Abstract
The critically endangered western black crested gibbon (Nomascus concolor) lives only in isolated mountainous areas of China’s Yunnan province, northern Vietnam, and northwestern Laos. A lack of genetic samples and polymorphic molecular loci has limited our ability to investigate the genetic diversity of this species. We collected fecal samples from 12 free-ranging individuals in the Wuliang Mountains of Yunnan and developed 13 polymorphic microsatellite loci using 1/8 plate of a Roche 454 GS-FLX sequencing run. The number of alleles ranged from three to eight per locus, and the observed and expected heterozygosities were 0.333–0.917 and 0.424–0.822, respectively. These loci will be useful tools not only for future studies of the social structure and population genetics of N. concolor, but also will facilitate an informed conservation management plan.
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Acknowledgments
This study was supported by the National Natural Science Foundation of China (31070349, 31170498), Yunnan Provincial (2011FB105), Chinese Academy of Science (KSCX2-EW-Z-4-1, GREKF11-04), the National Science Foundation (NSF OISE-1015770, NSF BCS-1155904), The Leakey Foundation, The American Philosophical Society Lewis and Clark Fund, Lambda Alpha, and Washington University in St. Louis. Thanks are given to The Key Lab of Animal Ecology and Conservation Biology, Institute of Zoology, The Chinese Academy of Sciences, for technical guidance. Thanks to Douglas Yu, Ji Yinqiu, and Yang Yahan for valuable advice and assistance, and Alan Templeton for commenting on a draft of this paper. Special thanks to Fan Pengfei, Sun Guozheng, Guan Zhenhua, Ni Qingyong, Wang Xiaowei and Ning Wenhe for collecting samples. We thank the staff of the Jingdong Nature Reserve Management Bureaus for their much-needed support.
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Hu Naiqing and Joseph Orkin have contributed equally to this work.
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Hu, N., Joseph, O., Huang, B. et al. Isolation and characterization of thirteen microsatellite loci for the western black crested gibbon (Nomascus concolor) by high-throughput sequencing. Conservation Genet Resour 6, 179–181 (2014). https://doi.org/10.1007/s12686-013-0041-3
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DOI: https://doi.org/10.1007/s12686-013-0041-3