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Characterization of sixteen polymorphic microsatellite DNA loci in the chum salmon (Oncorhynchus keta) isolated by next-generation sequencing

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Abstract

Chum salmon (Oncorhynchus keta) is an important fisheries resource around the North Pacific region. However, the genetic characteristics of this species mostly remain to be elucidated with competent molecular genetic markers for their effective resource management and sustainable use. A total of sixteen novel microsatellite markers in chum salmon were isolated using a next-generation sequencing approach. All loci were polymorphic with 6–21 alleles, with the observed and expected heterozygosities of 0.41667–0.97917 and 0.66184–0.92434, respectively. These new loci will provide a tool for examining the genetic diversity and population structure of chum salmon, which are an important information for the adaptive management of this species.

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Acknowledgments

We thanks to Fisheries Cooperative Association of Shimoakka, Noda, Iwate, for their help in collecting samples. This study was supported by MEXT Revitalization Project for the Creation of Fisheries Research and Education Center in Sanriku, and JST Revitalization Promotion Program.

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Authors and Affiliations

  1. Sanriku Fisheries Research Center, Iwate University, Kamaishishi Heita 3-75-1, kamaishi, 026-0001, Japan

    H. Tsukagoshi, S. Terui & S. Abe

Authors
  1. H. Tsukagoshi
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  2. S. Terui
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  3. S. Abe
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Correspondence to H. Tsukagoshi.

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Tsukagoshi, H., Terui, S. & Abe, S. Characterization of sixteen polymorphic microsatellite DNA loci in the chum salmon (Oncorhynchus keta) isolated by next-generation sequencing. Conservation Genet Resour 7, 173–175 (2015). https://doi.org/10.1007/s12686-014-0322-5

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  • DOI: https://doi.org/10.1007/s12686-014-0322-5

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