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Characterization and FPLC Analysis of Garbage Enzyme: Biocatalytic and Antimicrobial Activity

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Abstract

Fermentation of fruit and vegetable peels produces garbage enzyme (GE) which was used to obtain industrially beneficial products. This study was conducted for the characterization of GE and evaluation of its biocatalytic and antimicrobial activity. The maximum activity of amylase, protease and lipase were 1.782 units/mg, 0.050 units/mg and 0.355 units/mg respectively were found in optimum conditions which also showed antioxidant properties. The enzyme solution was separated by Fast Protein Liquid Chromatography (FPLC) and experiments were conducted for antimicrobial activity with the FPLC generated fractions of GE on both gram positive and gram negative bacteria. Inhibition zones were measured from concentration dependent bioassay analysis of lyophilized FPLC fractions. FPLC fraction II offered best zone of inhibition in Escherichia coli, Staphylococcus aureus and in Rhizobium leguminosarum. Molecular weight of above fraction was determined by SDS-PAGE technique. Significant research findings of this research work will help in developing newer potential commercializable antimicrobial agents from GE generated from fruit and vegetable waste.

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Acknowledgements

The authors express thankfulness to Biotech Consortium India Limited (BCIL), a Department of Biotechnology (DBT) enterprise, Government of India (GoI) for granting financial support to conduct this part of research work under BCIL-Biotechnology Industrial Training Program, North Eastern States NES program (BITP-NES) programme. Special thanks offered to the authorities of Guwahati Biotech Park, Govt. of Assam (GOA) for hosting the BCIL-BITP NES programme.

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Author notes

  1. Samina Rahman and Irshadul Haque have contributed equally to this work.

Authors and Affiliations

  1. Guwahati Biotech Park, Technology Complex, IIT Guwahati, Kamrup, 781039, India

    Samina Rahman, Irshadul Haque, Prajjalendra Barooah & Bula Choudhury

  2. Centre for Biotechnology and Bioinformatics, Dibrugarh University, Dibrugarh, 786004, India

    Rajiv C. Dev Goswami & Kaushal Sood

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  1. Samina Rahman
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  2. Irshadul Haque
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  3. Rajiv C. Dev Goswami
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  4. Prajjalendra Barooah
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  6. Bula Choudhury
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Correspondence to Bula Choudhury.

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Rahman, S., Haque, I., Goswami, R.C.D. et al. Characterization and FPLC Analysis of Garbage Enzyme: Biocatalytic and Antimicrobial Activity. Waste Biomass Valor 12, 293–302 (2021). https://doi.org/10.1007/s12649-020-00956-z

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  • DOI: https://doi.org/10.1007/s12649-020-00956-z

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