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Characterization of polymorphic microsatellite markers for the tideland snail Batillaria flectosiphonata

  • Microsatellite Letters
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Abstract

Batillaria flectosiphonata is distributed in the Ryukyu Islands, south-western Japan, and shows genetic differentiation among island groups. This species is vulnerable to environmental fluctuations because the connectivity among the groups is low. Therefore, the conservation of each group is necessary to maintain their genetic diversity. Using next-generation sequencing, 10 microsatellite markers were developed for B. flectosiphonata. In Miyara Bay and the Sakashita River estuary, the numbers of alleles per locus were 1–9 and 1–8, and the expected heterozygosities were 0–0.859 and 0–0.655, respectively.

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Acknowledgments

This study was supported by the Interdisciplinary Collaborative Research Program of the Atmosphere and Ocean Research Institute at the University of Tokyo and a Sasakawa Scientific Research Grant from the Japan Science Society. We are also grateful to Junya Hirai at the National Research Institute of Fisheries Science for supporting our research.

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Authors and Affiliations

  1. Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba, 277-8564, Japan

    Hajime Itoh, Kimiko Hirose & Shigeaki Kojima

  2. Graduate School of Urban Engineering, Osaka City University, 3-3-138, Sugimoto, Sumiyoshi, Osaka, 558-8585, Japan

    Satomi Kamimura

Authors
  1. Hajime Itoh
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  2. Satomi Kamimura
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  3. Kimiko Hirose
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  4. Shigeaki Kojima
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Corresponding author

Correspondence to Hajime Itoh.

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Itoh, H., Kamimura, S., Hirose, K. et al. Characterization of polymorphic microsatellite markers for the tideland snail Batillaria flectosiphonata . Conservation Genet Resour 7, 751–753 (2015). https://doi.org/10.1007/s12686-015-0452-4

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  • DOI: https://doi.org/10.1007/s12686-015-0452-4

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