Abstract
Eight microsatellite loci were isolated and characterized from Glyptothorax laosensis using an enriched genomic library method. The polymorphism of these markers was determined from 39 individuals. The number of alleles per locus ranged from 2 to 7. The observed and expected heterozygosity ranged from 0.7407 to 0.9407 and from 0.8835 to 0.9079, respectively. The eight polymorphic markers would be used to study the genetic diversity and genetic structure of the G. Laosensis.
References
Aljanabi SM, Martinez I (1997) Universal and salt-extracted of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res 25:4692–4693
Chu XL, Chen YR (1990) The fishes of Yunnan, China: PartII. Science Press, Beijing
Peng Z, He S, Zhang Y (2007) Phylogenetic relationships of glyptosternoid fishes (Siluriformes: Sisoridae) inferred from mitochondrial cytochrome b gene sequences. Mol Phylogenet Evol 31(3):979–987
Rozen S, Skaletsky H (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humama Press, Totowa, pp 365–386
Vos P, Hogers R, Bleeker M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
Yeh FC, Yang R, Boyle TJ, Ye Z, Xiyan JM (2000) POPGENE 32: Microsoft Windows-based freeware for population genetic analysis. 1.32. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton
Zane L, Bargelloni L, Patamello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16
Zhou W, Li MH (2006) The study on relationship between biodiversity and habitat of catfish family Sisoridae (Siluriformes) in China. J Yunnan Agric Univ 21:811–830
Acknowledgments
We thank Professor Guan Pin Yang, Doctor Li Nan Zhang and Peng Zhang from Ocean University of China for the help of technology. This study was supported by Special Fund for Agro-Scientific Research in the Public Interest (200903048) and the project of China Environmental Protection Agency (S10-2-12).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jin, J., Wang, D., Liu, M. et al. Isolation and characterization of eight microsatellite loci of Glyptothorax laosensis in the Lancang river. Conservation Genet Resour 4, 35–37 (2012). https://doi.org/10.1007/s12686-011-9467-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-011-9467-7