Abstract
This study evaluated the potential of alternative treatments against larval stages of Lernaea cyprinacea. For in vitro test, the nanoemulsified oils of Pinus sp. acicule and oleoresin were evaluated, along with Biogermex® (commercial product based on citrus biomass). For this, the motility of five larvae of the same stage (nauplii or copepodite) were evaluated in a 96-well microplate. Using the best results, on the in vivo test, fries of Rhamdia quelen were submitted to a long-term immersion bath (96 h) containing different concentrations of the product diluted directly in the water. It was possible to notice the antiparasitic potential of the oleoresin and the acicule of Pinus sp., as well as the citrus biomass extract against the parasites. The nanoemulsified oils successfully inhibited the development of nauplii (10 mg L−1 in 24 h) and the fries showed to be tolerant to the presence of the compound (LC50 96 h—16.74 mg L−1). The concentration of 30.5 mg L−1 of Biogermex® eliminated the copepodites within 24 h, being more efficient than Pinus sp. when tested at the same stage, at the times analyzed. The results obtained indicate a potential use of these compounds as prophylactic agents against L. cyprinacea.
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Data Availability
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
The authors thank the National Council for Scientific and Technological Development (CNPq) [M.L. Martins (CNPq 305869/ 2014-0, 306635/2018-6) e CNPq 446072/ 2014-1)]; the Coordination of Improvement of Higher Education Personnel, Brazil (CAPES)—Finance Code 001 for financed part of this study; CAPES for Master Science Scholarship to W.E. Furtado. Also, the authors thank the Company of Agricultural Research and Rural Extension of Santa Catarina (EPAGRI/Cedap) for the donation of the animals used in this study.
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WF worked on the assessment, system`s maintenance, processing of the material analyzed, and wrote the first draft of the manuscript. LC, PM, NL and EB were the technical team who assisted during the assessment of the alternative treatments. MM, FB, and NM contribute with the data analysis and article editing. All authors contributed to the article and approved the submitted version.
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All the animal procedures used in this project was approved by the Ethic Committee on Animal Use of Federal University of Santa Catarina (CEUA/UFSC/PP00928).
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Furtado, W.E., Cardoso, L., de Medeiros, P.B. et al. Antiparasitic potential of alternative treatments against larval stages of Lernaea cyprinacea. J Parasit Dis 45, 1096–1105 (2021). https://doi.org/10.1007/s12639-021-01400-2
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DOI: https://doi.org/10.1007/s12639-021-01400-2