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Development and characterization of eighteen microsatellites for endangered fish Huso dauricus

  • Microsatellite Letters
  • Published:
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Abstract

The Kaluga (Huso dauricus) is one of two species of Acipenseriformes endemic to the Amur River basin. Due to overfishing and environmental pollution, this species has been assessed as critically endangered by IUCN. In this study, eighteen polymorphic microsatellite markers were developed from an enriched genomic library for H. dauricus and characterized in thirty-one individuals. The number of alleles per locus ranged from two to ten with an average of 5.056. The observed heterozygosity and the expected heterozygosity varied from 0.238 to 1.000 (mean = 0.780) and from 0.209 to 0.855 (mean = 0.661), respectively. These polymorphic loci will be valuable tools for the population genetic analysis and conservation of H. dauricus.

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References

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Acknowledgments

This study was supported by the National R&D Infrastructure and Facility Development Program of China.

Author information

Authors and Affiliations

  1. National Local Joint Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, China

    Lu Cuiyun, Yin Qianqian, Cheng Lei, Gu Ying, Li Chao & Sun Xiaowen

  2. Total Field of Liaoning Dahuofang Reservoir Aquaculture, Fushun, 113000, China

    Yin Qianqian

Authors
  1. Lu Cuiyun
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  2. Yin Qianqian
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  3. Cheng Lei
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  4. Gu Ying
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  5. Li Chao
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  6. Sun Xiaowen
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Corresponding author

Correspondence to Lu Cuiyun.

Appendix

Appendix

HLJHY02

>GTCGACGATCCTGCTGGAGATGGGAGAGTTCTTCCAGATACAGGTACAGCAGCAACGCCTTCTGCAGTGTGTGTGTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATAACTCCACTGGTCAGTGCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTGGGTTTGGGAAAATGCT

HLJHY04

>GTCGACGATCCGGGAGAAACTGGAGAATCTCAAGGTACAAACCGGATAGAAACACACTCTGACGCACACGCACACACACTGACAGACACACACACACACTGACACACACACACACACACACACACACACACACACACTGACACACACTGACACACACAGACAGTGACACACACACACACACACACACACACACACACACACACACACACACACACACAGAGAAAACAATGTTAAAGACCAGATCACACACCAGTAAGTACTGACCAGCCCAGACCCTGCGCTTTTGAGCAGATCGTCGAC

HLJHY14

>GTCGACGATCCTGTACGGATGGATGGACAAACTGACTTCTCCTGACACATAACAGGGGAGAAAGGCAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTGCCAGAGAGCGAGCCTCTGTGAGTGAGTGGAGGTGTACTGCATGCTGCTTTGTGTGAAAGGGCGCTGTCCACAACCGGGAGTGCTGAAAATCAAATACAAGACAGACTTTACTATCCTGTACAACCCAGGCAAGAAGAGGGAGAGGAGCAGAAAACCCAGCAAGACTTTACTGACCTGTACAACCCAGGCAAGGAGAGGGAGAGGAAAAGAAACCCGGCAAGACTTTAGTGACCTGTACAACCCAGGCAAGGAGAGGGAGAGGAGCAGAAACCCGGCAAGACTTTACAACCCAGGCAAGGAGAGGGAGAGGAGCAGAAACCCGGCAAGACTTTACTGACCTGTACAGCCCAGGCAAGGAGAGGGAGAGGAAAAGAAACCCGGCAAGACTTTACAACCCAGGCAAGGAGAGGGAGAGGAGCAGAAACCCGGCAAGACTTTGAAAAGAGCAGATCGTCGAC

HLJHY18

>GTCGACGACCATTCCATTCCCCGTAATAACGTGTTACTTCGCTGTCACGTCAAGACGTAATATTTTTTAACACGTCGCGGAAATCTGCCGAAGCTTCAGAACGGAGGGGCCTTAATCCTCGGCTATTTTCGCTGGCCAACGAGGCTGCTTGAAACGGGACTTCTTGCGCGCAGAATAGACTAAATTGCGATGCTGTTTTCTCTCTCGTTCTCTCTCTCTCTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGATGCTGAGGGGTGGGTGTT

HLJHY21

>GTCGACGATCATCATCATCACTCACACCAACCTGCAGGGTGAATACACACACACACACCCATAGCACCAACCTGCAGGGTGAATACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACATACACACACACACACACACACACACCTGTTTTCAATGCTTAGTACCATGAGCGAAGAAATTAAACCTAACAGAAGACTTGCTTTGAACATCTCCTCTAACCAAGATCGTCGAC

HLJHY22

>GTCGACGGTCAATATGACTATCCGAGTCAGAACAGAGTGACAGCAAATCAGAGTCTCCCTGGCATTCAACGTGAATCCAATGTTAGGAGCAGCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTGAGTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGTGTGTGTGGTGTGTGTGTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGGGCATGTATTACTATCTGTGTGTGTGTGTGTGTGAGAAAATGTCCTGCGAACAATTGAGTTATCTGTGAACAATGTCGCCGTGTTGTCCTTTTTTGTGCTGACCATAAACGACAGCTCATGTTCCTCTACGGTATCCAATTCTTGACTATCCTCCCCCTGCGGGGCGTGAAAGCTTGACTCTGGCCCTCTTTTTTACAACCTTCCCGATTTACAAC

HLJHY35

>CCGACTTGACATGATTACGATTCGAGCTCGGTACCCGGGGATCCTCTAGAGATTGTCAAGAATTCGGTACCGTCGACGATCAAACTGTATGAGAGCGTGTGTGTGCGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGCCTCGCTGTGTCACACGCCTGAACTGCTCAGCACAAAGACTGCTGGAGAGCAGGGAGAGCGTCTCTCTGGTTCTCCCGCGCGCGTGACGATGTCTAGAAATGTAACCACAAGGACTTGCTGGAGCCCA

HLJHY37

>GTCGACGATCCCTCCCCACCCCCGTGCACAATTTCAGCAGAAGTTGTAAAATGCAGCTCATTTAGTTCCGGATAGCGAGGATAGTATTTTTTTTTTACCACCTACAGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTGTGAGATGATCAGAGAGTTCCCTGACCTGCACCGCACCTTCGTGGCTGAGAGAGACATCTACCTCACCTACATGCTGAAACAGGCAACCCAAAAAGTGGAGCTCCCTGTACTGCAAGAGAGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGACTGTGTGCGACTTTTGTGACTCCGGGGGGGGGGCTGTGAGGA

HLJHY43

>GTCGACGATCTTGGGTCAGGGTAGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGAGTCCAGAATAAAAATTCGTGAGTGGTTCTGTGGAAATGAATGTATCAGTATATGAATACGCTAAATAATGACCCTCGTGTGAATAACCCCTTCCCTCAGTACAGCAGAAGAGTCAGACGTGACCCCCCTCAATATTTACGCACCCTCCCTCTTTACAAATCCTCTTACTCGACTCAACGATTGCCAATATGTTATTT

HLJHY47

>GTCGACGATCCTGCAGACACAAAAACAAGCATGTCACTTCCATACACCTCCTCCGCCAAGGCTAATCATGCAAAACCACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACATACACACACACACACACACAGACACAGACACATACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACTAATGCTAGGTACAATAATATCAGGTATGGGTTTTTAACATTGCATTCAGTTGTAACGCTCGCTTCATTAACTGCGCATGTCAGGTACAAAGGGTGTTCTAACTTCTATGTTTGACATCATTGATCGTCGACGTACCGAACTCGTGACATC

HLJHY53

>GTCGACGATCCAGGGCCTCGGGCGTACAATTTGAATGAAACGGTTCTTAAAAGAGAGACCCGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTCTGTGTGTGTGTGTGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGAGAGAGAGATAGATATAGAAAGATACTTTGAAAAGTCTTAAAAAGATTATCGACGGAACCAAATTCTTGACTATCTTAAACCTGCTCTGTGCAAGCTTGG

HLJHY61

>ATGACGTTGACATGATTACGATTCGAGCTCGGTACCCGGGGATCCTCTAGGGTGGGTCAAGAATTCGGTACCGTCGACGATCATGTGTGTGTGGTGTGTGTGTGTGTGTGTGTGTGTGAAACAGCCCTCCTCAGAGCTTACGCGTGTTTGAGAAGGAGGATAACCTTTTTACTAAACCAGCATCAATCCATTAATGGTGTATCGTCGAAGTACCGAATTCTTGGAAAGAATAATGGTGCCTGAATGGTATTTTCTCATTACGCATGTGTGCCGTATTTCACCCCATGAATGGTGCTCTCTTTGTAAACCT

HLJHY68

>GTCGACGATCATACTGCTGCTGTGTGTCCTGCTGCCTGGGACCGTGCTGCTGTACCAGGTAATGAAGGAGCCCTGATACACTGACACACACACACTGACACACACACACTAACACACACACGCGCACACACACACACACACACACGCGCACACACACATACACGCACACACACACACACACAGTCTGTAATATAAGCCAACAAATGTTCACACAAGGGACATCATAAATAATATCTTTTCAATCAGGAAAACCCTTTCAAATTAATTTTTCTATGTAGTCATCTACAGGCTGCAACAGACAAAGAATGGCCACTTCTATGCTCATTGAAGCTAGGGGGCCTGAGCCAGAGGGATCGTCGAC

HLJHY72

>GTCGACGATCAGCAGCCAGTACAGATGGAATATAGCGGGGTGATGTGATGTGTGCACGACTCCATCACTCACACACACACACACACACACACACACACACACACAGAGACACACCCACACACACACTGACACACACACACACACACACACACAGTGAGGATTATAGCGGGGTGATGTGATGTCTGCACGGCTCCAGAGCCACACACTGCATGCACGGCTGGACACACACACACACACACACACACACACACACACACACACACACACACACACCTTGTGGAATCACACTGGTTTGATGCAGTACAGGAAAGCAAAGCCGGAACCAGCCTACTCCATAGAGACAGCCCAGACGGATTCAGAACAGGTATGATCGTCGAC

HLJHY74

>GTCGACGATCCGCGCCGCCATGACGCCTAATGGTCCCCTGTGTCCGGTAGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATTTGAATCGGCAAGCAGGATTCGGGAGTGGATTTAGCACAATGGACCGTCTTCAAGTTGTACAGCATGTGATCGTCTAC

HLJHY75

>GTCGACGATCAGACCCAGCTGAAAGTCAGTAATGCTGAAACTTCCCCTGCTTGCTTCTACTACAGGTCCTGAGTTAGGAACACAGAACAGTAATCTTGTTGTAACCTCTGATTACACACACACACACACAGACACAGACACAGACACAGACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACGGCGCAAGTCTCTCCTGTTAATCTTCTCGTTGCAAGCCTATTCAAGGTGTGTGATTTCCTGTATATAAAGCATGCCTGCATGCAGATTTTGGCCTTGCATTCTTTAGCAATGAATCAGTTTGAAGTGGACCTCGAGGGCGTGCACTCTCTCAGGCTGCTGGGATACTCTCTTTCTCAGATCGTCGAC

HLJHY78

>GTCGACGATCAAGGAAATTGCATTGTAGAGTTCATGTGATGTGAGAAAAAAGGTAAACCAAGGACCTTCACACTTTTCACACACACACACAGAAACACACACACAGACACGCACACGCACACACACACACACACACACACACACACACACACACACACACACACACCGTTCAAGACAGTTTGGAAACTGCTTTTGGGATACTGTTTGAATATCACTATATTCCTCTGAACTCCAGCAGAACCCTAATAAGGCTACATCGTTACAAACAATGTCAACACAATAGATCGTCGAC

HLJHY89

>GTCGACGATCTGCACAGCGTTTGTTTAACGCTCTCCCAAGATGAATTACGCTCTTAGTTTGAATTTATTTTTTCAAATGTTGCATGTGCCGGTTCAGCGCTAGCGCAGTTCGAATTGTTATGAGTAAGTGTGCTCATGTTCATTTTATCTGATTCCACTTGCTAATAATGCATCTGAAATAGACAGACATGCTACACACACACACACACACACACACACACACACACACACACACACACACACACAGACTCTCTCCATTAGGGGCTGCCTGGGGGGGATCGTCGAC

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Cuiyun, L., Qianqian, Y., Lei, C. et al. Development and characterization of eighteen microsatellites for endangered fish Huso dauricus . Conservation Genet Resour 6, 361–365 (2014). https://doi.org/10.1007/s12686-013-0094-3

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  • DOI: https://doi.org/10.1007/s12686-013-0094-3

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