Abstract
Lumpfish (Cyclopterus lumpus L. 1758) are widely distributed on both sides of the North Atlantic. They are a commercially important species, but stock size estimates have declined since the mid-1980s in Canada, Norway and Iceland. Little is known about the biology of this species, in particular the breeding migrations and population structure which are fundamental for effective management. This paper describes the development and characterization of twenty-two polymorphic microsatellite loci using next generation sequencing. The number of alleles per locus ranged from 3 to 27 in two geographically distant North Atlantic populations, with observed and expected heterozygosities ranging between 0.0625–0.979 and 0.0618–0.946, respectively. These loci are an important resource that will allow assessment of the population genetic structure of this species, and contribute to its appropriate management.
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Acknowledgments
The authors thank Monty Way and Alain Fréchet for providing the Canadian samples and Halldór Gunnar Ólafsson at BioPol and the Icelandic lumpfish harvesters for their cooperation and support in our sampling efforts. We thank Alexandra M. Klonowski, Ágústa Sigfúsdóttir, Steinunn Magnúsdóttir and Snædís Björnsdóttir for their assistance in the laboratory. This research was supported by The Fisheries Research Fund of the Icelandic Ministry of Fisheries and Agriculture.
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Skirnisdottir, S., Olafsdottir, G., Olafsson, K. et al. Twenty-two novel microsatellite loci for lumpfish (Cyclopterus lumpus). Conservation Genet Resour 5, 177–179 (2013). https://doi.org/10.1007/s12686-012-9762-y
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DOI: https://doi.org/10.1007/s12686-012-9762-y