Skip to main content
SpringerLink
Log in

Isolation and characterization of 16 polymorphic microsatellite loci in the leopard coral grouper Plectropomus leopardus

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Plectropomus leopardus is an important commercial fish in South-East Asia. To date, there is little genetic information available about its population structure. In this study, 16 polymorphic microsatellite loci were developed for this species and characterized in 182 wild individuals. The observed and expected heterozygosity ranged from 0.236 to 0.912 and from 0.649 to 0.866, respectively. No linkage disequilibrium was detected between loci, and four loci were found to significantly depart from Hardy–Weinberg equilibrium. These polymorphic microsatellite markers are valuable for relative studies of population genetics.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Fischer D, Bachmann K (1989) Microsatellite enrichment in organisms with large genomes (Allium cepa L.). Biotechniques 24:796–802

    Google Scholar 

  • Frisch AJ, Anderson TA (2000) The response of coral trout (Plectropomus leopardus) to capture, handling and transport and shallow water stress. Fish Physiol Biochem 23:23–34

    Article  CAS  Google Scholar 

  • Frisch AJ, McCormick MI, Pankhurst NW (2007) Reproductive periodicity and steroid hormone profiles in the sex-changing coral-reef fish, Plectropomus leopardus. Coral Reefs 26:189–197

    Article  Google Scholar 

  • Herwerden LV, Choat JH, Dudgeon CL, Carlos G, Newman SJ, Frisch A, Oppen MV (2006) Contrasting patterns of genetic structure in two species of the coral trout Plectropomus (Serranidae) from east and west Australia: introgressive hybridization or ancestral polymorphisms. Mol Phylogenet Evol 41:420–435

    Article  PubMed  Google Scholar 

  • Light PR, Jones GP (1997) Habitat preference in newly settled coral trout (Plectropomus leopardus Serranidae). Coral Reefs 16(2):117–126

    Article  Google Scholar 

  • Pritchard VL, Metcalf JL, Jones K, Martin AP, Cowley DE (2009) Population structure and genetic management of Rio Grande cutthroat trout (Oncorhynchus clarkii virginalis). Conserv Genet 10:1209–1221

    Article  Google Scholar 

  • Qu M, Ding SX, Xu XJ, Shen MH, You YZ, Su YQ (2012) Ontogenetic development of the digestive system and growth in coral trout (Plectropomus leopardus). Aquaculture 334–337:132–141

    Article  Google Scholar 

  • Rice WE (1989) Analyzing tables of statistical tests. Evolution 43:223–225

    Article  Google Scholar 

  • Sadovy Y, Donaldson TJ, Graham TR, McGilvray F, Muldoon GJ, Phillips MJ, Rimmer MA, Smith A, Yeeting B (2003) While stocks last: the live reef food fish trade. Pacific Studies Series. Asian Development Bank, Manila 146 p

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

    Google Scholar 

  • Schneider S, Roessli D, Excoffier L (2000) ARLEQUIN: a software for population genetics date analysis, version 2.000. Genetics and Biometry Laboratory, Department of Anthropology, University of Geneva, Geneva, Switzerland

  • Williamson DH, Russ GR, Ayling AM (2004) No-take marine reserves increase abundance and biomass of reef fish on inshore fringing reefs of the Great Barrier Reef. Environ Conserv 31:149–159

    Article  Google Scholar 

  • Yeh FC, Yang R, Boyle, TJ, Ye Z, Xiyan JM (2000) POPGENE 32, Microsoft window-based freeware for population genetic analysis, version 1.32. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Canada

  • Zeller DC (2002) Tidal current orientation of Plectropomus leopardus (Serranidae). Coral Reefs 21:183–187

    Google Scholar 

  • Zhu ZY, Yue GH (2008) The complete mitochondrial genome of red grouper Plectropomus leopardus and its applications in identification of grouper species. Aquaculture 276:44–49

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by “Shuguang” fund from Shanghai Education Committee (09SG48), National Key Technology R&D Program 2011BAD13B01 from Ministry of Science and Technology, Chinese National Fund 40776089 and 31272662.

Author information

Authors and Affiliations

  1. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China

    Nanxi Liu, Siqi Li & Junbin Zhang

Authors
  1. Nanxi Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Siqi Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Junbin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Junbin Zhang.

Additional information

Nanxi Liu and Siqi Li contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, N., Li, S. & Zhang, J. Isolation and characterization of 16 polymorphic microsatellite loci in the leopard coral grouper Plectropomus leopardus . Conservation Genet Resour 5, 1067–1069 (2013). https://doi.org/10.1007/s12686-013-9980-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-013-9980-y

Keywords

Search

Navigation