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Isolation and characterisation of ten microsatellite loci from a Western Australian tree, Banksia sessilis (Proteaceae)

  • Microsatellite Letters
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Abstract

Ten nuclear microsatellite markers were developed for Banksia sessilis, a tree endemic to Western Australia to assess whether patterns of genetic diversity and structure reflect the taxonomic varieties currently described, one of which is a priority species for conservation. One tetranucleotide, three trinucleotide and six dinucleotide repeat loci were tested on 24 individuals from each of two populations. All loci showed independent inheritance and were polymorphic. The number of alleles ranged from two to seven. Observed heterozygosity ranged from 0.083 to 0.750 and expected heterozygosity from 0.080 to 0.718.

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Acknowledgments

We thank Bronwyn MacDonald for help with DNA extractions.

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Authors and Affiliations

  1. Science and Conservation Division, Department of Parks and Wildlife, Bentley Delivery Centre, Locked Bag 104, Bentley, WA, 6983, Australia

    Heidi Nistelberger, Shelley McArthur, David Coates & Margaret Byrne

Authors
  1. Heidi Nistelberger
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  2. Shelley McArthur
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  3. David Coates
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  4. Margaret Byrne
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Corresponding author

Correspondence to Heidi Nistelberger.

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Nistelberger, H., McArthur, S., Coates, D. et al. Isolation and characterisation of ten microsatellite loci from a Western Australian tree, Banksia sessilis (Proteaceae). Conservation Genet Resour 7, 513–515 (2015). https://doi.org/10.1007/s12686-014-0409-z

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  • DOI: https://doi.org/10.1007/s12686-014-0409-z

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