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Antioxidant and Enzyme Inhibitory Activities of Metapenaeus monoceros By-Product Hydrolysates Elaborated by Purified Alkaline Proteases

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Abstract

This study investigated the antioxidant potential and enzymatic inhibitory power of shrimp by-product protein hydrolysates (hyd) prepared using two purified serine alkaline proteases namely; alkaline protease from Anoxybacillus kamchatkensis strain M1V (SAPA) and alkaline protease from Aeribacillus pallidus strain VP3 (SPVP). The characterization of their molecular weights showed the presence of peptides less than 15 kDa. SAPA-hyd displayed the highest reducing power activity. Likewise, SAPA-hyd was found to be active against angiotensin I-converting enzyme (ACE), tyrosinase (Tyr), and amylase (Amy) enzymes with IC50 values of 10.01, 6.13, and 4.11 µg/mL, respectively. SPVP-hyd revealed the highest radical scavenging power, β-carotene protection, and ferrous chelating activity with EC50 values of 11.79, 3.69, and 0.61 µg/mL, respectively. After fraction nation, all fractions displayed a high inhibitory potential against ACE. Only fractions F2 and F3 from SAPA-hyd showed the highest anti-tyrosinase activity with IC50 values of 25.10 and 12.5 ng/mL, respectively. These results suggest that a simple, clean, economic, and controllable bioprocess for the utilization of shrimp by-product was investigated. This study gives an unprecedented report on the anti-Tyr activity of the protein hydrolysate from fish bio-waste. Additionally, two peptide hydrolysates with multifunctional biological activities were elaborated from Metapenaeus monoceros by two bacterial purified proteases.

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Acknowledgements

The authors would like to thank Mrs. M. Mezghani Abid and Mr. A. Hadj Brahim (LMBEE-CBS) and Mr. I. Hssairi, Mr. A. Zitoun, and Mr. F. Boukhili (UVRR-CBS) for their technical assistance. The authors would also like to express their gratitude to Mr. K. Walha, and Mrs. N. Masmoudi (Analysis Unit-CBS) for their technical assistance. Special thanks are also due to Dr. Z. Bouallagui (LBPE-CBS) and to Pr. W. Hariz from the English Department at the Sfax Faculty of Science, University of Sfax (Sfax, Tunisia) for constructive proofreading and language polishing services.

Funding

This study was supported by the Ministry of Higher Education and Scientific Research, Tunisia, under the Contract Program 2015–2019: Grant No. LMBEE_CBS/code: LR15CBS06 and the Tunisian-Algerian project JAOUADI/BADIS_TNDZ-MicrooZymes_2012-2018/code: TA/04/2012.

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Authors and Affiliations

  1. Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia

    Sondes Mechri, Khelifa Bouacem, Fadoua Jabeur & Bassem Jaouadi

  2. Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia

    Imen Sellem

  3. Laboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences (FSB), University of Sciences and Technology of Houari Boumediene (USTHB), P.O. Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria

    Khelifa Bouacem, Hocine Hacene & Amel Bouanane-Darenfed

  4. Laboratory of Environmental Bioprocesses (LEBP), LMI COSYS-Med, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia

    Mohamed Chamkha

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  1. Sondes Mechri
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Mechri, S., Sellem, I., Bouacem, K. et al. Antioxidant and Enzyme Inhibitory Activities of Metapenaeus monoceros By-Product Hydrolysates Elaborated by Purified Alkaline Proteases. Waste Biomass Valor 11, 6741–6755 (2020). https://doi.org/10.1007/s12649-020-00942-5

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