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Isolation and characterization of 26 novel polynucleotide microsatellites from short barbeled grunter (Hapalogenys nitens) for genetic analysis

  • Microsatellite Letters
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Abstract

Short barbeled grunter, Hapalogenys nitens, is an important fishery resource and it has been regarded as a target marine fish species for prospective aquaculture diversification in Korea, but sustainable fry production is not yet possible. This delay has led to an increase in the need for genetic information about this species. Furthermore, it is necessary to conserve the wild stocks for the sustainable use of the species. In this study, we report 26 novel polymorphic microsatellites with a mean of 4.9 alleles per locus. The mean observed and expected heterozygosity per locus was 0.45 and 0.50, respectively. Cross-amplification revealed variability in others two Haemulidae species. These markers developed can be a valuable tool for conservation genetics and further genetic improvement of these species.

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Acknowledgments

This work is funded by a grant from the National Fisheries Research and Development (NFRDI) and is contribution number, RP-14-BT-000. Views expressed herein are those of the authors and do not necessarily reflect the views of NFRDI.

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Authors and Affiliations

  1. Biotechnology Research Division, National Fisheries Research and Development Institute, Pusan, 619-705, Korea

    Hye Suck An, Jung Youn Park & Chul Min An

  2. Aquaculture Management Division, Southwest Sea Fisheries Research Institute, National Fisheries Research and Development Institute, Yeosu, 556-823, Korea

    Hee Woong Kang, Hyon Sob Han & Jeong In Myeong

Authors
  1. Hye Suck An
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  2. Hee Woong Kang
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  3. Hyon Sob Han
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  4. Jung Youn Park
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  5. Jeong In Myeong
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  6. Chul Min An
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Corresponding author

Correspondence to Hye Suck An.

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An, H.S., Kang, H.W., Han, H.S. et al. Isolation and characterization of 26 novel polynucleotide microsatellites from short barbeled grunter (Hapalogenys nitens) for genetic analysis. Conservation Genet Resour 6, 669–672 (2014). https://doi.org/10.1007/s12686-014-0177-9

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  • DOI: https://doi.org/10.1007/s12686-014-0177-9

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