Skip to main content
Log in

Laboratory experiment to measure 5-MHz volume backscattering strengths from Red-tide causing microalgae Chattonella antiqua

  • Article
  • Published:
Ocean Science Journal Aims and scope Submit manuscript

Abstract

An acoustic laboratory experiment using 5-MHz signals was conducted to measure the volume backscattering strengths of red-tide causing microalgae, Chattonella antiqua, which is one of the species of harmful algal blooms in the coastal waters of Korea and Japan. The measured backscattering strengths increased with cell abundance, with a slope of approximately 10 dB per decade increase in cell numbers. The density and sound speed ratios of the Chattonella cell to the water medium were estimated via the density gradient centrifugation method and the time-travel difference method, respectively. Finally, the measured backscattering strengths were compared to those predicted by a fluid-sphere scattering model, in which the estimated sound speed and density contrasts were used as input parameters.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Anglès S, Jordi A, Garcès E, Masó M, Basterretxea G (2008) High-resolution spatio-temporal distribution of a coastal phytoplankton bloom using laser in situ scattering and transmissometry (LISST). Harmful Algae 7(6):808–816

    Article  Google Scholar 

  • Ahn YH, Shanmugam P (2006) Detecting the red tide algal blooms from satellite ocean color observations in optically complex Northeast-Asia Coastal waters. Remote Sens Environ 103(4): 419–437

    Article  Google Scholar 

  • Bok TH, Paeng DG, Kim E, Na J, Kang D (2010) Ultrasound backscattered power from Cochlodinium polykrikoides, the main red tide species in the Southern Sea of Korea. J Plankton Res 32(4):503–514

    Article  Google Scholar 

  • Blanc S, Benitez CE, De Milou MIE, Mosto P, Lascalea G, Juarez RE (2000) Acoustical behaviour of phytoplanktonic algae. Acoust Lett 23(9):175–182

    Google Scholar 

  • Chu D, Wiebe P, Copley N (2000) Inference of material properties of zooplankton from acoustic and resistivity measurements. ICES J Mar Sci 57:1128–1142

    Article  Google Scholar 

  • De Jonge VN (1979) Quantitative separation of benthic diatoms from sediments using density gradient centrifugation in the colloidal silica Ludox-TM. Mar Biol 51:267–278

    Article  Google Scholar 

  • Eppley RW, Holmes RW, Strickland JDH (1967) Sinking rates of marine phytoplankton measured with a fluorometer. J Exp Mar Biol Ecol 1(2):191–208

    Article  Google Scholar 

  • Foote K (1990) Speed of sound in Euphausia superba. J Acoust Soc Am 87(4):1405–1408

    Article  Google Scholar 

  • Guillard RRL (1975) Culture of Marine Invertebrate Animals. Plenum Press, New York, 338 p

    Google Scholar 

  • Hinton R, Dobrota M (1976) Density gradient centrifugation North-Holland, Amsterdam, 290 p

  • Johnson RK (1977) Sound scattering from a fluid sphere revisited. J Acoust Soc Am 61(2):375–377

    Article  Google Scholar 

  • Kim E, Lee H, Na J, Choi JW, Kang D (2010) 5-MHz acousticbackscatter measurements of Cochlodinium polykrikoides blooms in Korean coastal waters. ICES J Mar Sci 67:1759–1765

    Article  Google Scholar 

  • Kim SY, Seo KS, Lee CG, Lee Y (2007) Diurnal Modification of a Red-Tide Causing Organism Chattonella antiqua (Raphidophyceae) from Korea. Algae 22(2):95–106

    Article  Google Scholar 

  • Krautkrämer J, Krautkrämer H (1990) Ultrasonic Testing of Materials. 4th edn, Springer-Verlag, Berlin, 677 p

    Google Scholar 

  • Marshall JA, Hallegraeff GM (1999) Comparative ecophysiology of the harmful alga Chattonella marina (Raphidophyceae) from South Australian and Japanese waters. J Plankton Res 21(10):1809–1822

    Article  Google Scholar 

  • Medwin H, Clay CS (1998) Fundamentals of Acoustical Oceanography. Academic Press, New York, 712 p

    Google Scholar 

  • Mukai T, Iida K, Ando Y, Mikami H, Maki Y, Matsukura R (2004) Measurements of Swimming Angles, Density, and Sound Speed of the Krill Euphausia Pacifica for Target Strength Estimation. In: Proceedings of MTTS/IEEE Techno-Ocean’04, Kobe, Japan, 9–12 Nov 2004, pp 383–388

  • NFRDI (2012) Standard of harmful algal blooms forecast in Korea. http://portal.nfrdi.re.kr/redtide/webpage/ope-ration/operation_04.jsp. Accessed 08 Mar 2012

  • Oliver RL, Kinnear AJ, Ganf GG (1981) Measurements of cell density of three freshwater phytoplankters by density gradient centrifugation. Limnol Oceanogr 26(2):285–294

    Article  Google Scholar 

  • Olenina I, Hajdu S, Edler L, Andersson A, Wasmund N, Busch S, Göbel J, Gromisz S, Huseby S, Huttunen M, Jaanus A, Kokkonen P, Ledaine I, Niemkiewicz E (2006) Biovolumes and size-classes of phytoplankton in the Baltic Sea. HELCOM Baltic Sea Environment Proceedings No 106, 144 p

  • Park JS, Kim TA, Lee SK, Kim HG (1987) Study on red tide occurrence and its succession in Korea. 69th Report of National Fisheries Research and Development Institute

  • Peeters H (1979) Separation of cells and subcellular elements. Pergamon Press, Oxford, 116 p

    Google Scholar 

  • Price CA, Mendiola-morgenthaler LR, Goldstein M, Breden EN, Guillard RRL (1974) Harvest of planktonic marine algae by centrifugation into gradients of silica in the cf-6 continuousflow zonal rotor. Biol Bull 147:136–145

    Article  Google Scholar 

  • Robbins IC, Kirkpatrick GJ, Blackwell SM, Hillier J, Knight CA, Moline MA (2006) Improved monitoring of HABs using autonomous underwater vehicles (AUV). Harmful Algae 5(6):749–761

    Article  Google Scholar 

  • Stæhr PA, Markager S, Sand-Jensen K (2004) Pigment specific in vivo light absorption of phytoplankton from estuarine, coastal and oceanic waters. Mar Ecol Prog Ser 275:115–128

    Article  Google Scholar 

  • Urick RJ (1998) Principles of Underwater Sound. 3rd edn. McGraw-Hill, New York, 444 p

    Google Scholar 

  • Van Ierland ET, Peperzak L (1984) Separation of marine seston and density determination of marine diatoms by density gradient centrifugation. J Plankton Res 6(1):29–44

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jee Woong Choi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, J., Choi, J.W. & Kang, D. Laboratory experiment to measure 5-MHz volume backscattering strengths from Red-tide causing microalgae Chattonella antiqua . Ocean Sci. J. 47, 173–179 (2012). https://doi.org/10.1007/s12601-012-0017-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12601-012-0017-3

Key words

Navigation