Abstract
The ovary is an excellent system for studying stem cell renewal and differentiation, which is under the control of ovarian somatic cells. In order to understand oogenesis in Fugu rubripes (Temminck et. Schlegel) as a marine fish model of aquaculture importance, we established cell lines called TSOC1 and TSOC2 from a juvenile ovary of this organism. TSOC1 is composed of spindle epithelial-like cells, while the other is cobblestone-like cells. Therefore, TSOC1 and TSOC2 appear to consist of ovarian somatic cells. Growth requirement condition was investigated including temperature, concentration of FBS and pH. Significant fluorescent signals were observed after TSOC1 and TSOC2 cells were transfected with pEGFP-N3 vector, indicating its potential utility for genetic manipulation such as gene function studies. It is shown that these cell lines are effective for infection by the turbot reddish body iridovirus and flounder lymphosystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electron microscopy, demonstrating that TSOC1 and TSOC2 are suitable to study interactions between virus and host cells. It is believed that TSOC1 and TSOC2 will be useful tools to study sex-related events and interactions between primordial germ cells and oogonia cells during oogenesis. Therefore, establishment of ovary cell lines from Fugu rubripes seems to be significant for those research areas.
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References
Ahne W. 1985. Studies on the use of fish tissue cultures for toxicity tests in order to reduce and replace the fish tests. Zbl Bakt Hyg, Abt Orig B, 180: 480–504
Bowser P R, Plumb J A. 1980. Fish cell lines: Establishment of a line from ovaries of channel catfish. In Vitro, 16(5): 365–368
Chau R, Kalaitzis J A, Neilan B A. 2011. On the origins and biosynthesis of tetrodotoxin. Aquatic Toxicology, 104(1–2): 61–72
Chen Songlin, Ren Guocheng, Sha Zhenxia, et al. 2005. Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus). Aquaculture, 249(1–4): 63–68
Clark R F, Williams S R, Nordt S P, et al. 1999. A review of selected seafood poisonings. Undersea Hyperb Med, 26(3): 175–184
Frerichs G N, Morgan D, Hart D, et al. 1991. Spontaneously productive C-type retrovirus infection of fish cell lines. J Gener Virol, 72(10): 2537–2539
Fryer J L, Lannan C N. 1994. Three decades of fish cell culture: A current listing of cell lines derived from fishes. Journal of Tissue Culture Methods, 16(2): 87–94
Hightower L H, Renfro J L. 1988. Recent applications of fish cell culture to biomedical research. J Exp Zool, 248(3): 290–302
Hong Yunhan, Liu Tongming, Zhao Haobin, et al. 2004. Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro. Proc Natl Acad Sci USA, 101(21): 8011–8016
Hwang D F, Tai K P, Chueh C H, et al. 1991. Tetrodotoxin and derivatives in several species of the gastropod Naticidae. Toxicon, 29(8): 1019–1024
Komura J-I, Mitani H, Shima A, et al. 1988. Fish cell culture: establishment of two fibroblast-like cell lines (OL-17 and OL-32) from fins of the medaka, Oryzias shima. In Vitro Cell Dev Biol, 24(4): 294–298
Lakra W S, Swaminathan T R, Joy K P. 2011. Development, characterization, conservation and storage of fish cell lines: a review. Fish Physiol Biochem, 37(1): 1–20
Noguchi T, Hwang D F, Arakawa O, et al. 1987. Vibrio alginolyticus, a tetrodotoxin-producing bacterium, in the intestines of the fish Fugu vermicularis vermicularis. Marine Biology, 94(4): 625–630
Sha Zhenxia, Ren Guocheng, Wang Xianli, et al. 2010. Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis). Acta Oceanol Sin, 29(2): 81–87
Sun Xiuqin, Qu Lingyun, Zhang Jinxing, et al. 2000. Pathogenicity and immunogenicity of lymphocystis virus of Japanese flounder (Paralichthys olivaceus). High Technol Lett (in Chinese), 10(9): 19–21
Wang Xianli, Chen Songlin, Sha Zhenxia, et al. 2010a. Establishment and characterization of a new cell line from the kidney of spotted Halibut Verasper variegates. J Ocean Univ China, 9(2): 162–168
Wang N, Wang X L, Sha Z X, et al. 2010b. Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus). Fish Physiol Biochem, 36(4): 1227–1234
Wang X L, Wang N, Sha Z X, et al. 2010c. Establishment, characterization of a new cell line from heart of half-smooth tongue-sole (Cynoglossus semilaevis). Fish Physio Biochem, 36(4): 1181–1189
Watanabe T, Kobayashi N, Sato Y. 1978. Continuous cell line derived from the kidney of yamame, Oncorhynchus masou. Bull Japan Soc Sci Fish, 44(5): 415–418
Wolf K, Quimby M C. 1962. Established eurythermic line of fish cells in vitro. Science, 135(3508): 1065–1066
Zhang Bo, Wang Xiali, Sha Zhenxia, et al. 2011. Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis. International Journal of Biological Sciences, 7(4): 452–459
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Foundation item: The finance special program of Tianjin; the transformation project of Tianjin Agricultural Achievements.
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Zheng, D., Zhang, B., Song, W. et al. Initiation of two ovarian cell lines from Fugu rubripes (Temminck et. Schlegel). Acta Oceanol. Sin. 34, 98–102 (2015). https://doi.org/10.1007/s13131-015-0727-3
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DOI: https://doi.org/10.1007/s13131-015-0727-3