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Diversity assessment of drought tolerant exotic and adapted maize (Zea mays L.) inbred lines with microsatellite markers

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Abstract

Knowledge about the genetic diversity within a germplasm allows for a more effective and efficient use of resources for crop improvement programs. Diversity assessment of 48 tropical drought-tolerant maize inbreds, 24 each from CIMMYT and IITA, was carried out with microsatellite markers to determine the genetic divergence between the two groups of maize inbred lines. Eighty-one polymorphic SSR markers were used for the assessment. Results showed that the average number of alleles per locus was 3.7. The mean polymorphic information content (PIC) was 0.51 whereas the average modified Roger’s genetic distance (MRD) was 0.49. The mean genetic distance estimates between the CIMMYT and IITA lines were higher than the mean genetic distance among IITA lines or among CIMMYT lines. The average linkage cluster analysis separated the lines into two broad groups along institutional lines. The observed sub-groups within each main group were reflections of the relationships of the lines based on pedigree records. The F ST value of 19.5% reflects a high level of genetic differentiation between the two groups of lines. The results highlighted the presence of appreciable genetic divergence between CIMMYT and IITA lines that can be exploited for superior hybrid maize development.

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Authors and Affiliations

  1. West Africa Centre for Crop Improvement, P. O. Box 30, Legon, Ghana

    Moses Adeolu Adebayo, Essie Blay, Vernon Gracen & Eric Danquah

  2. International Institute of Tropical Agriculture, PMB 5320, Ibadan, Nigeria

    Moses Adeolu Adebayo, Abebe Menkir, Malaku Gedil & Ladejobi Funmilayo

  3. Department of Crop Production and Soil Science, Ladoke Akintola University of Technology, PMB 4000, Ogbomoso, Nigeria

    Moses Adeolu Adebayo

Authors
  1. Moses Adeolu Adebayo
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  2. Abebe Menkir
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  3. Malaku Gedil
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  4. Essie Blay
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  5. Vernon Gracen
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  6. Eric Danquah
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  7. Ladejobi Funmilayo
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Correspondence to Abebe Menkir.

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Adebayo, M.A., Menkir, A., Gedil, M. et al. Diversity assessment of drought tolerant exotic and adapted maize (Zea mays L.) inbred lines with microsatellite markers. J. Crop Sci. Biotechnol. 18, 147–154 (2015). https://doi.org/10.1007/s12892-014-0076-3

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  • DOI: https://doi.org/10.1007/s12892-014-0076-3

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