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Effect of Chitosan-Coated Nanostructured Lipid Carrier on Escherichia coli Biofilms

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Abstract

Antimicrobial delivery systems are useful tools to control biofilm growth on abiotic surfaces such as urinary catheter. The present study examined whether nanostructured lipid carriers coated with chitosan (NLC-chitosan) affected the growth of uropathogenic biofilms of Escherichia coli. NLC-chitosan was prepared using the emulsion and sonication method, and further characterized with respect to particle size, polydispersity index, and zeta potential. After determining the minimum inhibitory and bactericidal concentrations, E. coli biofilms were grown on catheter specimens (following a preliminary study in which E. coli was found to adhere better to the catheter surface than glass slide and plate). At the 48, 72, 96, and 120 h of growth, they were exposed to 0.9% NaCl solution (negative control), 0.12% chlorhexidine solution (positive control), or NLC-chitosan (final chitosan concentration of 0.28%). After 24 h of treatment, the biofilms were collected to analyze their bacterial viability. NLC-chitosan preparation had bimodal particle size distribution with mean size of 292.9 ± 2.5 nm and polydispersity index of 0.24 ± 0.03 and positive zeta potential (+19.1 ± 0.2) indicating the nanoparticle coating by chitosan. Compared with the control groups, NLC-chitosan affected bacterial viability of biofilms at all ages studied (p<0.05). NLC-chitosan can effectively control the growth of young and mature biofilms of uropathogenic E. coli.

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Acknowledgements

We thank Dr Marcelo C. Alves for helping us to perform the statistical analysis, Dr Luciana Kabeya for the English corrections, and Mr. Alcides Silva Pereira for his technical assistance.

Funding

This study was supported by the Brazilian funding agencies São Paulo Research Foundation (FAPESP; grant no. 2015/17712-9) and Coordination for the Improvement of Higher Education Personnel (CAPES; Finance Code 001).

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Authors and Affiliations

  1. Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Sao Paulo, Ribeirão Preto, Brazil

    Michael Oluwole Osungunna, Ana Carolina S. Ré, Ana Cristina M. Polizello & Carolina Patrícia Aires

  2. Department of Pharmaceutics, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, 220005, Nigeria

    Michael Oluwole Osungunna

  3. Department of Clinical Analysis, Toxicology and Food Science, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Sao Paulo, Ribeirão Preto, Brazil

    André Pitondo-Silva

  4. Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Sao Paulo, Ribeirão Preto, Brazil

    Letícia Bueno Silva, Priscyla D. Marcato & Taís Nader Chrysostomo Massaro

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  1. Michael Oluwole Osungunna
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Correspondence to Michael Oluwole Osungunna.

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Osungunna, M.O., Pitondo-Silva, A., Silva, L.B. et al. Effect of Chitosan-Coated Nanostructured Lipid Carrier on Escherichia coli Biofilms. BioNanoSci. 11, 762–769 (2021). https://doi.org/10.1007/s12668-021-00872-z

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