Abstract
The seaside sparrow (Ammodramus maritimus; SESP) includes seven extant subspecies ranging from the northeastern coast of the United States to the Gulf of Mexico. Genetic diversity of SESP may be impacted since coastal marsh habitats are highly fragmented, thereby limiting gene flow among adjacent populations. Genetic analysis of SESP populations will provide insight for development of conservation and management strategies for the species. We describe development and characterization of 11 novel microsatellite markers for SESP. These loci will be useful for individual identification as well as studies of fine-scale genetic relatedness within populations and subspecies of SESP.
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References
Avise JC, Nelson WS (1989) Molecular genetic relationships of the extinct dusky seaside sparrow. Science 243:646–648
Boutin-Ganache I, Raposo M, Raymond M, Deschepper CF (2001) M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods. Biotechniques 31:24–28
Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (1991) Touchdown PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19:4008
Faircloth BC (2006) GMCONVERT: file conversion for GENEMAPPER output files. Mol Ecol Notes 6:968–970
Faircloth B (2008) MSTACOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. In: Zimmer EA, Roalson EH (eds) Methods in enzymology 395, Molecular evolution: producing the biochemical data, part B. Academic Press, San Diego
Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error and increases success in paternity assignment. Mol Ecol 16:1099–1106
Post W, Greenlaw JS (2009) Seaside sparrow (Ammodramus maritimus). In: Poole A (ed) The birds of North America online. Cornell Lab of Ornithology, Ithaca
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Heredity 86:248–249
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Rosen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
US Department of the Interior (2011) Partial 90-day finding on a petition to list 404 species in the Southeastern United States as endangered or threatened with critical habitat (Proposed Rule). Federal Register 76(187):59836–59862
US Fish and Wildlife Service (2010) Species profile: cape sable seaside sparrow (Ammodramus maritimus mirabilis). US Fish and Wildlife Service, Atlanta
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Lehmicke, A.J.J., Berry, B.E., Shamblin, B.M. et al. Isolation and characterization of tetranucleotide microsatellite loci from the seaside sparrow (Ammodramus maritimus). Conservation Genet Resour 4, 881–884 (2012). https://doi.org/10.1007/s12686-012-9664-z
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DOI: https://doi.org/10.1007/s12686-012-9664-z