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Isolation and characterization of fifteen microsatellite loci in Leopard grouper (Mycteroperca rosacea) via 454 pyrosequencing

  • Microsatellite Letters
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Abstract

Leopard grouper (Mycteroperca rosacea) are distributed from Bahía Magdalena, through the Gulf of California and as far south as Jalisco, Mexico. They are listed as Vulnerable on the IUCN Red List due to significant fishing-induced declines over the past decade. Microsatellite loci were isolated by constructing a shotgun genomic library and sequencing using 454 XL + titanium chemistry. We characterized 15 polymorphic loci in 120 samples from three geographic locations. Genotyping yielded 15–60 alleles per locus with observed levels of heterozygosity ranging from 0.575 to 0.975.

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References

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Acknowledgments

Funding for this study was provided by National Geographic (Young Explorers Grant #8928-11), the PANGAS project of the David and Lucille Packard Foundation, a UC-HBCU Initiative Grant, and a Mia J. Tegner Memorial Research Grant. Scientific collection permits were issued by SAGARPA (No. DGOPA.09151.260809.2885 and DAPA/2/020511/01197). This is a scientific contribution to the PANGAS project (http://pangas.arizona.edu).

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Authors and Affiliations

  1. Department of Ecology and Evolutionary Biology, University of California Santa Cruz, 100 Shaffer Road, Santa Cruz, CA, 95060, USA

    Alexis M. Jackson, Avian S. Lain, Aneese J. Williams & Giacomo Bernardi

  2. School of Natural Resources and the Environment, University of Arizona, 1131 E. 4th Street, Tucson, AZ, 85721, USA

    Adrian Munguía-Vega

  3. Comunidad y Biodiversidad A.C., Isla del Peruano 215, Lomas de Miramar, Guaymas, Sonora, 85448, Mexico

    Adrian Munguía-Vega

  4. Department of Chemical Engineering, Hampton University, 100 E. Queen Street, Hampton, VA, 23668, USA

    Avian S. Lain

  5. Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA, 95064, USA

    Simon A. Stokes

  6. Department of Environmental and Marine Science, Hampton University, 100 E. Queen Street, Hampton, VA, 23668, USA

    Aneese J. Williams

Authors
  1. Alexis M. Jackson
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  2. Adrian Munguía-Vega
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  3. Avian S. Lain
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  4. Simon A. Stokes
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  5. Aneese J. Williams
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  6. Giacomo Bernardi
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Corresponding author

Correspondence to Alexis M. Jackson.

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Jackson, A.M., Munguía-Vega, A., Lain, A.S. et al. Isolation and characterization of fifteen microsatellite loci in Leopard grouper (Mycteroperca rosacea) via 454 pyrosequencing. Conservation Genet Resour 6, 185–187 (2014). https://doi.org/10.1007/s12686-013-0044-0

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  • DOI: https://doi.org/10.1007/s12686-013-0044-0

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