Abstract
We developed microsatellite markers for the recently described forest rodent, Eliurus carletoni, from an enriched genomic library. Nine loci composed of four dinucleotide, one trinucleotide, one tetranucleotide and three compound repeats were isolated and characterized using two wild populations. One locus was found to be monomorphic. For the polymorphic loci, the average number of alleles per locus was 7.13 and 8.38 for each population. Mean expected and observed heterozygosities were high (i.e. 0.76 and 0.80). Tests for linkage disequilibrium were not significant across all locus pairs. One locus tested significant for null alleles, but only one population exhibited a significant deviation from the Hardy–Weinberg Equilibrium (HWE) at this locus. All remaining loci show no evidence of departure from HWE. Overall, we identified eight polymorphic loci that may be used in conservation and population genetics studies of E. carletoni.
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Acknowledgments
Tissue samples were provided by Martin Raheriarisena and Steven M. Goodman. J.E.R would like to thank Adalgisa Caccone and Jeff Powell for their supports in the laboratory. The isolation and characterization of the microsatellite loci presented herein were carried out at the Molecular Systematics and Conservation Genetics Laboratory at Yale University with funding supports from the Yale Institute for Biospheric Studies in addition to grants to J-.E.R. by the Society for Integrative and Comparative Biology. The screening of the variability of the selected loci in wild populations was carried out at the Conservation Genetics Department of Omaha’s Henry Doorly Zoo with the support of Bill and Berniece Grewcock.
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Rakotoarisoa, JE., Bailey, C.A., Hinger, P.H. et al. Isolation and characterization of nine microsatellite loci in a Malagasy endemic rodent, Eliurus carletoni (Rodentia: Nesomyinae). Conservation Genet Resour 5, 203–205 (2013). https://doi.org/10.1007/s12686-012-9768-5
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DOI: https://doi.org/10.1007/s12686-012-9768-5