Skip to main content
SpringerLink
Log in

Isolation and characterization of microsatellite loci in the intertidal snail Batillaria multiformis (Mollusca Gastropoda)

  • Microsatellite Letters
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Intertidal snails of the genus Batillaria are a common group in tidelands throughout Japan. Recently, population sizes of Batillaria multiformis have been reduced due to coastal area reclamation. Using next generation sequencing, we developed 11 microsatellite markers to reveal the genetic diversity and gene flow among populations and combined them into a single multiplex panel. Evaluating polymorphisms in two populations showed that the numbers of alleles per locus ranged from 2 to 17 and 2 to 15, and the expected heterozygosity ranged from 0.274 to 0.936 and 0.455 to 0.934 in Mangokuura estuary and Kasari Bay, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc B 57:289–300

    Google Scholar 

  • Blacket MJ, Robin C, Good RT, Lee SF, Miller AD (2012) Universal primers for fluorescent labelling of PCR fragments—an efficient and cost-effective approach to genotyping by fluorescence. Mol Ecol Resour 12:456–463

    Article  CAS  PubMed  Google Scholar 

  • Furota T (2000) Extinction and conservation of molluscs in enclosed waters, a consideration from population decline in the batillariid snails in Tokyo Bay. Kaiyo Month 20:74–82 (in Japanese)

    Google Scholar 

  • Holleley CE, Geerts PG (2009) Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR. Biotechniques 46:511–517

    Article  CAS  PubMed  Google Scholar 

  • Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin JF (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26:403–404

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by the Interdisciplinary Collaborative Research Program of the Atmosphere and Ocean Research Institute at the University of Tokyo as well as a Sasakawa Scientific Research Grant from the Japan Science Society. We are also grateful to Junya Hirai, a PhD student in laboratory of Marine Planktology of the Atmosphere and Ocean Research Institute, the University of Tokyo, for supporting our research.

Author information

Authors and Affiliations

  1. Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba, 277-8564, Japan

    Hajime Itoh, Kimiko Hirose & Shigeaki Kojima

  2. Graduate School of Urban Engineering, Osaka City University, 3-3-138, Sugimoto, Sumiyoshi, Osaka, 558-8585, Japan

    Satomi Kamimura

Authors
  1. Hajime Itoh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Satomi Kamimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kimiko Hirose
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shigeaki Kojima
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hajime Itoh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Itoh, H., Kamimura, S., Hirose, K. et al. Isolation and characterization of microsatellite loci in the intertidal snail Batillaria multiformis (Mollusca Gastropoda). Conservation Genet Resour 6, 193–195 (2014). https://doi.org/10.1007/s12686-013-0046-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-013-0046-y

Keywords

Search

Navigation