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Characterization of thirteen new polymorphic microsatellite markers from the honeycomb grouper Epinephelus merra

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Abstract

Epinephelus merra (Serranidae) is the most common and widespread species of this genus, mostly found in reef habitats from South Africa to Central Pacific. Thirteen polymorphic microsatellite loci were isolated and characterized from this species. Polymorphism was studied in two localities from the Comoros archipelago—Mohéli and Moroni. Allelic diversity was 7.5, polymorphism ranging from 3 to 13 alleles. Overall heterozygosity was high with an average observed heterozygosity of 0.587. Three of these loci were characterised by heterozygote deficiencies for both populations. Finally, ten of the 13 developed primers could be used in future population genetics study of E.merra that might be useful in a context of marine biodiversity conservation.

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Acknowledgments

This research was supported by European Funds—the European Cooperation Funds EU POCT-OI 2007-2013 (project CAMP) and the EU 7th Funding Program—Capacities, Research Potential (project RUNSeaSciences)-, the French State (DIREN), La Réunion Council (Région Réunion) and a MASMA grant of the WIOMSA. We acknowledge the authorities of Comoros for allowing our sample collection. We are indebted to Stephanie Ferreira and Stéphanie Duthoy from GenoScreen, France (www.genoscreen.fr) for their work and their permanent help.

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Correspondence to Delphine Muths.

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Muths, D., Bourjea, J. Characterization of thirteen new polymorphic microsatellite markers from the honeycomb grouper Epinephelus merra . Conservation Genet Resour 3, 629–631 (2011). https://doi.org/10.1007/s12686-011-9420-9

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  • DOI: https://doi.org/10.1007/s12686-011-9420-9

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