Abstract
Sixteen polymorphic, dinucleotide microsatellite loci were developed for Rhoadsia altipinna, a small characid fish from impacted rivers in south western Ecuador. None of the loci were in linkage disequilibrium or deviated significantly from Hardy–Weinberg equilibrium after sequential Bonferroni correction. Variability was relatively high with allelic richness ranging between 2 and 22 alleles per locus (average = 9.125), observed heterozygosity ranging between 0.125 and 0.958 (average = 0.695), and expected heterozygosity ranging between 0.120 and 0.952 (average = 0.736).
References
Barriga RS (2012) Lista de peces de agua dulce e intermareales del Ecuador. Revista Politécnica 30:83–119
Cardoso AR (2003) Subfamily Rhoadsiinae (Characins, tetras). In: Reis RE, Kullander SE, Ferraris CJ Jr (eds) Checklist of the freshwater fishes of South and Central America. Edipucrs, Porto Alegre, pp 213–214
Schuelke M (2000) An economic method for fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234
Vu V, Christman J, Calle P, Aguirre WE (2013) Isolation of microsatellite loci for the predatory fish Hoplias microlepis (Characiformes: Erythrinidae) from a highly impacted river system in western Ecuador. Conserv Genet Resour 5:437–439
Weitzman SH, Menezes NA, Britski HA (1986) Nematocharax venustus, a new genus and species of fish from the Rio Jequitinhonha, Minas Gerais, Brazil (Teleostei: Characidae). Proc Biol Soc Wash 99:335–346
Acknowledgments
Assistance by Freddy Villao, Margaret Silliker, Félix Man-Ging, Enrique Laaz, Sebastian Alvarado, Uma Dandekar, Alex Riegel, Mireya Pozo, and Mario Brito is gratefully acknowledged. Samples were exported from Ecuador under Permit 012-09 DR5-MAE. Funding was provided by DePaul University.
Author information
Authors and Affiliations
Corresponding author
Additional information
Maxine Loh and Webster Vital have contributed equally to this work.
Rights and permissions
About this article
Cite this article
Loh, M., Vital, W.F., Vu, V. et al. Isolation of sixteen microsatellite loci for Rhoadsia altipinna (Characiformes: Characidae) from an impacted river basin in western Ecuador. Conservation Genet Resour 6, 229–231 (2014). https://doi.org/10.1007/s12686-013-0062-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-013-0062-y