Skip to main content

Advertisement

SpringerLink
Log in

Changes of activity and kinetics of certain liver and heart enzymes of hypothyroid and T3-treated rats

  • Original Paper
  • Published:
Journal of Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

Thyroid diseases are one of the most common metabolic disorders in the human population. In this work, we present data concerning changes in the activity and kinetic parameters of several enzymes associated with both anabolic (glucose-6-phosphate dehydrogenase—G6PDH, EC 1.1.1.49; 6-phosphogluconate dehydrogenase—6PGDH, EC 1.1.1.44; malic enzyme—ME, EC 1.1.1.40; and isocitrate dehydrogenase—IDH, EC 1.1.1.42) and catabolic (NAD-dependent malate dehydrogenase—NAD-MDH, EC 1.1.1.37; and lactate dehydrogenase—LDH, EC 1.1.1.27) processes under conditions of hypothyroidism and T3 treatment. Hypothyroidism was induced in rats by the surgical removal of the thyroid gland. T3-treated rats were injected by T3 (0.5 mg T3/kg body weight daily during 10 days). We have found that T3 treatment caused an increase of NAD-MDH activity as well as heart hypertrophy whereas the activity of LDH increased in the direction of pyruvate reduction. Moreover, we observed increased activity of both enzymes in the liver. These results confirm earlier observation concerning the relevance of oxidative metabolism in the heart under T3 treatment. Hypothyroidism resulted in changes in the LDH activity in the heart whereas NAD-MDH activity did not change. Moreover, our data show that T3 treatment caused an increase of G6PDH, 6PGDH, and ME activities in the liver. We also observed a decrease of IDH activity in both organs, whereas hypothyroidism caused the opposite effect. This data indicate that either deficiency or excess of thyroid hormones can prove to be particularly dangerous for the physiology of the heart muscle by disturbing bioenergetic and anabolic processes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Baxter JD, Dillmann WH, West BL, Huber R, Furlow JD, Fletterick RJ, Webb P, Apriletti JW, Scanlan TS (2001) Selective modulation of thyroid hormone receptor action. J Steroid Biochem Mol Biol 76:31–42

    Article  PubMed  CAS  Google Scholar 

  2. Cooper DS (2003) Hyperthyroidism. Lancet 362:459–468

    Article  PubMed  CAS  Google Scholar 

  3. Grant N (2007) The role of triiodothyronine-induced substrate cycles in the hepatic response to overnutrition: thyroid hormone as an antioxidant. Med Hypotheses 68:641–649

    Article  PubMed  CAS  Google Scholar 

  4. Horn S, Heuer H (2010) Thyroid hormone action during brain development: more questions than answers. Mol Cell Endocrinol 315:19–26

    Article  PubMed  CAS  Google Scholar 

  5. Kahaly GJ, Dillmann WH (2005) Thyroid hormone action in the heart. Endocr Rev 26:704–728

    Article  PubMed  CAS  Google Scholar 

  6. Kuncova J, Slawikowa J (2000) Vasoactive intestinal polypeptide in rat heart atria: the effect of hyperthyroidism. Physiol Res 49:427–434

    PubMed  CAS  Google Scholar 

  7. Lewandowski KC, Rechcinski T, Krzeminska-Pakula M, Lewinski A (2010) Acute myocardial infarction as the first presentation of thyrotoxicosis in a 31-year old woman—case report. Thyroid Res 3:1

    Article  PubMed  Google Scholar 

  8. Lombardi A, Beneduce L, Moreno M, Diano S, Colantuoni V, Ursini MV, Lanni A, Goglia F (2000) 3,5-diiodo-L-thyronine regulates glucose-6-phosphate dehydrogenase activity in the rat. Endocrinology 141:1729–1734

    Article  PubMed  CAS  Google Scholar 

  9. Lowry PH, Rosebrough HJ, Farr AL, Randall R (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  10. Maglysh SS, Gorbach ZV, Ostrovski YM (1982) Purification and some properties of molecular forms of 6-phosphogluconate dehydrogenase from rat liver. Biokhimiia 47:2035–2041

    PubMed  CAS  Google Scholar 

  11. Messarah M, Boumendjel A, Chouabia A, Klibet F, Abdennour C, Boulakoud MS, El Feki A (2010) Influence of thyroid dysfunction on liver lipid peroxidation and antioxidant status in experimental rats. Exp Toxicol Pathol 62:301–310

    Article  PubMed  CAS  Google Scholar 

  12. Miksicek RJ, Towle HC (1983) Use of a cloned cDNA sequence to measure changes in 6-phosphogluconate dehydrogenase mRNA levels caused by thyroid hormone and dietary carbohydrate. J Biol Chem 258:9575–9579

    PubMed  CAS  Google Scholar 

  13. Moro L, Marra E, Capuano F, Greco M (2004) Thyroid hormone treatment of hypothyroid rats restores the regenerative capacity and the mitochondrial membrane permeability properties of the liver after partial hepatectomy. Endocrinology 145:5121–5128

    Article  PubMed  CAS  Google Scholar 

  14. Nygaard B (2007) Hyperthyroidism. Am Fam Physician 76:1014–1016

    PubMed  Google Scholar 

  15. Oetting A, Yen PM (2007) New insights into thyroid hormone action. Best Pract Res Clin Endocrinol Metab 21:193–208

    Article  PubMed  CAS  Google Scholar 

  16. Poppe K, Glinoer D (2003) Thyroid autoimmunity and hypothyroidism before and during pregnancy. Hum Reprod Update 9:149–161

    Article  PubMed  Google Scholar 

  17. Ralphe JC, Bedell K, Segar JL, Scholz TD (2005) Correlation between myocardial malate/aspartate shuttle activity and EAAT1 protein expression in hyper- and hypothyroidism. Am J Physiol Heart Circ Physiol 288:2521–2526

    Article  Google Scholar 

  18. Rosenkrans CF Jr, Coffey KP, Paria BC, Tarn CY, Johnson ZB, Moyer JL (2000) Effects of fescue and clover forage on serum lactate dehydrogenase and glucose 6-phosphate dehydrogenase isoenzymic profiles in steers. Vet Hum Toxicol 42:337–340

    PubMed  Google Scholar 

  19. Song Y, Yao X, Ying H (2011) Thyroid hormone action in metabolic regulation. Protein Cell 2:358–368

    Article  PubMed  CAS  Google Scholar 

  20. Tylicki A, Czerniecki J, Godlewska A, Kieliszek M, Zebrowski T, Bielawski T, Wojcik B (2008) Changes in ECG and enzyme activity in rat hart after myocardial infarction: effect of TPP and MnCl2. J Physiol Biochem 64:93–102

    Article  PubMed  CAS  Google Scholar 

  21. Tylicki A, Kawalko A, Sokolska J, Strumilo S (2007) Effect of anabolic steroid nandrolone decanoate on the properties of certain enzymes in the heart, liver and muscle of rats, and their effect on rats’ cardiac electrophysiology. Horm Metab Res 39:268–272

    Article  PubMed  CAS  Google Scholar 

  22. Weitzel JM, Iwen KAH, Seitz HJ (2003) Regulation of mitochondrial biogenesis by thyroid hormone. Exp Physiol 88(1):121–128

    Article  PubMed  CAS  Google Scholar 

  23. Wu Y, Koenig RJ (2000) Gene regulation by thyroid hormone. Trends Endocrinol Metab 11:207–211

    Article  PubMed  CAS  Google Scholar 

  24. Yen PM (2001) Physiological and molecular basis of thyroid hormone action. Physiol Rev 81:1097–1114

    PubMed  CAS  Google Scholar 

  25. Yun-Bo S, Ritchie JWA, Taylor PM (2002) Complex regulation of thyroid hormone action: multiple opportunities for pharmacological intervention. Pharmacol Ther 94:235–251

    Article  Google Scholar 

  26. Zulewski H (2011) Hypothyroidism. Ther Umsch 68:315–320

    PubMed  Google Scholar 

Download references

Acknowledgments

We thank Jan Czerniecki (Department of Cytobiochemistry, University of Bialystok) and our students Marta Drobinska, Aneta Wlodkowska, Barbara Wlodkowska, and Renata Zaniewska for their excellent technical assistance.

Author information

Authors and Affiliations

  1. Department of Cytobiochemistry, Institute of Biology, University of Bialystok, ul. Swierkowa 20B, 15-950, Bialystok, Poland

    Urszula Czyzewska, Adam Tylicki, Magdalena Siemieniuk & Slawomir Strumilo

Authors
  1. Urszula Czyzewska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Adam Tylicki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Magdalena Siemieniuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Slawomir Strumilo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adam Tylicki.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Czyzewska, U., Tylicki, A., Siemieniuk, M. et al. Changes of activity and kinetics of certain liver and heart enzymes of hypothyroid and T3-treated rats. J Physiol Biochem 68, 345–351 (2012). https://doi.org/10.1007/s13105-012-0146-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13105-012-0146-2

Keywords

Search

Navigation