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Radioactivity Reduction of 2-Deoxy-2-[18F] Fluoro-D-Glucose by Milk and Ursodeoxycholic Acid in Preclinical Study

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Abstract

Purpose

2-Deoxy-2-[18F] fluoro-d-glucose positron emission tomography (18F-FDG-PET) is a less-invasive and widely used diagnostic tool for detection of malignant tumors. However, prolonged retention of 18F-FDG in the body increases radiation exposure. This study evaluated the effect of oral administration of milk and ursodeoxycholic acid (UDCA) in terms of reducing radiation exposure by 18F-FDG.

Methods

18F-FDG radioactivity was measured using a digital γ counter in the whole body and in various organs of rats after oral administration of milk and milk plus UDCA (milk + UDCA). Western blotting was performed to measure the expression levels of G6Pase, HK 2, CREB, FoxO1, and PGC-1α in the brain, liver, small intestine, and large intestine to assess the mechanism underlying the reduction in radiation exposure from 18F-FDG by oral administration of milk and UDCA.

Results

We found a significant reduction in 18F-FDG radioactivity in the whole body and in the brain, liver, and small and large intestines. Expression of G6Pase was significantly increased in the above-mentioned organs in the milk and milk + UDCA groups. Expression of HK 2 was significantly decreased in the brain and small intestine in the milk and milk + UDCA groups. CREB, FoxO1, and PGC-1α expression levels in the brain, liver, and small intestine were increased in the milk and milk + UDCA groups. However, expression of PGC-1α in the large intestine in the milk and milk + UDCA groups was significantly decreased compared with that in the control group.

Conclusion

The present study demonstrated that administration of milk and UDCA increased G6Pase expression levels and 18F-FDG release from the tissue. These results suggest milk and UDCA could be used to reduce radiation exposure from 18F-FDG after image acquisition. The mechanisms underpinning this phenomenon should be explored in a human study.

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Funding

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI18C0139).

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Author notes

  1. Hwan-Jeong Jeong and Tarique Rajasaheb Bagalkot contributed equally to this work.

Authors and Affiliations

  1. Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Research Institute of Clinical Medicine of Jeonbuk National University and Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, Jeonbuk, Republic of Korea

    Hwan-Jeong Jeong, Hyeon Soo Kim, Yeon-Hee Han, Minjoo Kim, Seok Tae Lim & Myung-Hee Sohn

  2. Department of Cell biology, University of Pittsburgh, Pittsburgh, PA, USA

    Tarique Rajasaheb Bagalkot

Authors
  1. Hwan-Jeong Jeong
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  2. Tarique Rajasaheb Bagalkot
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Correspondence to Myung-Hee Sohn.

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Conflict of Interest

Hwan-Jeong Jeong, Tarique Rajasaheb Bagalkot, Hyeon Soo Kim, Yeon-Hee Han, Minjoo Kim, Seok Tae Lim, and Myung-Hee Sohn declare that they have no conflict of interest.

Ethical Approval

All protocols involving animals were conducted in compliance with the policy and procedure of the Institutional Animal Care and Use Committee of Jeonbuk National University (CBNU 2015-097) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Jeong, HJ., Bagalkot, T.R., Kim, H.S. et al. Radioactivity Reduction of 2-Deoxy-2-[18F] Fluoro-D-Glucose by Milk and Ursodeoxycholic Acid in Preclinical Study. Nucl Med Mol Imaging 54, 105–113 (2020). https://doi.org/10.1007/s13139-020-00634-x

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  • DOI: https://doi.org/10.1007/s13139-020-00634-x

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