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Microsatellite markers for a hyperparasitoid wasp from a fragmented landscape

  • Microsatellite Letters
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Abstract

We developed 31 microsatellite loci for a specialist hyperparasitoid wasp, Mesochorus cf. stigmaticus that parasitizes the parasitoid Hyposoter horticola, in Åland islands of southwestern Finland. The microsatellites were developed from 454 sequencing of the enriched libraries. The details of primers and the polymerase chain reactions for these microsatellite markers are described. All the loci tested on 24 samples were polymorphic with on average 4.1 alleles per locus (range 2–13). Genetic diversities as measured by average expected heterozygosity (HE) and allelic richness (AR) were generally high (HE ≈ 0.10–0.80; AR ≈ 1.9–11.5). These markers will be useful in population genetic studies of this parasitoid and other related taxa.

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Acknowledgments

We thank S. Bogdanowicz for construction of microsatellite library and Academy of Finland for funding the project (Grant Nos. 250444, 213547, 125553 to S. van Nouhuys).

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Authors and Affiliations

  1. Metapopulation Research Group, Department of Biosciences, University of Helsinki, P.O. Box 65, 00014, Helsinki, Finland

    Abhilash Nair & Saskya van Nouhuys

  2. Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY, 14853, USA

    Saskya van Nouhuys

Authors
  1. Abhilash Nair
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  2. Saskya van Nouhuys
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Correspondence to Abhilash Nair.

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Nair, A., van Nouhuys, S. Microsatellite markers for a hyperparasitoid wasp from a fragmented landscape. Conservation Genet Resour 7, 565–568 (2015). https://doi.org/10.1007/s12686-015-0425-7

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  • DOI: https://doi.org/10.1007/s12686-015-0425-7

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