Abstract
Thirty polymorphic microsatellite markers for swimming crab P. trituberculatus are developed from EST sequences, genomic libraries and cross-species amplification in P. pelagicus, respectively. They are characterized using 24 P. trituberculatus individuals from Yingkou natural population. The number of alleles per locus range from 4 to 21 with a mean number of 10.83. The observed and expected heterozygosities range from 0.250 to 1.000 and from 0.365 to 0.945, respectively. Five loci departed significantly from HWE (P < 0.05). The genomic-SSRs and EST-SSRs show similar levels of polymorphism for both the average number of alleles (11 alleles vs. 10 alleles) and average PIC values (PIC = 0.780 vs. PIC = 0.776). These microsatellite loci from different sources will contribute to improve research on the genetic conservation of P. trituberculatus.
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Acknowledgments
This research was supported by Chinese National ‘863’ Project under Grant No. 2006AA10A406 and foundations from the National Natural Science Foundation of China (40676085 and 40976088).
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Cui, Z., Liu, Y., Wang, H. et al. Isolation and characterization of microsatellites in Portunus trituberculatus . Conservation Genet Resour 4, 251–255 (2012). https://doi.org/10.1007/s12686-011-9518-0
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DOI: https://doi.org/10.1007/s12686-011-9518-0