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Role of angiotensin II and oxidative stress on renal aquaporins expression in hypernatremic rats

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Abstract

The aim of this study was to assess whether endogenous Ang II and oxidative stress produced by acute hypertonic sodium overload may regulate the expression of aquaporin-1 (AQP-1) and aquaporin-2 (AQP-2) in the kidney. Groups of anesthetized male Sprague–Dawley rats were infused with isotonic saline solution (control) or with hypertonic saline solution (Na group, 1 M NaCl), either alone or with losartan (10 mg kg−1) or tempol (0.5 mg min−1 kg−1) during 2 h. Renal function parameters were measured. Groups of unanesthetized animals were injected intraperitoneally with hypertonic saline solution, with or without free access to water intake, Na+W, and Na−W, respectively. The expression of AQP-1, AQP-2, Ang II, eNOS, and NF-kB were evaluated in the kidney by Western blot and immunohistochemistry. AQP-2 distribution was assessed by immunofluorescence. Na group showed increased natriuresis and diuresis, and Ang II and NF-kB expression, but decreased eNOS expression. Losartan or tempol enhanced further the diuresis, and AQP-2 and eNOS expression, as well as decreased Ang II and NF-kB expression. Confocal immunofluorescence imaging revealed labeling of AQP-2 in the apical plasma membrane with less labeling in the intracellular vesicles than the apical membrane in kidney medullary collecting duct principal cells both in C and Na groups. Importantly, our data also show that losartan and tempol induces a predominantly accumulation of AQP-2 in intracellular vesicles. In unanesthetized rats, Na+W group presented increased diuresis, natriuresis, and AQP-2 expression (112 ± 25 vs 64 ± 16; *p < 0.05). Water deprivation increased plasma sodium and diuresis but decreased AQP-2 (46 ± 22 vs 112 ± 25; §p < 0.05) and eNOS expression in the kidney. This study is a novel demonstration that renal endogenous Ang II–oxidative stress, induced in vivo in hypernatremic rats by an acute sodium overload, regulates AQP-2 expression.

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Acknowledgments

This study was supported by grants from the National Scientific and Technical Research Council (CONICET, PIP 1337) and Universidad de Buenos Aires, Argentina (UBACYT B113, 20020100100688, and 20020110200048)

Conflict of interests

All authors declare no conflict of interests.

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Authors and Affiliations

  1. Department of Pathophysiology, University of Buenos Aires, INFIBIOC-CONICET, Buenos Aires, Argentina

    Silvana L. Della Penna, Nicolás M. Kouyoumdzian, Lorena Sarati, Andrea Fellet, Ana M. Balaszczuk, Marcelo R. Choi, Elsa Zotta, María I. Rosón & Belisario E. Fernández

  2. Department of Pharmacology, University of Buenos Aires, INFIBIOC-CONICET, Buenos Aires, Argentina

    Susana Gorzalczany

  3. Department of Clinical Biochemistry, School of Pharmacy and Biochemistry, University of Buenos Aires, INFIBIOC-CONICET, Buenos Aires, Argentina

    Marcela Pandolfo

  4. Laboratory of Experimental Medicine, Hospital Alemán, Buenos Aires, Argentina

    Gabriel Cao & Jorge E. Toblli

  5. Cátedra de Fisiopatología, Facultad de Farmacia y Bioquímica, Junín 956, Buenos Aires, 1113, Argentina

    Silvana L. Della Penna

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  1. Silvana L. Della Penna
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  2. Gabriel Cao
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  3. Nicolás M. Kouyoumdzian
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  4. Lorena Sarati
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  6. Ana M. Balaszczuk
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  7. Marcelo R. Choi
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  8. Elsa Zotta
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  9. Susana Gorzalczany
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  10. Marcela Pandolfo
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  11. Jorge E. Toblli
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  12. María I. Rosón
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  13. Belisario E. Fernández
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Correspondence to Silvana L. Della Penna.

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Della Penna, S.L., Cao, G., Kouyoumdzian, N.M. et al. Role of angiotensin II and oxidative stress on renal aquaporins expression in hypernatremic rats. J Physiol Biochem 70, 465–478 (2014). https://doi.org/10.1007/s13105-014-0324-5

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  • DOI: https://doi.org/10.1007/s13105-014-0324-5

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