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S-resistin, a non secretable resistin isoform, impairs the insulin signalling pathway in 3T3-L1 adipocytes

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Abstract

S-resistin is a non-secretable resistin spliced variant, which is expressed mainly in the white adipose tissue from Wistar rats. Previous results confirmed that 3T3-L1 cells expressing s-resistin (3T3-L1-s-res) showed an inflammatory response and exhibited a decrease in glucose transport, both basal and insulin-stimulated. Here we present evidences demonstrating for the first time that s-resistin, like resistin, blocks insulin signalling pathway by inhibiting insulin receptor, insulin receptor substrate 1, protein kinase B/Akt and the mammalian target of rapamycin phosphorylation, and increasing the suppressor of cytokine signalling 3 levels being the later probably due to augmented of leptin expression. Thus, our data suggest that s-resistin could act by a still unknown intracrine pathway as an intracellular sensor, regulating the adipocyte insulin sensitivity.

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Acknowledgments

MR was a recipient of a predoctoral fellowship from Universidad de Castilla-La Mancha and from BFU2012-39705-C03-01 from Ministerio de Ciencia e Innovación (Spain). This work was supported by research grants PI-2007/60 from Junta de Comunidades de Castilla-La Mancha (JCCM) FISCAM; DGI-BFU2008-C03-02/BFI and BFU2012-39705-C03-01 from Ministerio de Ciencia e Innovación (Spain). The helpful comments and suggestions of PhD. A. del Arco are acknowledged.

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Authors and Affiliations

  1. Área de Bioquímica. Facultad de Ciencias Ambientales y Bioquímica, Centro Regional de Investigaciones Biomédicas (CRIB), Universidad de Castilla-La Mancha, Av. Carlos III s/n, 45071, Toledo, Spain

    María Rodríguez, Eduardo Moltó, Lidia Aguado & Carmen Arribas

  2. Área de Bioquímica. Facultad de Ciencias y Tecnologías Químicas, Centro Regional de Investigaciones Biomédicas (CRIB), Universidad de Castilla-La Mancha, Av Camilo José Cela s/n, 13071, Ciudad Real, Spain

    Nilda Gallardo & Antonio Andrés

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  1. María Rodríguez
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  2. Eduardo Moltó
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  3. Lidia Aguado
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  6. Carmen Arribas
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Correspondence to Carmen Arribas.

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Rodríguez, M., Moltó, E., Aguado, L. et al. S-resistin, a non secretable resistin isoform, impairs the insulin signalling pathway in 3T3-L1 adipocytes. J Physiol Biochem 71, 381–390 (2015). https://doi.org/10.1007/s13105-015-0418-8

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  • DOI: https://doi.org/10.1007/s13105-015-0418-8

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