Abstract
Aegle marmelos (AM) leaves were extracted with methanol (ME), ethanol (EE), water (WE) and analyzed for antioxidant activities by DPPH radical scavenging method, reducing power and in vitro inhibition by Fenton’s reagent—induced oxidation of lipid system. Stability of extracts to pH (4, 7 and 9) and temperature (100 °C, 15 min.) was studied. The three extracts showed varying degree of efficacy in each assay in a dose dependent manner. The inhibition of MDA formation in Linseed oil by EE (47%) was significantly (P < 0.05) higher than WE (28%) and ME (23%) but less than α- Tocopherol (80%). WE showed maximum stability to high temperature. The antioxidant activity of EE at pH 4 was significantly higher (P < 0.05) compared with WE and ME. At pH 7, the antioxidant activity of all the three extracts remained unchanged. Data indicates that potential exists for the utilization of Aegle marmelos as a natural antioxidant.
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Arabshahi-Delouee S, Devi V, Urooj A (2007) Evaluation ofantioxidant activity of some plant extracts and their heat, pHand storage stability. Food Chem 100:1100–1105
Arabshahi-Delouee S, Urooj A (2007) Antioxidant properties of various solvent extracts of mulberry ( Morus indica L.) leaves. Food Chem 102:1233–1244
Arul V, Miyazaki S, Dhanajayan R (1997) Studies on the anti inflammatory and pyretic and analgesic properties of the leaves of AM corrhea. J Ethanopharmacology 57:29
Azizah AH, Nik Ruslawati NM, Sweetee T (1999) Extraction and characterization of antioxidant from cocoa by- products. Food Chem 64:199–202
Beutler E, Kelly BM (1963) The effect of sodium nitrate on RBC glutathione. Experienita 19:96–97
Bassiounny SS, Hassanien FR, El-Razik Ali F, El- Kayati S (1990) Efficiency of antioxidants from natural sources in bakery products. Food Chem 37:297–305
Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature 26:1199–1200
Castenmiller JJM, Linssen JPH, Heinonen IM, Hopia AL, Schwarz K, Hollmann PCH (2002) Antioxidant properties of differently processed spinach products. Nahrung Food 46:290–293
Chanda R, Ghosh A, Mitra T, Mohanty JP, Bhuyan N, Pawankar G (2008) Phytochemical and pharmacological activity of Aegle marmelos as a potential medicinal plant: An overview. The Internet J Pharm. 6(1)
Dhuley JN (2003) Investigation on the gastroprotective and antidiarrhoeal properties of Aegle marmelos unripe fruit extract. Hindustan Antibiot Bull 4:41–46
Dorman HJ, Kosar M, Kahlos K, Holm H, Hiltuner R (2003a) Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties and cultivars. J Agric Food Chem 51:4563–4567
Dorman HJD, Peltoketo A, Hiltunen R, Tikkanen MJ (2003b) Characterization of the antioxidant properties of de-odorized aqueous extracts from selected Lamiaceae herbs. Food Chem 83:255–262
Freed M (1966) Methods of vitamins assay (3rd ed) Association of Vitamin Chemists. Interscience, New York
Gazzani G, Papetti A, Massolini G, Daglia M (1998) Antioxidative and pro- oxidant activity of water soluble components of some common diet vegetables and the effect of thermal treatment. J Agric Food Chem 6:4118–4122
Kesari AN, Gupta RK, Singh SKR, Diwakar S, Watal G (2006) Hypoglycemic and antihyperglycemic activity of Aegle marmelos seed extract in normal and diabetic rats. J Ethno 107(3):11374–379
Khangan VW, Roul AL (1996) Binding of copper ion in Aegle marmelos. J Ind Pollut Control 12(2):97–108
Kaur C, Kapoor HC (2000) Antioxidants in fruits and vegetables—the millennium’s health. Intl J Food Sci Tech 36:703–725
Koleva IJ, Linssen JPH, Beek TAV, Evstatieva LN, Kortenska V, Handjieva N (2003) Antioxidant activity, screening of extracts from siderites species (Labiateae) grown in Bulgaria. J Sci Food Agric 83:809–819
Lampronti D, Martello N, Bianchi M, Borgatti E, Lambertini R, Piva S, Jabbar M (2003) In vitro antiproliferative effects on human tumor cell lines of extracts from the Bangladeshi medicinal plant Aegle marmelos Correa. Phytomedicine 10(4):300–308
Mansour EH, Khalil AH (2000) Evaluation of antioxidant activity of some plants extracts and their application to ground beef patties. Food Chem 69:135–141
Marzine PS, Gilbart R (2005) The effect of an aqueous extract of A. marmelos fruits on serum and tissue lipids in experimental diabetes. J Sci Food Agric 85(4):569–573
Miliauskas G, Venskutonis PR, Van Beek TA (2004) Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 85:231–237
Narender T, Shweta S, Tiwari P, Papi Reddy K, Khaliq T, Prathipati P, Puri A, Srivastava AK, Chander R, Agarwal SC, Raj K (2007) Antihyperglycemic and antidyslipidemic agent from Aegle marmelos. Bioorg Med Chem Lett 17(6):1808–1811
Ohkawa H, Ohoshi N, Yagi K (1994) Assay for lipid peroxides in animal tissues by thiobarbituric reaction. Anal Biochem 95:351–358
Porto CD, Calligaris S, Celloti E, Nicolim C (2000) Antiradical properties of commercial cognacs assessed by the DPPH test. J Agric Food Chem 48:1233–1241
Rana BK, Jain AK (1997) Evaluation of anti-fungal activity of essential oil isolated from leaves of the A marmelos. J Ethno 57(1):29–37
Ranganna S (1999) Handbook of Analysis and Quality Control for Fruits and Vegetables Products (2nd ed). McGrow-Hill, New Delhi, India
Reddy VP, Urooj A, Kumar A (2005) Evaluaiton of antioxidant activity of some medicina plant extracts and their application in biscuits. Food Chem 90:317–321
Reddy VP, Desai S, Ahmed F, Urooj A (2010) Antioxidant properties and stability of Raphanus sativus extracts. J Pharm Research 3(3):658–661
Riyanto SS, Mustafa AL (2000) Isolation of some chemical compounds from Aegle marmelos. Majalah Farmasi Indones 11(1):17–32
Roy LG, Delouee SA, Urooj A (2010) Antioxdiant efficacy of mulberry (Morus indica) leaves extract and powder in edible oil. Int J Food Prop 13:1–9
Sabu MC, Kuttan R (2004) Antidiabetic activity of Aegle marmelos and its relationship with its antioxidant properties. Indian J Physiol Pharmacol 48(1):81–88
Sachindra NM, Airanthi MKWA, Hosokawa M, Miyashita K (2010) Radical scavenging and singlet oxygen quenching activity of extracts from Indian seaweeds. J Food Sci Tech 47(1):94–99
Sakihama Y, Cohen MF, Grace SC, Yamasaki H (2002) Plant phenolic antioxidant and prooxidant activities: phenolics-induced oxidative damage mediated by metals in plants. Toxicology 177(1):67–80
Shoba FS, Thomas M (2001) Study of antidiarrheal activity of four medicinal plants in Castor oil induced diarrhea. J Ethano 76:73–76
Siddharaju P, Mohan PS, Becker K (2002) Studies on the antioxidant activity of Indian Laburnum (Cassia fistula) a preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp. Food Chem 79:61–67
Slinkard K, Singleton VL (1967) Total Phenol Analysis; Automation and comparison with manual methods. Am J Enology Viticulture 28:49–55
Takeoaka GR, Dao LT, (2003) Antioxidant constituents of almond [Pinus dulcis (Mill.) D.A. Webb] hulls. J Agric Food Chem 496–501
Tamura H, Yamagami A (1994) Antioxidant activity of monoacylated anthocyanins isolated from Muscat. Bailay A grape. J Agric Food Chem 42:1612–1616
Upadhyay S, Shanbhag KK, Suneetha G, Naidu BM, Upadhya S (2004) A study of hypoglycemic and antioxidant activity of Aegle marmelos in alloxan induced diabetic rats. Indian J Physio Pharmocol 48(4):476–480
Yen GC, Duh PD, Tsai CL (1993) Relationship between antioxidant activity and maturity of peanut hulls. J Agric Food Chem 41:67–70
Yildirim A, Mavi A, Kara AA (2003) Antioxidant and antimicrobial activities of Polygonum cognatum Meissn extracts. J Sci Food Agric 83:64–69
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The authors thank the Department of Science and Technology, New Delhi, India. for the financial assistance. (Project no. SR /SO/HS- 21/ 2004)
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Reddy, V.P., Urooj, A. Antioxidant properties and stability of aegle marmelos leaves extracts. J Food Sci Technol 50, 135–140 (2013). https://doi.org/10.1007/s13197-010-0221-z
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DOI: https://doi.org/10.1007/s13197-010-0221-z