Abstract
The roughskin sculpin (Trachidermus fasciatus) is an endangered species. It has been listed as an “endangered aquatic wild animal under second class protection” since late 1980s in China. In this study, 19 polymorphic microsatellite loci were isolated and characterized. The markers were tested on 60 individuals collected from Yalu River (YL) and the mouth of Yellow River (YR) in China. The number of alleles in these 19 markers ranged from 10 to 20. The observed (H O) and expected (H E) heterozygosity ranged from 0.8000 to 1.000 in YL and from 0.8638 to 0.9497 in YR, respectively. The polymorphism information content varied from 0.8230 to 0.9300 in the two populations. These microsatellite loci could help in genetic studies on this species and may provide essential information for its conservation.
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Acknowledgments
This research was supported by Leading Academic Discipline Project of Shanghai Municipal Education Commission (marine biology J50701), Shanghai Aquatic biology Leading Academic Discipline Project (S30701), Shanghai Invigorate Agriculture though Science and Technology Advances Project (2006-3-5) and Shanghai Leading Academic Discipline Project (Y1101). We gratefully thank professor Tang Zuo Peng (Liaoning province) and Mr. Zhou Hui (Zhejiang province) for the help in sample collection.
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Liu, ZZ., Zeng, Z., Pan, LD. et al. Isolation and characterization of polymorphic microsatellite markers for the endangered roughskin sculpin (Trachidermus fasciatus). Conservation Genet Resour 4, 837–840 (2012). https://doi.org/10.1007/s12686-012-9654-1
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DOI: https://doi.org/10.1007/s12686-012-9654-1