, Svabensky O, Zajic B (1971) Nutritive value of the winged bean (Psophocarpus palustris Desv.). Br J Nutr 26:293–299

    Article  CAS  Google Scholar 

  • Critchfield FE (2007) Organic functional group analysis. Pergamon Press, Oxford, UK, pp 131–136. doi:10.1126/science.143.3602.123

    Google Scholar 

  • Ekpenyong TE, Borchers RL (1980) The fatty acid composition of the oil of the Winged bean (Psophocarpus tetragonolobus L.) seeds. J Am Oil Chem Soc 57:147–149

    Article  CAS  Google Scholar 

  • Garcia VV, Palmer JK (1979) Fatty acid composition of the oil of Winged beans. Psophocarpus totragonolobus (L) DC J Am Oil Chem Soc 56:931–932

    Article  CAS  Google Scholar 

  • Garnayak DK, Pradhan RC, Naik SN, Bhatnagar N (2008) Moisture-dependent physical properties of jatropha seed (Jatropha curcas L.). Ind crops prod 27:123–129

    Article  CAS  Google Scholar 

  • Hagerman AE, Butler IG (1981) The specificity of proanthrocyanidin protein implication. J Biol Chem 256:4494–4497

    CAS  Google Scholar 

  • IS: 548 (1986) Methods of Sampling and test for oils and fats (Sampling physical and chemical tests). In: Bureau of Indian Standards, Bahadur Shah Zafer Marg, New Delhi, India, pp 29–52.

  • Khor HT, Chan SL (1988) Changes of lipid classes and fatty acid composition in developing Psophocarpus tetragonolobus seeds. Phytochem 27:2041–2044

  • Mohanty CS, Verma S, Singh V, Khan S, Gaur P, Gupta P, Nizar MA, Dikshit N, Pattanayak R, Shukla A, Niranjan A, Sahu N, Behera SK, Rana TS (2013) Characterization of winged bean (Psophocarpus tetragonolobus (L.) DC.) based on molecular, chemical and physiological parameters. Am J Mol Bio 3:187–197

    Article  Google Scholar 

  • Mohsenin NN (1986) Physical properties of plant and animal materials. Gordon and Breach Science Publishers. USA, New York

    Google Scholar 

  • Patil VV, Galge RV, Thorat BN (2010) Extraction and purification of phosphatidylcholine from soybean lecithin. Sep Purif Technol 75:138–144

    Article  CAS  Google Scholar 

  • Pradhan RC, Meda V, Naik SN, Tabil L (2010) Physical properties of canadian grown flaxseed in relation to its processing. Int J Food Proper 13:732–743

    Article  Google Scholar 

  • Rout PK, Naik SN, Rao YR, Jadeja G, Maheshwari RC (2007) Extraction and composition of volatiles from Zanthoxylum rhesta: Comparison of subcritical CO2 and traditional processes. J Supercrit Fluids 42:334–341

    Article  CAS  Google Scholar 

  • Rout PK, Rao YR, Jena KS, Sahoo D, Ali S (2014) Safety evaluation of Simarouba glauca seed fat. J Food Sci Technol. doi:10.1007/s13197-012-0636-9

    Google Scholar 

  • Sacchi R, Patumi M, Fontanazza G, Barone P, Fiordiponti P, Mannina L, Rossi E, Segre AL (1996) A high-field 1H nuclear magnetic resonance study of the minor components in virgin olive oils. J Am Oil Chem Soc 73:747–758

    Article  CAS  Google Scholar 

  • Sahoo D, Jena KS, Rout PK, Rao YR (2003) Two stage solvent extraction of seeds of Hibuiscus abelmoschus Linn: Lipid and FA compositions. J Am Oil Chem Soc 80:209–211

    Article  CAS  Google Scholar 

  • Sharma V, Das L, Pradhan RC, Naik SN, Bhatnagar N, Kureel RS (2011) Physical properties of tung seed: An industrial oil yielding crop. Ind Crops Prod 33:440–444

    Article  Google Scholar 

  • Varriano-Marston E, Beleia A, Lai CC (1983) Structural characteristics and fatty acid composition of Phophocarpus tetragonolobus seeds. Ann Bot 51:631–640

    CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to Directors CSIR-NBRI and CSIR-CIMAP for providing the laboratory facilities for carrying out this work.

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Correspondence to Prasant Kumar Rout.

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Mohanty, C.S., Pradhan, R.C., Singh, V. et al. Physicochemical analysis of Psophocarpus tetragonolobus (L.) DC seeds with fatty acids and total lipids compositions. J Food Sci Technol 52, 3660–3670 (2015). https://doi.org/10.1007/s13197-014-1436-1

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  • DOI: https://doi.org/10.1007/s13197-014-1436-1

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