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Improved Viability of Microencapsulated Probiotics in a Freeze-Dried Banana Powder During Storage and Under Simulated Gastrointestinal Tract

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Abstract

Freeze-dried banana powder represents an ideal source of nutrients and has not yet been used for probiotic incorporation. In this study, microencapsulation by freeze drying of probiotics Lactobacillus acidophilus and Lactobacillus casei was made using whey protein isolate (WPI), fructooligosaccharides (FOS), and their combination (WPI + FOS) at ratio (1:1). Higher encapsulation yield was found for (WPI + FOS) microspheres (98%). Further, microcapsules of (WPI + FOS) were used to produce a freeze-dried banana powder which was analyzed for bacterial viability under simulated gastrointestinal fluid (SGIF), stability during storage at 4 °C and 25 °C, and chemical and sensory properties. Results revealed that (WPI + FOS) microcapsules significantly increased bacteria stability in the product over 30 days of storage at 4 °C averaging (≥ 8.57 log CFU/g) for L. acidophilus and (≥ 7.61 log CFU/g) for L. Casei as compared to free cells. Bacteria encapsulated in microspheres (WPI + FOS) were not significantly affected by the SGIF, remaining stable up to 7.05 ± 0.1 log CFU/g for L.acidophilus and 5.48 ± 0.1 log CFU/g for L.casei after 90 min of incubation at pH 2 compared to free cells which showed minimal survival. Overall, encapsulated probiotics enriched freeze-dried banana powders received good sensory scores; they can therefore serve as safe probiotics food carriers.

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Funding

This work was supported by Applied Technology Research and Development Project of Harbin in 2017 (2017RAQXJ087).

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Authors and Affiliations

  1. College of Food Science, Northeast Agricultural University, No. 600 Changjiang St. Xiangfang District, Harbin, 150030, China

    Awa Fanny Massounga Bora, Xiaodong Li, Yongming Zhu & Lingling Du

  2. Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, No. 600 Changjiang St. Xiangfang Dist, Harbin, 150030, China

    Awa Fanny Massounga Bora, Xiaodong Li, Yongming Zhu & Lingling Du

Authors
  1. Awa Fanny Massounga Bora
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  2. Xiaodong Li
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  3. Yongming Zhu
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Correspondence to Xiaodong Li.

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This article does not contain studies with human or animal subjects.

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The authors declare that they have no conflict of interest.

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Massounga Bora, A.F., Li, X., Zhu, Y. et al. Improved Viability of Microencapsulated Probiotics in a Freeze-Dried Banana Powder During Storage and Under Simulated Gastrointestinal Tract. Probiotics & Antimicro. Prot. 11, 1330–1339 (2019). https://doi.org/10.1007/s12602-018-9464-1

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  • DOI: https://doi.org/10.1007/s12602-018-9464-1

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