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Dietary cladode powder from wild type and domesticated Opuntia species reduces atherogenesis in apoE knock-out mice

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Abstract

Dietary intake of Opuntia species may prevent the development of cardiovascular diseases. The present study was designed to characterize the biological antioxidant and anti-inflammatory properties of Opuntia species and to investigate whether Opuntia cladodes prevent the development of atherosclerosis in vivo, in apoE−KO mice. The effects of the two Opuntia species, the wild Opuntia streptacantha and the domesticated Opuntia ficus-indica, were tested on the generation of intra- and extracellular reactive oxygen species (ROS) production and kinetics of the LDL oxidation by murine CRL2181 endothelial cells and on the subsequent inflammatory signaling leading to the adhesion of monocytes on the activated endothelium and the formation of foam cells. Opuntia species blocked the extracellular ROS (superoxide anion) generation and LDL oxidation by CRL2181, as well as the intracellular ROS rise and signaling evoked by the oxidized LDL, including the nuclear translocation of the transcription factor NFκB, the expression of ICAM-1 and VCAM-1 adhesion molecules, and the adhesion of monocytes to CRL2181. In vivo, Opuntia significantly reduced the formation of atherosclerotic lesions and the accumulation of 4-hydroxynonenal adducts in the vascular wall of apoE-KO mice, indicating that Opuntia cladodes prevent lipid oxidation in the vascular wall. In conclusion, wild and domesticated Opuntia species exhibit antioxidant, anti-inflammatory, and antiatherogenic properties which emphasize their nutritional benefit for preventing cardiovascular diseases.

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Acknowledgements

This work was supported by INSERM, University Toulouse 3, and by the bilateral French/Mexican ANR (French National Research Agency)/CONACYT (National council of Science and Technology of Mexico) project BIOPUNTIA (ANR-2010-INTB-1702). The excellent technical assistance of Thomas Farge, Marie Hélène Grazide, and Corinne Bernis is gratefully acknowledged.

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Authors and Affiliations

  1. INSERM UMR 1048, BP84225 - 31432, Toulouse, Cedex 4, France

    Sandra Garoby-Salom, Caroline Camaré, Robert Salvayre & Anne Negre-Salvayre

  2. University of Toulouse, Toulouse, France

    Sandra Garoby-Salom, Caroline Camaré, Robert Salvayre & Anne Negre-Salvayre

  3. UMR1331 INRA/UPS/INPT Toxalim, Toulouse, France

    Françoise Guéraud

  4. IPICyT, Instituto Potosino de Investigación Científica y Tecnológica, A.C, San Luis Potosi, Mexico

    Ana-Paulina Barba de la Rosa

  5. IPBS, Toulouse, France

    Michel Rossignol

  6. Faculty of Chemistry, UASLP, San Luis Potosí, Mexico

    María del Socorro Santos Díaz

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  1. Sandra Garoby-Salom
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  2. Françoise Guéraud
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  3. Caroline Camaré
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  4. Ana-Paulina Barba de la Rosa
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Correspondence to Anne Negre-Salvayre.

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Garoby-Salom, S., Guéraud, F., Camaré, C. et al. Dietary cladode powder from wild type and domesticated Opuntia species reduces atherogenesis in apoE knock-out mice. J Physiol Biochem 72, 59–70 (2016). https://doi.org/10.1007/s13105-015-0461-5

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  • DOI: https://doi.org/10.1007/s13105-015-0461-5

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