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Glutamate receptors of the A5 region modulate cardiovascular responses evoked from the dorsomedial hypothalamic nucleus and perifornical area

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Abstract

To assess the possible function of glutamate in the interaction between the dorsomedial hypothalamic nucleus-perifornical area (DMH-PeF) and the A5 pontine region (A5), cardiovascular and respiratory changes were studied in response to electrical stimulation of the DMH-PeF (1 ms pulses, 30–50 μA given at 100 Hz for 5 s) before and after the microinjection of kynurenic acid (non-specific glutamate receptor antagonist; 50 nl, 5 nmol), MK-801 (NMDA receptor antagonist; 50 nl, 50 nmol), CNQX (non-NMDA receptor antagonist; 50 nl, 50 nmol) or MCPG (metabotropic glutamate receptor antagonist; 50 nl, 5 nmol) within the A5 region. DMH-PeF electrical stimulation elicited a pressor (p < 0.001) and tachycardic response (p < 0.001) which was accompanied by an inspiratory facilitation characterised by an increase in respiratory rate (p < 0.001) due to a decrease in expiratory time (p < 0.01). Kynurenic acid within the A5 region decreased the tachycardia (p < 0.001) and the intensity of the blood pressure response (p < 0.001) to DMH-PeF stimulation. After the microinjection of MK-801 and CNQX into the A5 region, the magnitude of the tachycardia and the pressor response were decreased (p < 0.05 and p < 0.01; p < 0.001 and p < 0.05, respectively). After MCPG microinjection into the A5 region, a decrease in the tachycardia (p < 0.001) with no changes in the pressor response was observed during DMH-PeF stimulation. The respiratory response elicited by DMH-PeF stimulation was not changed after the microinjection of kynurenic acid, MK-801, CNQX or MCPG within the A5 region. These results suggest that A5 region glutamate receptors play a role in the cardiovascular response elicited from the DMH-PeF. The possible mechanisms involved in these interactions are discussed.

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Funding

The study was supported by a program grant Junta de Andalucía, Grupo Consolidado no. CTS156, Spain. Part of the final study was supported with a grant from the Own Funds Program of the University of Málaga.

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Authors and Affiliations

  1. Departamento de Fisiología Humana, Histología Humana, Anatomía Patológica y Educación Física y Deportiva, Facultad de Medicina, Universidad de Málaga, Málaga, Spain

    M. V. López-González, A. Díaz-Casares, M. González-García, C. A. Peinado-Aragonés, M. A. Barbancho, M. Carrillo de Albornoz & M. S. Dawid-Milner

  2. Unidad de Neurofisiología del Sistema Nervioso Autónomo (CIMES), Universidad de Málaga, Málaga, Spain

    M. V. López-González, A. Díaz-Casares, M. González-García & M. S. Dawid-Milner

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  1. M. V. López-González
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  2. A. Díaz-Casares
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  3. M. González-García
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  4. C. A. Peinado-Aragonés
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  5. M. A. Barbancho
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  6. M. Carrillo de Albornoz
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  7. M. S. Dawid-Milner
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Correspondence to M. V. López-González.

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López-González, M.V., Díaz-Casares, A., González-García, M. et al. Glutamate receptors of the A5 region modulate cardiovascular responses evoked from the dorsomedial hypothalamic nucleus and perifornical area. J Physiol Biochem 74, 325–334 (2018). https://doi.org/10.1007/s13105-018-0623-3

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  • DOI: https://doi.org/10.1007/s13105-018-0623-3

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