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Characterization of 22 polymorphic microsatellite markers for seven-band grouper Epinephelus septemfasciatus developed using a 454 pyrosequencing approach

  • Microsatellite Letters
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Abstract

Seven-band grouper, Epinephelus septemfasciatus, is of considerable economic value and a candidate species of aquaculture in Eastern Asia. It is necessary to monitor the stocks for the sustainable use and aquaculture of the species. Monitoring genetic variation among marine resources necessitates the development of genetic markers. Here, we report 22 polymorphic microsatellite markers with 2–16 alleles per locus. The mean observed and expected heterozygosity per locus was 0.53 and 0.57, respectively. Cross-amplification revealed variability in Epinephelus bruneus and Epinephelus akaara. The 22 markers developed can be a valuable tool for genetic conservation of this species.

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Acknowledgments

This work is funded by a grant from the National Fisheries Research and Development (NFRDI) and is contribution number, RP-14-BT-000. Views expressed herein are those of the authors and do not necessarily reflect the views of NFRDI.

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Authors and Affiliations

  1. Biotechnology Research Division, National Fisheries Research and Development Institute, Pusan, 619-705, Korea

    Hye Suck An & Chul Min An

  2. Aquaculture Management Division, Southwest Sea Fisheries Research Institute, National Fisheries Research and Development Institute, Yeosu, 556-823, Korea

    Jae Kwon Cho, Kyong Min Kim, Maeng Hyun Son & Jeong In Myeong

Authors
  1. Hye Suck An
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  2. Jae Kwon Cho
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  3. Kyong Min Kim
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  4. Maeng Hyun Son
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  5. Jeong In Myeong
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  6. Chul Min An
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Corresponding author

Correspondence to Hye Suck An.

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An, H.S., Cho, J.K., Kim, K.M. et al. Characterization of 22 polymorphic microsatellite markers for seven-band grouper Epinephelus septemfasciatus developed using a 454 pyrosequencing approach. Conservation Genet Resour 6, 665–667 (2014). https://doi.org/10.1007/s12686-014-0176-x

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  • DOI: https://doi.org/10.1007/s12686-014-0176-x

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