Abstract
Frogs in the genus Indirana are endemic to Western Ghats biodiversity hotspot. The species are poorly studied and in many cases threatened or endangered. Here we describe primers and polymerase chain reactions for 62 microsatellite loci for Indirana beddomii, one of the commonest frogs in the genus. Fifty-six of the primers were polymorphic on sample of 23 individuals from a single sampling site (Ponmudi, Kerala) with an average 9.11 alleles per locus (range = 2–20). The average observed and expected heterozygosities were 0.64 and 0.71, respectively. The loci should be useful in conservation genetic studies of Indirana frogs.
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Acknowledgments
We thank Marika Karjalainen and Kirsi Kähkönen for assistance in laboratory, Jacquelin DeFaveri for comments, Kerala forest department for sampling permissions and all the people who helped in obtaining the samples. The samples were collected under licence from National Biodiversity Authority, India (licence #: NBA/TECH Appl/9/85/34/08/08-09/682). Our research was funded by Academy of Finland (grant #: 125092) and Indian Department of Biotechnology (BT/IN/Finnish/11/SG/2008).
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Nair, A., Kumar, K.S., George, S. et al. Sixty-two new microsatellite markers for an endemic frog Indirana beddomii from the Western Ghats biodiversity hotspot. Conservation Genet Resour 3, 167–171 (2011). https://doi.org/10.1007/s12686-010-9315-1
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DOI: https://doi.org/10.1007/s12686-010-9315-1