SNP discovery in the marine gastropod Concholepas concholepas by high-throughput transcriptome sequencing

Abstract

Novel simple nucleotide polymorphisms (SNP) were identified using 454 transcriptome pyrosequencing in the snail Concholepas concholepas. From 2,991 contigs annotated with SNPs from De novo assembly, 7,069 putative polymorphisms were selected according their quality and functional annotation (gene ontology), of these, 18 SNPs were validated by HRM analysis using 50 individuals from two wild populations along the Chilean coast (29°S–71°W and 39°S–73°W). Herein, 9 loci evidenced Hardy–Weinberg equilibrium between both populations. F _st value between populations was estimated in 0.004, and F _is were calculated in 0.286 and 0.291 for North and South populations respectively. Here we carried out the first SNP mining in C. concholepas using pyrosequencing technology that promise to be useful for future conservation strategies of this overexploited marine snail species.