Skip to main content
SpringerLink
Log in

Valorization of Palm Oil Mill Effluent into Lipid and Cell-Bound Lipase by Marine Yeast Yarrowia lipolytica and Their Application in Biodiesel Production

  • Original Paper
  • Published:
Waste and Biomass Valorization Aims and scope Submit manuscript

Abstract

Purpose

This study aimed to valorize palm oil mill effluent (POME) into lipid and lipase by marine yeast Yarrowia lipolytica and to evaluate their application in biodiesel production.

Methods

Several strains of Yarrowia lipolytica were cultivated in POME and the most promising strain was selected and its culture conditions were optimized. After optimization, the yeast cells were harvested and its cell-bound lipase was characterized. Finally, both yeast lipid and cell-bound lipase were applied for biodiesel production through direct transesterification without cell drying and lipid extraction steps.

Results

All yeast strains grew well on POME and accumulated lipid >33 % but only Y. lipolytica TISTR 5151 possessed high activity of cell-bound lipase (610 ± 87 U/L). After process optimization, the selected Y. lipolytica TISTR 5151 produced lipid and cell-bound lipase at the highest levels of 1.64 ± 0.03 g/L and 3353 ± 27 U/L, respectively. In the direct transesterification reaction, the cell-bound lipase could transesterify lipid from the wet yeast cells and produced 40.9 % of fatty acid methyl esters.

Conclusions

The ability of this yeast for valorization of POME into lipid and lipase and its possible application in direct transesterification process, may contribute greatly to economical and environment-friendly biodiesel production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Tongboriboon, K., Cheirsilp, B., H-Kittikun, A.: Mixed lipases for efficient enzymatic synthesis of biodiesel from used palm oil and ethanol in a solvent-free system. J. Mol. Catal. B Enzym. 67, 52–59 (2010)

    Article  Google Scholar 

  2. Zhu, L.Y., Zong, M.H., Wu, H.: Efficient lipid production with Trichosporon fermentans and its use for biodiesel preparation. Bioresour. Technol. 99, 7881–7885 (2008)

    Article  Google Scholar 

  3. Papanikolaou, S., Aggelis, G.: Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single-stage continuous culture. Bioresour. Technol. 82, 43–49 (2002)

    Article  Google Scholar 

  4. Xue, F., Miao, J., Zhang, X., Luo, H., Tan, T.: Studies on lipid production by Rhodotorula glutinis fermentation using monosodium glutamate wastewater as culture medium. Bioresour. Technol. 99, 5923–5927 (2008)

    Article  Google Scholar 

  5. Saenge, C., Cheirsilp, B., Suksaroge, T., Bourtoom, T.: Potential use of oleaginous red yeast Rhodotorula glutinis for the bioconversion of crude glycerol from biodiesel plant to lipids and carotenoids. Process Biochem. 46, 210–218 (2011)

    Article  Google Scholar 

  6. Guerzoni, M.E., Lanciotti, R., Vannini, L., Galgano, F., Favati, F., Gardini, F., Suzzi, G.: Variability of lipolytic activity in Yarrowia lipolytica and its dependence on environmental conditions. Int. J. Food Microbiol. 69, 79–89 (2001)

    Article  Google Scholar 

  7. Papanikolaou, S., Chevalot, I., Galiotou-Panayotou, M., Komaitis, M., Marc, I., Aggelis, G.: Industrial derivative of tallow: a promising renewable substrate for microbial lipid, single-cell protein and lipase production by Yarrowia lipolytica. Electron. J. Biotechnol. 10, 425–435 (2007)

    Article  Google Scholar 

  8. Wang, L., Chi, Z., Wang, X., Liu, Z., Li, J.: Diversity of lipase producing yeasts from marine environments and oil hydrolysis by their crude enzymes. Ann. Microbiol. 57(4), 495–501 (2007)

    Article  Google Scholar 

  9. Jong, B.C., Liew, P.W., Lebai, J.M., Kim, B.H., Mohd Dzomir, A.Z., Leo, K.W., Awang, M.R.: Performance and microbial diversity of palm oil mill effluent microbial fuel cell. Lett. Appl. Microbiol. 53, 660–667 (2011)

    Article  Google Scholar 

  10. Mustar, S., Mohd, M.A., Noor., Ahmad, R., Amin, A.M.: Single cell protein from palm oil mill effluent. In: Proceedings of NSF Workshop, Kuala Lumpur (2001)

  11. APHA, AWWA, WPCF: Standard Method of the Examination of the Water and Wastewater, 20th edn. American Public Health Association, Washington, DC (2001)

    Google Scholar 

  12. Folch, J., Lees, M., Stanley, G.H.S.: A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226, 497–509 (1957)

    Google Scholar 

  13. Lee, S., Rhee, J.S.: Production and partial purification of lipase from Pseudomonas putida 3SK. Enzyme Microb. Technol. 15, 617–623 (1993)

    Article  Google Scholar 

  14. Kaewthong, W., H-Kittikun, A.: Glycerolysis of palm olein by immobilized lipase PS in organic solvents. Enzyme Microb. Technol. 35, 218–222 (2004)

    Article  Google Scholar 

  15. Sharma, R., Chisti, Y., Banerjee, U.C.: Production, purification, characterization and applications of lipase. Biotechnol. Adv. 19, 627–662 (2011)

    Article  Google Scholar 

  16. Dalmau, E., Montesinos, J.L., Lotti, M., Casas, C.: Effect of different carbon sources on lipase production by Candida rugosa. Enzyme Microb. Technol. 26, 657–663 (2000)

    Article  Google Scholar 

  17. Hlavsova, C., Vollaro, L.: The use of Yarrowia lipolytica to reduce pollution in olive mill wastewaters. Water Res. 31(10), 2520–2524 (1997)

    Article  Google Scholar 

  18. Kitcha, S., Cheirsilp, B.: Enhancing lipid production from crude glycerol by newly isolated oleaginous yeasts: strain selection, process optimization and fed-batch strategy. Bioenergy Res. 6, 300–310 (2013)

    Article  Google Scholar 

  19. Yadav, K.N.S., Adsul, M.G., Bastawde, K.B., Jadhav, D.D., Thulasiram, H.V., Gokhale, D.V.: Differential induction, purification and characterization of cold active lipase from Yarrowia lipolytica NCIM 3639. Bioresour. Technol. 102, 10663–10670 (2011)

    Article  Google Scholar 

  20. Vakhlu, J., Kour, A.: Yeast lipases: enzyme purification, biochemical properties and gene cloning. Electron. J. Biotechnol. 9(1), 69–85 (2006)

    Article  Google Scholar 

  21. Wei, D., Zhang, L., Song, Q.: Studies on a novel carbon source and cosolvent for lipase production by Candida rugosa. J. Ind. Microbiol. Biotechnol. 31, 133–136 (2004)

    Article  Google Scholar 

  22. Pereira-Meirelles, F.V., Rocha-Leao, M.H.M., Sant Anna Jr, G.L.: Lipase location in Yarrowia lipolytica cells. Biotechnol. Lett. 22, 71–75 (2000)

    Article  Google Scholar 

  23. Fickers, P., Benetti, P.H., Wache, Y., Marty, A., Mauersberger, S., Smit, M.S., Nicaud, J.M.: Hydrophobic substrate utilization by the yeast Yarrowia lipolytica and its potential application. FEMS Yeast Res. 5, 527–543 (2005)

    Article  Google Scholar 

  24. Kalisz, H.M., Hendle, J., Schmid, R.D.: Structural and biochemical properties of glycosylated and deglucosylated glucose oxidase from Penicillium amagasakiense. Appl. Microbiol. Biotechnol. 47, 502–507 (1997)

    Article  Google Scholar 

  25. Ugwu, S.O., Apte, S.P.: The effect of buffers on protein conformational stability. Pharm. Technol. 9, 86–113 (2004)

    Google Scholar 

  26. Yoshioka, S., Aso, Y., Izutsu, K.-I., Terao, T.: Stability of ß-galactosidase, a model protein drug, is related to water mobility as measured by O nuclear magnetic resonance (NMR). Pharm. Res. 10(1), 103–108 (1993)

    Article  Google Scholar 

  27. Corzo, G., Revah, S.: Production and characteristics of the lipase from Yarrowia lipolityca 681. Bioresour. Technol. 70, 173–180 (1999)

    Article  Google Scholar 

  28. Brigida, A.I.S., Amaral, P.F.F., Coelho, M.A.Z., Goncalves, L.R.B.: Lipase from Yarrowia lipolityca: production, characterization and application as an industrial catalyst. J. Mol. Catal. B Enzym. 101, 148–158 (2014)

    Article  Google Scholar 

  29. Aloulou, A., Puccinelli, D., De Caro, A., Leblond, Y., Carriere, F.: A comparative study on two fungal lipases from Thermomyces lanuginosus and Yarrowia lipolytica shows the combined effects of detergents and pH on lipase adsorption and activity. Biochim. Biophys. Acta 1771, 1446–1456 (2007)

    Article  Google Scholar 

  30. Aloulou, A., Rodriguez, J., Puccinelli, D., Mouz, N., Leclarine, J., Leblond, Y., Carriere, F.: Purification and biochemical characterization of the LIP2 lipase from Yarrowia lipolytica. Biochim. Biophys. Acta 1771, 228–237 (2007)

    Article  Google Scholar 

  31. Yu, M.R., Lange, S., Richter, S., Tan, T.W., Schmid, R.D.: High-level expression of extracellular lipase Lip2 from Yarrowia lipolytica in Pichia pastoris and its purification and characterization. Protein Expr. Purif. 53, 255–263 (2007)

    Article  Google Scholar 

  32. Antczak, M.S., Kubiak, A., Antczak, T., Bielecki, S.: Enzymatic biodiesel synthesis-key factors affecting efficiency of the process. Renew. Energy 34, 1185–1194 (2009)

    Article  Google Scholar 

  33. Ban, K., Kaieda, M., Matsumoto, T., Kondo, A., Fukuda, H.: Whole-cell biocatalyst for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support particles. Biochem. Eng. J. 8, 39–43 (2001)

    Article  Google Scholar 

  34. Zeng, J., Du, W., Liu, X., Dai, L.: Study on the effect of cultivation parameters and pretreatment on Rhizopus oryzae cell-catalyzed transesterification of vegetable oils for biodiesel production. J. Mol. Catal. B Enzym. 43, 15–18 (2006)

    Article  Google Scholar 

  35. Hlavsova, K., Zarevucka, M., Wimmer, Z., Mackova, M., Sovova, H.: Geotrichum candidum 4013: extracellular lipase versus cell-bound lipase from the single strain. J. Mol. Catal. B Enzym. 61, 188–193 (2009)

    Article  Google Scholar 

  36. Halim, R., Danquah, M.K., Webley, P.A.: Extraction of oil from microalgae for biodiesel production: a review. Biotechnol. Adv. 30(3), 709–732 (2012)

    Article  Google Scholar 

  37. Yang, F., Cheng, C., Long, L., Hu, Q., Jia, Q., Wu, H., Xiang, W.: Extracting lipids from several species of wet microalgae using ethanol at room temperature. Energy Fuels 29, 2380–2386 (2015)

    Article  Google Scholar 

Download references

Acknowledgments

This work was financially supported by Graduate School of Prince of Songkla University and Thai government under Grant No. AGR590148S-1. The first author was supported by grant fund under the program Strategic Scholarships for Frontier Research Network for the Ph.D. Program Thai Doctoral degree from the Office of the Higher Education Commission. The second and third authors were supported by Thai Research Fund under Grant No. RTA5780002.

Author information

Authors and Affiliations

  1. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat-Yai, 90112, Thailand

    Yasmi Louhasakul, Benjamas Cheirsilp & Poonsuk Prasertsan

Authors
  1. Yasmi Louhasakul
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Benjamas Cheirsilp
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Poonsuk Prasertsan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Benjamas Cheirsilp.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Louhasakul, Y., Cheirsilp, B. & Prasertsan, P. Valorization of Palm Oil Mill Effluent into Lipid and Cell-Bound Lipase by Marine Yeast Yarrowia lipolytica and Their Application in Biodiesel Production. Waste Biomass Valor 7, 417–426 (2016). https://doi.org/10.1007/s12649-015-9451-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12649-015-9451-7

Keywords

Search

Navigation